Protein Engineering Questions and Answers

Protein Engineering Interview Questions and Answers

Our 1000+ Protein Engineering questions and answers focuses on all areas of Protein Engineering subject covering 100+ topics in Protein Engineering. These topics are chosen from a collection of most authoritative and best reference books on Protein Engineering. One should spend 1 hour daily for 2-3 months to learn and assimilate Protein Engineering comprehensively. This way of systematic learning will prepare anyone easily towards Protein Engineering interviews, online tests, examinations and certifications.


– 1000+ Multiple Choice Questions & Answers in Protein Engineering with explanations
– Every MCQ set focuses on a specific topic in Protein Engineering Subject

Who should Practice these Protein Engineering Questions?
– Anyone wishing to sharpen their knowledge of Protein Engineering Subject
– Anyone preparing for aptitude test in Protein Engineering
– Anyone preparing for interviews (campus/off-campus interviews, walk-in interview and company interviews)
– Anyone preparing for entrance examinations and other competitive examinations
– All – Experienced, Freshers and Students


Here’s list of Questions & Answers on Protein Engineering Subject covering 100+ topics:

1. Questions & Answers on Structure of Protein

The section contains questions and answers on amino acids, protein molecule bonds, protein primary and secondary structures, protein tertiary and quaternary structures.

Amino Acids – 1
Amino Acids – 2
Primary Structure of Protein
Secondary Structure of Protein
Tertiary Structure of Protein
Quaternary Structure of Protein
Bonds that Stabilize a Protein Molecule

2. Questions on Genetic Code and Translation Modifications

The section contains questions on genetic code, t-rna and its aminoacylation, ribosomes, translation and post-translational modifications, ramachandran plot and protein folding.

The Genetic Code
t-RNA and its Aminoacylation
Post-Translational Modifications – 1
Post-Translational Modifications – 2
Ramachandran Plot
Protein Folding – 1
Protein Folding – 2

3. Questions & Answers on Protein Degradation in Cell

The section contains questions and answers on ubiquitin-proteasome pathway of protein degradation and lysosome mediated protein degradation.

Ubiquitin-Proteasome Pathway of Protein Degradation Lysosome Mediated Protein Degradation

4. Questions on Structural Study of Proteins

The section contains questions on protein crystallization methods, x-ray crystallography, nmr and mass spectroscopy, maldi-tof and esi-ms.

Methods of Protein Crystallization – 1
Methods of Protein Crystallization – 2
X-ray Crystallography – 1
X-ray Crystallography – 2
NMR Spectroscopy
Mass Spectroscopy – 1
Mass Spectroscopy – 2

5. Questions & Answers on Structure Function Relationships in Proteins

The section contains questions and answers on hth motif, homeodomain, zinc fingers, leucine zippers, integral and lipid anchored membrane proteins, peripheral membrane proteins, bacteriorhodopsin, photosynthetic reaction center, transmembrane proteins structure and function.

HTH Motif and Homeodomain
Zinc Fingers
Leucine Zippers
Integral Membrane Proteins
Lipid Anchored Membrane Proteins
Peripheral Membrane Proteins
Structure and Function of Transmembrane Proteins-1
Structure and Function of Transmembrane Proteins-2
Photosynthetic Reaction Center

6. Questions on Designing a New Protein Molecule

The section contains questions on peptides chemical synthesis, protein engineering cycle, de-novo protein design, dna shuffling, error prone pcr and phage display.

Chemical Synthesis of Peptides – 1
Chemical Synthesis of Peptides – 2
Protein Engineering Cycle
Rational Approach for De-novo Protein Design
DNA Shuffling
Error Prone PCR
Phage Display

7. Questions & Answers on Targets of Protein Engineering

The section contains questions and answers on engineering enzymes and stability, engineering specificity and purification, industrial enzymology, protein engineering for healthcare, engineering antibodies, hormones and receptors.

Engineering Enzymes
Engineering Stability
Engineering Specificity and Purification
Engineering Antibodies, Hormones, and Receptors
Industrial Enzymology
Protein Engineering for Healthcare

8. Questions on Protein Engineering for Biosensors

The section contains questions on chemically engineered electronic proteins, genetically fused proteins, gene engineering for molecular networking and protein assembly.

Chemically Engineered Electronic Proteins
Genetically Fused Proteins
Gene Engineering for Molecular Networking
Gene Engineering for Protein Assembly

9. Questions & Answers on Applications of Protein Engineering

The section contains questions and answers on antibody protein engineering, insulin, engineering blood clotting factor viii, affinity purification, vaccine development, tyrosyl trna synthetase, proteins enhance solubility and assist folding.

Protein Engineering of Antibody – 1
Protein Engineering of Antibody – 2
Protein Engineering for Affinity Purification – 1
Protein Engineering for Affinity Purification – 2
Engineering Therapeutic Hormone: Insulin
Engineering Blood Clotting Factor VIII – 1
Engineering Blood Clotting Factor VIII – 2
Engineering in Vaccine Development
Engineering Tyrosyl-tRNA Synthetase
Engineering to Enhance Solubility and Assist Folding of Proteins

10. Questions on Molecular Bioscreening in Oncology

The section contains questions on drug discovery, molecular target selection, suitable assay system and drug discovery screening.

Mechanism Based Drug Discovery
Choosing an Appropriate Molecular Target
A Suitable Assay System
Screening of Drug Discovery
If you would like to learn Protein Engineering thoroughly, you should attempt to work on the complete set of questions and answers mentioned above. It will immensely help anyone trying to crack an exam or an interview.


Here’s the list of Best Reference Books in Protein Engineering.

Wish you the best in your endeavor to learn and master Protein Engineering!

Manish Bhojasia, a technology veteran with 20+ years @ Cisco & Wipro, is Founder and CTO at Sanfoundry. He is Linux Kernel Developer & SAN Architect and is passionate about competency developments in these areas. He lives in Bangalore and delivers focused training sessions to IT professionals in Linux Kernel, Linux Debugging, Linux Device Drivers, Linux Networking, Linux Storage, Advanced C Programming, SAN Storage Technologies, SCSI Internals & Storage Protocols such as iSCSI & Fiber Channel. Stay connected with him @ LinkedIn