This set of Bioseparation Science Multiple Choice Questions & Answers (MCQs) focuses on “Diffusional Limitations in Adsorption Processes”.
1. Adsorption can be limited by __________
a) rate of solute
b) rate of solvent
c) rate of solution
d) rate of reaction
View Answer
Explanation: The process of adsorption can be limited by the rate of solute transport from the solution known as feed solution to the surface of the adsorbent in the adsorption process of bioseparation engineering.
2. The size and the geometry of the adsorbent is important for ____________
a) thermodynamics
b) reaction
c) transport
d) adsorption kinetics
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Explanation: The size as well as the geometry of the adsorbent material frequently plays a significant role in the adsorption kinetics.
3. The solute transport-limiting mechanisms are observed in __________
a) mass Transport
b) external and internal mass transport
c) external mass transport
d) internal mass transfer
View Answer
Explanation: The solute transport-limiting mechanisms are observed in external mass transport which is controlled by diffusion from the bulk solution to the surface of the adsorbent and the internal mass transport which is controlled by diffusion within a porous adsorbent.
4. The Langmuir isotherm is applicable when __________
a) weak interaction
b) neutral interaction
c) strong specific interaction
d) specific interaction
View Answer
Explanation: The Langmuir isotherm is applicable during a strong specific interaction between the solute and the adsorbent.
5. Which type of adsorption follows Langmuir isotherm?
a) Chromatography
b) Ion exchange chromatography
c) Affinity chromatography
d) Ion exchange and affinity chromatography
View Answer
Explanation: Ion exchange and affinity type adsorptions generally follow Langmuir isotherm. Langmuir isotherm predicts the saturation of the adsorption sites by solute molecules, indicating monolayer formation.
6. What is the significance of the given diagram?
a) Determination of Langmuir isotherm constant
b) Determination of Freundlich isotherm constant
c) Determination of linear isotherm constant
d) Determination of constants
View Answer
Explanation: The Freundlich isotherm does not predict any saturation of the binding surface by the solute. The adsorption of antibiotics, steroids and hormones onto commonly used adsorbents follow this type of isotherm.
7. Which adsorption is applicable for Freundlich isotherm?
a) Hydrophobic interaction
b) Reverse phase
c) Hydrophobic interaction and reverse phase interaction
d) Chromatography
View Answer
Explanation: The reverse phase and hydrophobic interaction type adsorption generally follow Freundlich type isotherm. The constants K and n must be determined experimentally from CB-CV data. It is an empirical correlation of the form shown below: CB = KFCvn.
8. What is the significance of the given diagram?
a) Determination of Langmuir isotherm constant
b) Determination of Freundlich isotherm constant
c) Determination of linear isotherm constant
d) Determination of constants
View Answer
Explanation: The Langmuir isotherm predicts the saturation of the adsorption sites by solute molecules, indicating monolayer formation. CBmax and KL are constants. The slope of the isotherm is KL/CBmax. CBmax is the saturation constant and KLis the affinity constant for Langmuir isotherm.
9. What is the significance of the given diagram?
a) Hydrophobic interaction separation
b) Hydrophilic interaction separation
c) Hydrophobic separation of rEGF
d) Hydrophilic interaction of rEGF
View Answer
Explanation: The analysis of an adsorption process is based on identifying an equilibrium relationship between bound and free solute and performing a solute material balance. The above diagram is the example for the adsorption process of hydrophobic interactions based on the isotherms.
10. The purification of recombinant insulin is based on ___________
a) use of reverse phase adsorption
b) use of equilibrium adsorption
c) use of polar adsorption
d) use of binding molecules
View Answer
Explanation: The purification of recombinant insulin using reverse phase adsorption. Reverse phase adsorption is not particularly suitable for macromolecules such as proteins and nucleic acids since they do not partition very well into the hydrocarbon layer.
Sanfoundry Global Education & Learning Series – Bioseparation Science.
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