This set of Pharmaceutical Biotechnology Multiple Choice Questions & Answers (MCQs) focuses on “Protein Engineering”.
1. Which is considered as the foremost step in Protein Engineering?
a) Insertion of nucleotides
b) Gene encoding
c) A study of its three-dimensional structure
d) Modification in its prosthetic group
View Answer
Explanation: The first and the foremost step in Protein Engineering is to study the protein’s three-dimensional structure for a better understanding of its usage and other applications. This can be accomplished by using X-ray crystallography and NMR Spectroscopy.
2. The main objective of Protein Engineering is to produce more valuable and useful proteins by altering their pre-existing structures.
a) True
b) False
View Answer
Explanation: Protein Engineering is primarily involved in producing proteins having relatively more efficiency, in terms of their function and usability. This is achieved by modifying their structures with the help of different biological techniques.
3. Who received the noble prize for determining the 3D structure of penicillin in 1964?
a) Alexander Fleming
b) Dorothy Crowfoot Hodgkin
c) Ernst Boris Chain
d) Howard Walter Florey
View Answer
Explanation: Dorothy Crowfoot Hodgkin was awarded the noble prize in the year 1964 for determining the 3D structure of the antibiotic named penicillin, discovered by Alexander Fleming, using X-ray crystallography.
4. Which of the following is an evolutionary method used in Protein Engineering?
a) Target evolution mutagenesis
b) Site directed mutagenesis
c) Artificial genome synthesis
d) DNA shuffling
View Answer
Explanation: DNA shuffling is considered as the earliest approach for altering amino acids. It is an invitro recombination technique primarily involved in Direct evolution process. Here, the homologous genes are fragmented and purified to produce highly recombined genes.
5. Which of the following technique is not involved in Protein Engineering?
a) Selection
b) Conjugation
c) Mutagenesis
d) Recombination Technology
View Answer
Explanation: The three primary methods involved in Protein Engineering are Selection, Mutagenesis and Recombination Technology, whereas, the process of conjugation allows protein molecules to modify its biochemical properties.
6. Protein Engineering is not applicable for which of the following?
a) Detergent industry
b) Biopolymer production
c) Dyeing industry
d) Cancer Treatment
View Answer
Explanation: Protein Engineering has diverse applications in various fields ranging from medicine to nanobiotechnology. They are also involved in industrial processes such as detergent and biopolymer productions, but there is no firm proof of its usage in dyeing industry.
7. Which of the following involves blocking the activity of peptides?
a) Peptidomimetics
b) Mutagenesis
c) Overlap extension
d) Translation systems
View Answer
Explanation: Peptidomimetics are compounds that mimics the natural peptides or has the affinity to block its activity after binding to them. An example of peptidomimetics is the alkaloid derived from plants known as morphine.
8. Overlap extension is a common method of site directed mutagenesis.
a) True
b) False
View Answer
Explanation: Site directed mutagenesis allow us to modify a particular gene by inserting or deleting the amino acid sequences. It involves two common methods; one is Overlap extension and the other one is Whole plasmid mutagenesis.
9. What is the full form of GFP?
a) Grey Fluorescent Protein
b) Grey Folded Protein
c) Green Fluorescent Protein
d) Green Folded Protein
View Answer
Explanation: GFP is the abbreviation for Green Fluorescent Protein. It plays a crucial role in research and other biological studies. It comprises of 238 amino acid sequences and are primarily used as markers to track other proteins.
10. Which of the following is not a disadvantage of directed evolution method?
a) Randomized gene recombination
b) Restriction to access the protein libraries
c) Limited protein knowledge
d) Scaling up difficulties
View Answer
Explanation: Even though, directed evolution is considered to be an important strategy in Protein Engineering, it has remarkable disadvantages, but the method of Randomized gene recombination is not one of them, as it allows us to achieve a high level of genetic diversity.
Sanfoundry Global Education & Learning Series – Drug and Pharmaceutical Biotechnology.
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