This set of Bioseparation Technology Multiple Choice Questions & Answers (MCQs) focuses on “Cell Disruption Methods”.
1. Which organic solvent is used for disruption of fungal cell wall?
a) Toluene
b) Acetone
c) Acetic acid
d) Water
View Answer
Explanation: Toluene is used to disrupt the fungal cell wall. Toluene is an organic chemical which is also known as methylbenzene is insoluble in water. The fungal cell wall is tough and it needs heavy chemicals to disrupt the complexity of the cell wall.
2. Which organic solvent is used to disrupt plasma membranes of bacterial cell?
a) Toluene
b) Acetone
c) Acetic acid
d) Water
View Answer
Explanation: Acetone breaks the cell membrane by solubilising the phospholipids of the membrane and denatures the protein of the membrane.
3. What is the limitation of using organic solvents for cell disruption?
a) Addition in the cell suspension
b) Removal of cell suspension
c) Removal of the organic solvent from the suspension
d) Addition of the organic solvent from the suspension
View Answer
Explanation: Removal of the organic solvent from the suspension is a limitation of using organic solvent for cell disruption. The removal of organic solvent as well as cell debris from the product is difficult depending on the type of solvent used.
4. On what basis organic solvents can be removed from the cell suspension?
a) Solubility
b) Ignition rate
c) Temperature
d) Volatility
View Answer
Explanation: The organic solvents can be removed from the cell suspension on the basis of volatility of the organic solvent used. The organic solvents can be separated depending on which type of solvent is used, some solvents have tendency to evaporate at certain temperature.
5. How is osmotic pressure generated in the solution?
a) Difference in solute concentration
b) Same solute concentration
c) Salting in
d) Salting out
View Answer
Explanation: Osmotic pressure is generated due to the difference in solute concentration across the membrane which is semi permeable. The plasma membrane is a semi permeable membrane and transfer of cell from higher concentration to lower concentration will lead to development of osmotic pressure.
6. Transfer of cell from isotonic solution to hypotonic solution results into ___________
a) aggregation of cells
b) rapid influx of hypotonic solution in the cell
c) precipitation of solution
d) precipitation of cells
View Answer
Explanation: The cells when transferred from isotonic solution to hypotonic solution results into rapid influx of water i.e. hypotonic solution in the cell and it will expand the cell volume and finally ruptures the cell.
7. Which type of cells is ruptured using osmotic pressure?
a) Yeast cells
b) Plant cell
c) Mammalian cells
d) Fungal cell
View Answer
Explanation: Mammalian cells are disrupted using osmotic pressure. Plant cells are lysed using bead mills. Fungal cells are ruptured using organic solvent. Yeast cells are ruptured using ultrasonic vibrations.
8. Osmotic shock is used to remove ___________
a) Cytoplasm
b) Nucleus
c) Cytosol
d) Periplasmic substance
View Answer
Explanation: Periplasmic materials like proteins are removed by giving osmotic shock treatment, from the cell without using any physical method of cell disruption. Most of the proteins and other macromolecules are secreted in periplasmic space and these need to b removed for cell disruption and further purification of desired product with the cell.
9. What will happen to the cells when they are transferred to hypotonic solution?
a) Cells imbibe water
b) Cells precipitate
c) Cells aggregate
d) Cells coagulate
View Answer
Explanation: Cells imbibe water through the process of osmosis when it is transferred to hypotonic solution and the volume of water in the cell membrane increases and the material in cell wall does not expand which expels in the liquid.
10. Red blood cells when suddenly introduced into water will not hemolyse the cell.
a) True
b) False
View Answer
Explanation: The red blood cells hemolyses when they are introduced to water and hence the cell disrupts which helps in releasing of haemoglobin in the liquid medium.
Sanfoundry Global Education & Learning Series – Bioseparation Technology.
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