This set of Pharmaceutical Biotechnology Multiple Choice Questions & Answers (MCQs) focuses on “Approaches to Stabilizing Proteins”.
1. Which factor does not affect the stability in proteins?
a) Temperature
b) Hydrogen Bonding
c) moisture content
d) pH
View Answer
Explanation: Environmental factors such as temperature, pH, hydrogen bonding and other factors such as conformational changes can affect the stability of proteins but moisture content is not an essential factor to be considered for protein stability.
2. Proteins cannot be stabilized by using natural methods.
a) True
b) False
View Answer
Explanation: When it comes to protein stabilization, various methods are approached and one of them being the natural method. In this mode of stabilization, the structure of the protein is stabilized with the help of naturally available enzymes.
3. Which of the following method is not involved in determining protein stabilization?
a) Freeze-drying
b) Crystallization
c) Nuclear Magnetic Resonance
d) Aerosol drying
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Explanation: Nuclear Magnetic Resonance is a method that is mainly involved in determining the structure of the protein when placed under magnetic field. Whereas, all other methods are commonly used for the purpose of stabilization.
4. Which of the following solvent is used to maintain the stability of protein in aqueous medium?
a) Glycerol
b) Propylene glycol
c) Ethylene glycol
d) Diethylene glycol
View Answer
Explanation: The stability of protein molecules in aqueous medium is maintained or improved by incorporating solvents such as glycerol in it. Glycerol is also known for its ability to restore structure of protein in its compatible form
5. During cryopreservation, the proteins are stabilized under which temperature range?
a) 0 to -10 °C
b) -20 to -190 °C
c) -5 to -18 °C
d) 5 to -18 °C
View Answer
Explanation: During the process of cryopreservation, the proteins are immersed within the solutions containing preservatives and are kept under the temperature range -20 to -190 °C in order to stabilize its structure for storage.
6. Which technique is widely used for stabilizing proteins in its native form?
a) Aerosol Drying
b) Circular Dichroism
c) Differential Scanning Calorimetry
d) Crystallization
View Answer
Explanation: Differential Scanning Calorimetry measures the heat required for the protein to be denatured. Therefore, it allows us to characterize the protein stabilization in its native form.
7. Which of the following force is not involved in stabilizing proteins?
a) Hydrophilicity
b) Hydrogen Bonding
c) Vander Waals Interaction
d) Disulfide Bonds
View Answer
Explanation: The forces that are involved in stabilizing proteins include Hydrogen Bonding, Vander Waals Interaction, Disulfide Bonds, Hydrogen Bonding, Ionic interactions and Hydrophobicity. Whereas, Hydrophilicity typically explains the affinity of the molecule to attract water.
8. Some proteins may not be stable in the aqueous solutions, even when they are stored at low temperatures.
a) True
b) False
View Answer
Explanation: Proteins in general have a complex structure. Hence, even in lower temperatures, some proteins may not attain its stable form. This challenges the pharmacists to determine the dosage form of therapeutic proteins.
9. Which of the following enzyme does not involve in the process of protein stabilization?
a) Amino acylase
b) Alcohol dehydrogenase
c) β-lactamase
d) γ-lactamase
View Answer
Explanation: β-lactamase enzymes does not necessarily involve in the mechanism of protein stabilization but they are used to disintegrate the molecule’s antibacterial properties by undergoing hydrolysis process.
10. Protein stability in general refers to which of the following?
a) Its thermal stability
b) Its chemical stability
c) Its dynamic stability
d) Protein-Protein interaction
View Answer
Explanation: Generally, Protein stability refers to its thermal stability rather than others. Because by identifying the temperature at which protein undergoes denaturation, we can easily characterize its stability and thereby prescribe the dosage form.
Sanfoundry Global Education & Learning Series – Drug and Pharmaceutical Biotechnology.
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