1. What is bioseparation engineering?
a) A biological separation which involves engineering approach
b) A reaction and separation approach to study the biological compounds
c) A separation and purification segment which followed some form of biological reaction
d) A systematic study of the scientific and engineering principle utilized for the large scale purification of biological products
Explanation: Bioseparation engineering is the scientific study of engineering principles used in separation processes which are utilized for the large scale purification of biological products.
2. What is the primary objective of bioseparation technology?
a) Separation of biomolecules from complex mixtures
b) Exploration of marine life
c) Study of plant genetics
d) Development of synthetic materials
Explanation: Bioseparation technology focuses on the isolation and purification of biomolecules from diverse biological sources.
3. What is separated in bioseparation?
a) Enzymatically derived products
b) Mechanically derived products
c) Biologically derived products
d) Chemically derived products
Explanation: Bioseparation is the scientific study of engineering principles used in separation processes which are utilized for the large scale purification of biologically derived products.
4. What are the emerging technologies in the field of bioseparation?
a) Microfiltration, zonal centrifugation, ultra filtration
b) Membrane chromatography, packed bed filtration, ion-exchange chromatography
c) Chromatography, filtration, sedimentation
d) Membrane chromatography, expanded bed chromatography, high resolution ultra filtration, hybrid bioseparation
Explanation: The latest technologies have high throughput and have high selectivity for the purification than the conventional methods of separation. The techniques like membrane chromatography, expanded bed chromatography, high resolution ultra filtration and hybrid bioseparation have shown better purification when compared to the basic bioseparation techniques.
5. What is the importance of bioseparation?
a) Removal of impurities, prevention of product degradation, reduction in bulk
b) Enrichment of target products, achievement of product specification, reduction in bulk, removal of impurities, prevention of product degradation
c) Enrichment of target products, achievement of product specification, reduction in bulk
d) Reduction in bulk, achievement of product specification, prevention of product degradation
Explanation: The bioseparation is important as it helps in Enrichment of target products, achievement of product specification, reduction in bulk, removal of impurities and prevention of product degradation. Bioseparation is the need of an hour since all the products we use needs to be purified before usage.
6. Which phase shows fixed velocity in process of chromatography in bioseparation engineering?
a) Carrier phase, mobile phase
b) Stationary phase
c) Mobile phase
d) Binding phase
Explanation: In chromatography, the mobile phase shows fixed velocity while passing through the column. There is a pulse of sample which is present in the molecules and is to be separated are injected into the column with the mobile phase.
7. Which method of bioseparation is based on size of the biological product?
a) Membrane separation, pervaporation
b) Electrophoresis, flotation
c) Filtration, membrane separation, centrifugation
d) Sedimentation, centrifugation, flotation
Explanation: The methods involved in bioseparation of biological products based on size are filtration, membrane separation, and centrifugation. Sedimentation, flotation are based on density. Pervaporation is based on volatility. Electrophoresis is based on electrostatic charges.
8. What are the two broad groups of bioseparation?
a) Colour and resolution
b) Resolution and productivity
c) Selectivity and colour
d) Productivity and colour
Explanation: The bioseparation are categorized into two broad groups Resolution and productivity. Resolution combines the high selectivity of the biological products and the productivity combines the high throughput of the biological products.
9. What is the example of liquid-liquid separations in bioseparation?
a) Fractionation of sub-cellular organelles
b) Separation of cells from culture medium
c) Separation of acetone and ethanol
d) Separation of serum albumin from other serum proteins
Explanation: Separation of acetone and ethanol is the example of liquid-liquid separation which has to be separated from liquid medium. It involves membrane distillation and pervaporation processes which is based on volatility of the particle to be separated.
10. Which bioseparation technique is used for separation of solid-liquid mixture through porous medium?
Explanation: The bioseparation technique is used for separation of solid-liquid mixture through porous medium is filtration, it requires the feed to be forced through a porous medium on which the solids substances are deposited or entrapped.
11. What are the important factors in the design of bioseparation?
a) usability, market, enzyme
b) purity, cost, market
c) usability, cost, chemical
d) purity, enzyme, chemical
Explanation: The factors important for the designing of bioseparations are purity, cost and market. All three are the vital criteria to fix before designing a setup for bioseparation. The cost of bioseparation depends on the product being purified and its market value.
12. What are the characteristics of best bioseparation setup?
a) Ensure high market value, instability, purity of the product
b) Ensure product degradation, partial purity, reproducibility
c) Ensure non-reproducibility, costly, instability of the product
d) Ensure product purity, stability, cost effectiveness
Explanation: The best bioseparation setup should ensure product purity, should provide stable products, the process must be cost effective and the products can be reproduced and should meet regulatory guidelines.
