This set of Bioseparation Processes Multiple Choice Questions & Answers (MCQs) focuses on “Field-Flow Fractionation”.
1. A type of biological separation process used to separate particles using field is ___________
a) field flow fractionation
b) electrophoresis
c) chromatography
d) ultrafiltration
View Answer
Explanation: Field flow fractionation is a process of biological separation which separates particles on the basis of size using the fields like electric field, magnetic field, hydraulics field, gravitational field etc.
2. The fields are applied to a diluted __________ in the fluid in the process of field flow fractionation.
a) slurry
b) suspension
c) liquid
d) solid
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Explanation: The fields are applied to a diluted suspension in the fluid in the process of field flow fractionation or it can be pumped as a solution through a channel that should be long as well as narrow and should be perpendicular to the applied field.
3. Field flow fractionation is used to fractionate _________ in the process of bioseparation.
a) small molecules
b) large molecules
c) large macromolecules
d) micro-molecules
View Answer
Explanation: The process of Field flow fractionation in the process of bioseparation is used to separate the large macromolecules in the form of fractions. It also separates the macromolecules that are in the aggregated forms.
4. The flow field fractionation is a flow __________ method having retention applied by the force field.
a) elution
b) retention
c) flow
d) mobile phase
View Answer
Explanation: The flow field fractionation is a flow elution method having retention applied by the force field and the process drives the solutes to a specific region of flow. The separation process is gentler and accurate when compared to the chromatography.
5. A liquid flows in the field flow fractionation instrument in _________ fashion.
a) back flow
b) laminar
c) shallow flow
d) narrow
View Answer
Explanation: The liquid is sent to the field flow instrument in the streamline i.e. the laminar fashion. If the direction of the field is towards the gravity, the flow of liquid will be 45 degree to it.
6. The ___________ in the field flow fractionation is the combined effect of applied field to the process.
a) flow
b) field
c) fractionation
d) channel
View Answer
Explanation: The fractionation in the field flow fractionation is the combined effect of applied field to the process and have a flow in parabolic pattern as the carrier mobile phase.
7. The process of separation in field flow fractionation is carried out in _________ channel.
a) obstructed
b) unobstructed
c) disrupted
d) flowmetry
View Answer
Explanation: The process of separation in field flow fractionation is carried out in an obstructed, slim fit and a ribbon kind of channel which is connected to the rectangular cross section having connection to the capillary tubes in order to have a continuous flow of the liquid.
8. The substances which are been separated by the field flow fractionation are subjected to ___________
a) separation field
b) depletion field
c) flow field
d) no field
View Answer
Explanation: The substances are separated by the field flow fractionation and are subjected to the separation field such as electric field, steric field, permeation field, magnetic field and gravitational field.
9. The separated substances of different species __________ itself in the process of field flow fractionation.
a) diverge
b) flow
c) position
d) dismantle
View Answer
Explanation: The separated substances of different species position itself and are placed at various locations in the field of flow through the channel, in the process of field flow fractionation.
10. Based on __________ the species move to the various locations in the field flow fractionation process.
a) pressure
b) velocity
c) speed
d) flow
View Answer
Explanation: The fluid velocity is the factor for the movement of the species in the various locations in the field flow fractionation process and the fluid velocity is a fundamental understanding for calculation of the flow in the pipe.
Sanfoundry Global Education & Learning Series – Bioseparation Processes.
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