This set of Bioseparation Technology Multiple Choice Questions & Answers (MCQs) focuses on “Bioseparations Engineering Overview”.
1. The major segment of biotechnology where research and development is vital is _______
a) Genetic engineering
b) Microbiology
c) Bioprocessing
d) Immunology
View Answer
Explanation: The growth of academia and industry is more in the area of Bioprocess engineering. It is one of the major segments of biotechnology where research and development is vital. It uses living cells to get a product of interest and it focuses on the fundamental theory of mass and energy conservation.
2. The products dealt under the major segment of bioprocessing are _________
a) Biochemicals
b) Biopharmaceuticals
c) Agrochemicals
d) Biochemicals, biopharmaceuticals, agrochemicals
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Explanation: The various biological products have been developed, approved and licensed under bioprocess engineering. The products manufactured under the segment of bioprocess include biochemicals, biopharmaceuticals, agrochemicals, food, and nutraceutical.
3. What is bioseparation engineering?
a) A systematic study of the scientific and engineering principle utilized for the large scale purification of biological products
b) A separation and purification segment which followed some form of biological reaction
c) A reaction and separation approach to study the biological compounds
d) A biological separation which involves engineering approach
View Answer
Explanation: Bioseparation engineering is the scientific study of engineering principles used in separation processes which are utilized for the large scale purification of biological products.
4. What is downstream processing?
a) The separation and purification segment of a bioprocess which followed some form of biological reaction
b) A systematic study of the scientific and engineering principle utilized for the large scale purification of biological products
c) A reaction and separation approach to study the biological compounds
d) A biological separation which involves engineering approach
View Answer
Explanation: Downstream processing is the separation as well as purification field of bioprocess which involves the utilization of biological reactions.
5. Purification of an antibiotic followed by microbial fermentation is an example of downstream processing.
a) True
b) False
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Explanation: Downstream processing is the separation and purification segment of a bioprocess which followed some form of biological reaction. It is the study of recycling the critical products and removing the impurity as well as by-products.
6. Why does the manufacturing of several types of biological products not involve in-vitro biological reactions?
a) The products are synthesized in vivo in their respective natural source
b) The products are simply recovered using appropriate techniques
c) The products are synthesized in vivo in their respective natural source and the products are simply recovered using appropriate techniques
d) The products cannot be simply recovered using appropriate techniques
View Answer
Explanation: The manufacturing of several types of biological products does not involve in-vitro biological reactions because the synthesis of products take place in vivo in their respective natural sources and recovery is performed using appropriate techniques.
7. Techniques involved in the in vivo manufacture and separation of products are _______
a) manufacture of plasma protein from blood
b) extraction of alkaloids from plants
c) extraction of enzymes from animal tissues
d) manufacture of plasma protein from blood, extraction of alkaloids from plants, extraction of enzymes from animal tissues
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Explanation: The example of appropriate techniques used in separation of biological products does not involve in-vitro biological reactions are manufacture of plasma protein from blood, extraction of alkaloids from plants, extraction of enzymes from animal tissues.
8. Bioprocessing is classified into different categories. They are _______
a) Reactive
b) Extractive
c) Non-reactive
d) Reactive and extractive
View Answer
Explanation: Bioprocessing is divided into two broad categories: reactive bioprocessing and extractive bioprocessing, among which reactive bioprocessing follows some form of biological reactions while the extractive bioprocessing involves only bioseparation.
9. What is the difference between reactive and extractive bioprocessing?
a) Reactive process follows some form of biological reactions while extractive almost entirely involves bioseparation
b) Reactive almost entirely involves bioseparation while extractive follows some form of biological reactions
c) Reactive follows non-biological reactions while extractive follows biological reactions
d) Reactive follows entire involvement of bioseparation while extractive follows some involvement of bioprocessing
View Answer
Explanation: Reactive processes have involvement of biological reactions such as fermentation like cultivation of microbial cells; cell culture involves cultivation of animal cells or plant cells and an enzymatic reaction while extractive involves only the bioseparation process.
10. In the context of reactive bioprocessing, upstream processing involves various steps.
a) Biocatalyst screening, enrichment, isolation and propagation
b) Cell manipulation by recombinant DNA technology or hybridoma technology
c) Media optimization and formulation
d) Biocatalyst screening, enrichment, isolation and propagation, cell manipulation by recombinant DNA technology or hybridoma technology and media optimization and formulation
View Answer
Explanation: The steps involved in upstream processing of reactive bioprocessing are biocatalyst screening, enrichment of media, isolation of cells and propagation of cells; cell manipulation through recombinant DNA technology or involvement of hybridoma technology; media optimization and media formulation.
11. The biological reactions involved in the reactive bioprocessing could be ________
a) Fermentation, cell culture, enzymatic reaction
b) Only Cell culture
c) Only fermentation
d) Both cell culture and enzymatic reaction
View Answer
Explanation: The biological reactions involved in the reactive bioprocessing could be fermentation such as cultivation of microbial cells; cell culture involves cultivation of animal cells or plant cells and an enzymatic reaction.
Sanfoundry Global Education & Learning Series – Bioseparation Technology.
To practice all areas of Bioseparation Technology, here is complete set of 1000+ Multiple Choice Questions and Answers.
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