This set of Bioseparation Processes Multiple Choice Questions & Answers (MCQs) focuses on “Affinity Ultrafiltration”.
1. In ____________ separation process, the problem is diffusion in the adsorption and the elution steps.
a) affinity chromatography
b) column chromatography
c) affinity ultrafiltration
d) dialysis
View Answer
Explanation: In column chromatography process of separation, the problem associated is the prevalence of the diffusion process in the process of adsorption and the step involved at the end i.e. elution steps.
2. The other problem associated with the column chromatography is the created around the binding sites.
a) True
b) False
View Answer
Explanation: The other problem associated with the column chromatography is the steric hindrance created in the confined spaces around the binding sites and facing difficulty in step of scale up.
3. The problems associated with the column chromatography can be overcome using ___________
a) affinity ultrafiltration
b) column chromatography
c) affinity chromatography
d) dialysis
View Answer
Explanation: The problems associated with the column chromatography can be overcome using one of the way in which affinity separation in carried out in an ultrafiltration device and the final process of separation is termed as affinity ultrafiltration.
4. The steps involved in affinity ultrafiltration are adsorption and washing.
a) True
b) False
View Answer
Explanation: There are three steps involved in affinity ultrafiltration and the first step is adsorption of the molecules to the ligand followed by washing off the impurities and finally elution of the target molecule free from the impurities.
5. The affinity ligand is _________ in nature in the process of affinity ultrafiltration.
a) micro molecular
b) selective
c) permeable
d) macromolecular
View Answer
Explanation: The affinity ligand is macromolecular in nature in the process of affinity ultrafiltration that means it can retain in the solution by a suitable membrane used for ultrafiltration.
6. The ultrafiltration membrane are selected on the basis of __________
a) retaining capacity
b) pressure drop
c) pH difference
d) temperature variation
View Answer
Explanation: The ultrafiltration membrane are selected on the basis of total retaining capacity of the macro-ligand in the solution mixture having molecules as well as the impurities.
7. The ultrafiltration membrane allows ________ passage of the molecule.
a) limited
b) unlimited
c) difficult
d) easy
View Answer
Explanation: The ultrafiltration membrane allows easy passage of the molecule i.e. the target which is being considered as the solute along with the impurities present in the molecule.
8. In which step of the process of affinity ultrafiltration, the mixture which is to be separated needs to be introduced?
a) The mixture will not be introduced
b) Elution step
c) Binding step
d) Washing step
View Answer
Explanation: In the binding step of the process of affinity ultrafiltration, the mixture which is to be separated needs to be introduced and the introduction will be done in the membrane module.
9. In the affinity ultrafiltration, the introduction of the mixture in the membrane module can be done _________
a) either as pulse or in the form of a step
b) neither as pulse nor in the form of a step
c) in the form of step
d) as pulse
View Answer
Explanation: In the affinity ultrafiltration, the introduction of the mixture in the membrane module can be done either as pulse or in the form of a step that can be taken into consideration as an initial step of the process of separation.
10. _________ are washed off the system of affinity ultrafiltration in __________ step.
a) Washing, impurities
b) Unbound impurities, washing
c) Binding, molecules
d) Molecules, washing
View Answer
Explanation: The unbound impurities are washed off the membrane used in the separation process in the affinity ultrafiltration and this is done in the washing step, followed by the elution of the target solute which is to be done in a specific method.
Sanfoundry Global Education & Learning Series – Bioseparation Processes.
To practice all areas of Bioseparation Processes, here is complete set of 1000+ Multiple Choice Questions and Answers.
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