This set of Bioseparation Science Multiple Choice Questions & Answers (MCQs) focuses on “Adsorbents”.
1. The adsorption process in bioseparation engineering utilizes particulate __________
a) adsorbate
b) adsorbent
c) absorbent
d) adhesive
View Answer
Explanation: The adsorption processes utilize particulate adsorbents. These adsorbent can be obtained from either natural sources or synthetic materials.
2. What is the common structure of the adsorbent used in the process of adsorption?
a) Microcrystalline
b) Crystalline
c) Amorphous
d) Microcrystalline or amorphous
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Explanation: The common structure of the adsorbent used in the process of adsorption is either amorphous or microcrystalline structure. These physical forms result in very high specific surface areas which specifies the surface area per unit amount of adsorbent.
3. What are the commonly used adsorbent in the process of adsorption in bioseparation processes?
a) Agarose, dextran, cellulose, silica
b) Cellulose, silica
c) Silica, resin
d) Resins, agarsoe
View Answer
Explanation: The commonly used adsorbent in the process of adsorption in bioseparation processes are cellulose based adsorbents, silica gel based adsorbents, synthetic resins, agarose based adsorbents and cross-linked dextran based adsorbents.
4. In which form can we use the particulate adsorbent?
a) Suspension
b) Packed bed
c) Suspension or packed bed
d) Suspension and packed bed
View Answer
Explanation: The particulate adsorbents can be used in the form of a suspension or in the form of a packed bed. There are possibilities of using expanded beds or fluidized bed form of suspension in the particulate adsorbent for the purpose of adsorption.
5. What are the applications of adsorption?
a) Protein purification
b) Nucleic acid purification
c) Antibiotic purification
d) Chromatography, dialysis, purification
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Explanation: The common applications of adsorption are purification of proteins, nucleic acid, antibiotic, analysis of biomedical samples, elution as well as pulse chromatography.
6. The adsorption energy determines the __________
a) pH
b) strength
c) solubility
d) stability
View Answer
Explanation: The adsorption energy determines the strength of the adsorbent to perform the process of adsorption with which any given molecule is adsorbed relative to other molecules within the system of adsorption.
7. The method of adsorption is limited to ____________
a) small molecules
b) large molecules
c) molecules of molecular weight less than 2000
d) molecules of molecular weight more than 2000
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Explanation: The adsorbed species are considered to form a monolayer on the surface of the adsorbent. The process limited to molecules with molecular weights less than 2000 because many of the surface sites may be of limited accessibility. The large molecules are difficult to elute because of the possibility of multiple sites of adsorptive interaction.
8. What is the concentration of the solute in the adsorption process in bioseparation engineering?
a) 1000ppm
b) 100ppm
c) 10ppm
d) 1ppm
View Answer
Explanation: The concentration of the solute in the adsorption technique is less than 1000 ppm. At concentration above 1000ppm the purification should be in terms of chromatographic methods of purification.
9. In the process of fermentation, the adsorption process is used for ___________
a) remove byproducts
b) remove products
c) recovery processes
d) purification of fermentation broth
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Explanation: Adsorption is utilized in fermentation recovery processes which includes removal of unwanted molecules, such as color bodies, from the solution of the fermentation product; adsorption of the fermentation product to increase its concentration when the product is eluted from the adsorption medium; adsorption of selectively the solutes in the fermentation produce stream to separate them by chromatography.
10. With the expansion of development of biotechnology, the advancement of adsorption has not increased.
a) False
b) True
View Answer
Explanation: With the expansion of the industries and with the development of the biotechnology applications in the last decade, the range of substances purified by adsorption has increased enormously.
Sanfoundry Global Education & Learning Series – Bioseparation Science.
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