This set of Bioseparation Processes Multiple Choice Questions & Answers (MCQs) focuses on “Electrophoresis”.
1. Electrophoresis is the process of separation of charged solutes based on _____________
a) electrophoretic mobility
b) molecular weight
c) charge
d) density
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Explanation: The process of electrophoresis is the separation of solutes that are charged and the separation is based upon the electrophoretic mobility of the solute which means the separation is dependent on the movement of charged molecules in comeback to an electric field.
2. The _________ process of separation gives a really excellent separation of solute.
a) filtration
b) sedimentation
c) electrophoresis
d) chromatography
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Explanation The process of electrophoresis is the separation method that gives a really excellent separation of solute and is broadly used for protein and nucleic acid purification and analysis.
3. Electrophoresis is carried out by adding the mixture of solute to be separated using a medium which conducts __________
a) charges
b) electricity
c) molecules
d) solvent
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Explanation: Electrophoresis is passed by addition the combination of solutes to be separated into a medium that conducts electricity followed by the presentation of an electric field across the used medium.
4. The positively charged solute migrates towards _________ in the process of electrophoresis.
a) negative electrode
b) positive electrode
c) neutral
d) doesn’t migrate at all
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Explanation: The positively charged solutes migrate towards the negative electrode while the negatively charged solutes migrate towards the positive electrode.
5. The neutral solutes shows no movement because of ___________
a) applied pressure
b) applied electric field
c) current
d) molecular weight
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Explanation: The neutral solutes show no movement because of the applied electric field though they can diffuse and show movement due to concentration gradient.
6. The process of electrophoresis separation is classified on the basis of type of medium as __________
a) gel electrophoresis
b) liquid phase electrophoresis
c) gel and liquid phase electrophoresis
d) electrophoresis
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Explanation: The process of electrophoresis separation is classified on the basis of type of medium as gel electrophoresis and free flow i.e. liquid phase electrophoresis. In gel electrophoresis, the medium used for the separation process is gel and for the liquid phase, the medium is solution which helps in the separation process.
7. The material agarose, starch are used for the separation process in the liquid phase electrophoresis.
a) True
b) False
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Explanation: The material agarose, starch are used for the separation process in the gel electrophoresis not liquid electrophoresis. This form gel with high content of water as well as allows the passage of solutes having large size through the structures that are porous.
8. In the process of gel electrophoresis, the molecules are separated into __________
a) bands
b) gels
c) phases
d) solution
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Explanation: The bands are obtained when the molecules are separated in the process of gel electrophoresis and the obtained bands have to be extracted subsequently from the specific area within the gel.
9. The most common gel electrophoresis method is __________
a) GE
b) PEG
c) PAGE
d) AGE
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Explanation: The method of gel electrophoresis is known as PAGE i.e. PolyAcrylamide Gel Electrophoresis and it separates the proteins though vertical gels. In this the charges on protein is dependent on the pH of the medium used which means that the proteins will be neutral at their isoelectric point, will be positive below the isoelectric point and negative above the isoelectric point.
10. The native gel electrophoresis separation is should be negatively charged.
a) True
b) False
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Explanation: The native gel electrophoresis separation is should be negatively charged and is done by maintaining the buffer pH and the isoelectric pH of all the proteins must be above the gel medium. At the points where this is not possible, then the maintenance of pH is below the isoelectric points of the proteins within the sample and is referred to reversed polarity native PAGE.
Sanfoundry Global Education & Learning Series – Bioseparation Processes.
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