13. What are the most common examples of biologically derived products separated in bioseparation?
a) Animal tissue
c) Ethanol, Citric acid, Baker’s yeast
Explanation: The most commonly separated biologically derived products are solvents like ethanol; organic acids like citric acid; Cells like Baker’s yeast. These biological products are classified on the basis of their chemical nature. Ethanol has OH group, Citric acid has COOH and OH group, Baker’s yeast is an eukaryotic cell.
14. What is the bioseparation cost of bulk enzymes?
Explanation: The bioseparation cost of bulk enzymes is 40-65% of total cost. The approximate relative cost of production is 100% out of which 65% is required for the bioseparation.
15. Which of the following is a disadvantage of ultrafiltration in bioseparation?
a) Limited scalability
b) High cost
c) Risk of radiation exposure
d) Low selectivity
Explanation: Ultrafiltration may have limitations in terms of scalability, making it less suitable for large-scale bioseparation processes.
16. What is RIPP scheme in bioseparation?
a) Reuse, Isolation, Production, Polishing
b) Recovery, Isolation, Production, Packaging
c) Reuse, Isolation, Polishing, Packaging
d) Recovery, Isolation, Purification, Polishing
Explanation: The RIPP scheme stands for Recovery of the product from the fermentation broth followed by isolation of the product from the cells, followed by purification of the desired product and finally polishing of the product for final packaging. The scheme follows low-resolution techniques for recovery and isolation then followed by high resolution techniques. The high throughput and low resolution are used reduce the volume and concentration of the product. The high resolution and low throughput are used to produce pure and polished products.
Chapterwise Multiple Choice Questions on Bioseparation Technology
- Overview of Bioseparations Engineering
- Properties of Biological Material
- Mass Transfer
- Cell Disruption
- Membrane Based Bioseparation
- Miscellaneous Bioseparation Processes
1. MCQ on Overview of Bioseparations Engineering
The section contains multiple choice questions and answers covering key aspects of bioseparations engineering, including an overview, the economic importance of bioseparation, and various bioseparation processes and techniques.
2. Properties of Biological Material
The section contains Bioseparations questions and answers on biological material properties like size and molecular weight, diffusivity, osmotic pressure and sedimentation coefficient, solubility and partition coefficient, electrostatic charge, light absorption and fluorescence.
3. Bioseparation Technology MCQs on Mass Transfer
The section contains MCQs on solutes diffusion in dense and porous solid, convective mass transfer, molecular diffusion in liquid medium, inter phase and unsteady state mass transfer, mass transfer experimental determination, diffusivity measurement, mass transfer and diffusivity estimation.
4. Bioseparation Technology MCQ on Cell Disruption
The section contains Bioseparation Technology multiple choice questions and answers on cell disruption methods, covering topics such as bead mill, ultrasonic vibrations, organic solvents, osmotic shock, rotor-stator mill, French press, detergents, and enzymes.
5. Bioseparation Processes MCQ on Precipitation
The section contains questions and answers on precipitation factors, precipitate formation mechanism, precipitation using organic solvents and anti-chaotropic salts.
6. Bioseparation Processes Questions on Centrifugation
The section contains MCQs on centrifuge principles and types, ultracentrifugation, laboratory and preparative centrifuge.
7. Bioseparation Processes Multiple Choice Questions on Extraction
The section contains multiple choice questions and answers on extraction, extraction theory, solvent systems, aqueous two-phase extraction, batch and staged counter-current extraction, single-stage and cross-current continuous extractions using immiscible solvents, batch extraction using miscible solvents, as well as differential and supercritical fluid extractions.
8. Bioseparation Science MCQ on Adsorption
The section contains questions and answers on adsorption, adsorbents, separation mechanisms, adsorption isotherms, adsorption devices, adsorption processes limitations, batch and packed bed adsorption.
9. Bioseparation Science Questions on Chromatography
The section contains Bioseparation Science MCQs on chromatography basics, chromatography system and theory, chromatographic peak shape and yield, binary and hydrodynamic chromatography.
10. Bioseparation Engineering Questions on Filtration
The section contains multiple choice questions and answers on filtration basics and theory, filtration driving force, constant pressure and rate cake filtration, filtration efficiency improvement, filtration operation mode and equipment’s.
11. MCQ on Membrane Based Bioseparation
The section contains Bioseparation Engineering questions and answers on membrane separation basics, membrane processes classification, membrane equipment, ultrafiltration, microfiltration, dialysis, liquid membrane processes and membrane chromatography.
12. Miscellaneous Bioseparation Processes
The section contains Bioseparation Processes MCQs on electrophoresis, affinity ultrafiltration and field-flow fractionation.
Wish you the best in your endeavor to learn and master Bioseparation Technology!