This set of Bioseparation Technology Multiple Choice Questions & Answers (MCQs) focuses on “Bioseparation”.
1. What is separated in bioseparation?
a) Biologically derived products
b) Chemically derived products
c) Enzymatically derived products
d) Mechanically derived products
View Answer
Explanation: Bioseparation is the scientific study of engineering principles used in separation processes which are utilized for the large scale purification of biologically derived products.
2. The biological products are divided into some categories and are based on some parameters.
a) Chemical nature of the products
b) Applications of the products
c) Chemical nature and application of products
d) Biological nature of the product
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Explanation: The biologically derived products are divided into various categories out of which the two categories are based on the chemical nature of the product and the applications of the product. Chemical nature of products includes enzyme activity, amino acid sequence and so on. Application of products includes industrial production process, production of vaccines, and production of commodity chemicals.
3. What are the most common examples of biologically derived products separated in bioseparation?
a) Ethanol, Citric acid, Baker’s yeast
b) Penicillin
c) Animal tissue
d) Cytokines
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Explanation: The most commonly separated biologically derived products are solvents like ethanol; organic acids like citric acid; Cells like Baker’s yeast. These biological products are classified on the basis of their chemical nature. Ethanol has OH group, Citric acid has COOH and OH group, Baker’s yeast is an eukaryotic cell.
4. Bioproducts can be broadly classified into the following categories: small molecules, large molecules and particulate products.
a) True
b) False
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Explanation: Bioproducts are widely classified as small molecules which consists of fine chemicals, antibiotics, hormones, amino acids, and vitamins; large molecules which consists of proteins, polysaccharides, and nucleic acids; and particulate products which consists of cells, spores, liposomes, and subcellular particles or organelles.
5. What are the broad division of biological products corresponds to another broad category of separation process for purifying the biological products?
a) Extraction, Adsorption, Sedimentation
b) Sedimentation, filtration, concentration
c) Filtration, adsorption, drying
d) Crystallization, drying, filtration
View Answer
Explanation: The broad division of biological products corresponds to another broad category of separation process for purifying the biological products are extraction, adsorption and sedimentation. The small molecules cannot be separated through sedimentation, but they can be separated by extraction; most of the large molecules cannot withstand the conditions of extraction, but they are highly adsorptive and particulate products can be collected by sedimentation.
6. What are the examples of small molecules and their molecular weight?
a) Amino acid, 60-200 Da
b) Proteins, 103 Da
c) Nucleic acid, 106 Da
d) Sugar, 1000 Da
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Explanation: The amino acids are one of the small molecules having molecular weight 60-200 Da and are naturally occurring compounds. It is made up of basic amino group, acidic carboxyl group and organic R group and the key elements includes C, H, O, N.
7. What are the examples of large molecules with their molecular weight?
a) Amino acid, 60-200 Da
b) Proteins, 103 – 106 Da
c) Nucleic acid, 1020 Da
d) Sugar, 1000 Da
View Answer
Explanation: The proteins are one of the large molecules having molecular weight of 103 – 106 Da and are commonly known as macromolecules. These are complex molecules used as an essential source of energy and for building the tissues in the body.
8. What are the examples of particulate matters?
a) Amino acid
b) Antibiotics
c) Polysaccharides
d) Ribosome
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Explanation: Ribosome is one of the biologically derived particulate matters which are separated through bioseparation method of sedimentation. It is a complex matter which is the site for synthesis of proteins. It helps in attachment of the amino acids and mRNA to tRNA.
9. Sugar is an example of which biologically derived product and what is the range of its molecular weight?
a) Small molecules, 200-600 Da
b) Large molecules, 200-600 Da
c) Small molecules, 20-60 Da
d) Particulate matter, 20-60 Da
View Answer
Explanation: Sugar is a small molecule having molecular weight 200-600 Da. It is a monomer of polysaccharides and is naturally occurring biological products which can be separated using extraction method of bioseparation.
10. Animal cells are considered large molecules in the category of biologically derived products.
a) True
b) False
View Answer
Explanation: Animal cells are biologically derived particulate matter which can be separated through sedimentation or filtration method of bioseparation. It has plasma membrane, centrioles, lysosomes which make the cell more complex and large.
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