This set of Bioseparation Technology Multiple Choice Questions & Answers (MCQs) focuses on “Bioseparation Techniques”.
1. What are the two broad groups of bioseparation?
a) Resolution and productivity
b) Productivity and colour
c) Colour and resolution
d) Selectivity and colour
View Answer
Explanation: The bioseparation are categorized into two broad groups Resolution and productivity. Resolution combines the high selectivity of the biological products and the productivity combines the high throughput of the biological products.
2. Which bioseparation techniques have low-resolution with high throughput?
a) Cell disruption, precipitation, filtration
b) Ultra filtration, ultracentrifugation
c) Chromatography, Adsorption
d) Affinity separation, electrophoresis
View Answer
Explanation: The bioseparation techniques having low resolution and high throughput are cell disruption, precipitation, and filtration. Cell disruption is the process of extraction of product from the cell. Precipitation is the process of settling of solid part of the solution which contains the product. Filtration is the process of separation of impurities from the product of interest. These techniques utilize the equipments of classical biological process and do not focus on the resolution of the output.
3. Which bioseparation techniques have high-resolution and low throughput?
a) Cell disruption, precipitation, filtration
b) Ultra filtration, ultracentrifugation
c) Chromatography, adsorption
d) Affinity separation, electrophoresis, chromatography
View Answer
Explanation: The bioseparation techniques having high resolution with low throughput are affinity separation, electrophoresis and chromatography. The rate of production of the purified product is low for these techniques but the quality of purification is good. They utilize latest equipments with advanced monitoring options. Affinity separation requires column to separate the products. Electrophoresis uses electric field for the separation of products. Chromatography includes some instruments like HPLC which uses mobile phase and stationary phase for the separation of products.
4. What is RIPP scheme in bioseparation?
a) Recovery, Isolation, Purification, Polishing
b) Reuse, Isolation, Polishing, Packaging
c) Recovery, Isolation, Production, Packaging
d) Reuse, Isolation, Production, Polishing
View Answer
Explanation: The RIPP scheme stands for Recovery of the product from the fermentation broth followed by isolation of the product from the cells, followed by purification of the desired product and finally polishing of the product for final packaging. The scheme follows low-resolution techniques for recovery and isolation then followed by high resolution techniques. The high throughput and low resolution are used reduce the volume and concentration of the product. The high resolution and low throughput are used to produce pure and polished products.
5. The exception to RIPP scheme is ___________
a) ultra filtration, ultra centrifugation
b) electrophoresis
c) affinity chromatography, expanded bed adsorption
d) leaching, precipitation
View Answer
Explanation: The exception to recovery, isolation, purification and polishing steps are affinity chromatography and expanded bed adsorption. In expanded bed adsorption, the recovery and isolation of product is a single step process. In affinity chromatography, isolation and purification of product is a single step process.
6. What are the important factors for designing bioseparation process?
a) Nature of starting material, location of product, abundance of product, susceptibility of product to degradation, desired physical form of product, quality requirement, process economics
b) Nature of starting material, location of product, abundance of product, process economics
c) Susceptibility of product to degradation, desired physical form of product, process economics
d) Nature of starting material, quality requirement, process economics
View Answer
Explanation: The development of the bioseparation process involves several considerations which includes nature of starting material e.g. Cell suspension or crude solution of protein etc. ; location of product e.g. the product is intracellular or extracellular or embedded in the material etc.; abundance of product e.g. the concentration of product and the impurities; susceptibility of product to degradation e.g. the pH of the product, sensitivity of the product; desired physical form of product e.g. lyophilized product or solution or suspension etc.; quality requirement e.g. percentage of product purity or absence of impurities etc.; process economics i.e. the costing of the total bioseparation.
7. Separation of monoclonal antibody from cell culture supernatant is an example of Bioseparation.
a) True
b) False
View Answer
Explanation: The separation of monoclonal antibody involves purification of cell culture supernatant through membrane filtration followed by affinity chromatography, membrane filtration helps to avoid clogging in the column of affinity chromatography, further ion-exchange chromatography is used to purify the cells and finally gel-filtration is used for polishing.
8. What are the disadvantages of using RIPP scheme?
a) High capital cost, high operations cost, lower recovery of product
b) Low capital cost, high operation cost, high recovery
c) High capital cost, low operation cost, high recovery of the product
d) Low capital cost, low operation cost, low recovery of product
View Answer
Explanation: The recovery, isolation, purification and polishing requires more capital cost and operation cost which leads to increase in the product economics and it leads to lack of proper product recovery.
9. Which is the method introduced in RIPP scheme to give high throughput to the process?
a) Filtration
b) Sedimentation
c) Microfiltration
d) Membrane separation
View Answer
Explanation: Membrane separation is the method used to give high throughput to the process of purification and it fine tunes the process as well as gives high selectivity to the product purification. It is one of the most popular process now-a-days due to its simple approach and efficiencies of separation.
10. What are the emerging technologies in the field of bioseparation?
a) Membrane chromatography, expanded bed chromatography, high resolution ultra filtration, hybrid bioseparation
b) Chromatography, filtration, sedimentation
c) Membrane chromatography, packed bed filtration, ion-exchange chromatography
d) Microfiltration, zonal centrifugation, ultra filtration
View Answer
Explanation: The latest technologies have high throughput and have high selectivity for the purification than the conventional methods of separation. The techniques like membrane chromatography, expanded bed chromatography, high resolution ultra filtration and hybrid bioseparation have shown better purification when compared to the basic bioseparation techniques.
Sanfoundry Global Education & Learning Series – Bioseparation Technology.
To practice all areas of Bioseparation Technology, here is complete set of 1000+ Multiple Choice Questions and Answers.
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