This set of Bioseparation Technology Multiple Choice Questions & Answers (MCQs) focuses on “Biological Material Properties – Size and Molecular Weight”.
1. What is the SI unit of molecular weight?
a) Kg/mol
b) Pascal
c) Kg/m3
d) Cm/s
View Answer
Explanation: Kg/mol is the SI unit of molecular weight. Pascal is the SI unit of Pressure. Kg/m3 is the SI unit of density. Cm/s is the SI unit of velocity.
2. The molecular weight of nucleic acids is expressed in terms of __________
a) avogadro’s number
b) number of base pair of nucleotide
c) turns/base pair
d) helical coil
View Answer
Explanation: The molecular weight of nucleic acids like plasmids and DNA are expressed in terms of number of base pair of nucleotide. DNA is a polymer of nucleotides and 1 base pair of nucleotide is equal to 660kg/mole.
3. Which process of bioseparation requires linking of molecular weight of product with size of the product for separation?
a) Sedimentation, precipitation, centrifugation
b) Gel-filtration, precipitation, centrifugation
c) Gel-filtration, hydrodynamic chromatography, membrane separation
d) Sedimentation, membrane separation, chromatography
View Answer
Explanation: Gel-filtration chromatography also called size-exclusion chromatography is used in separation molecules on the basis of size and also used to estimate the molecular weight of proteins. Hydrodynamic chromatography is a variant of size exclusion chromatography. Membrane separation is utilized in separation of particles based on the size of the product along with the weight of the same product.
4. Which properties of the product are influenced by the molecular weight of the product?
a) Density, charges
b) Solubility, mobility in electric field
c) Density, diffusivity
d) Diffusivity, mobility in electric field
View Answer
Explanation: The diffusivity is influenced by the molecular weight, the heavier the molecule less will be its movement and so it will diffuse slowly. The mobility in electric field is also influenced by the molecular weight of the product, the mobility of DNA in electric field increases when molecular weight of product increases.
5. For which bioseparation process, the molecular weight is the indirect basis of separation?
a) Ultrafiltration, electrophoresis
b) Sedimentation, precipitation
c) Centrifugation, ultra filtration
d) Electrophoresis, sedimentation
View Answer
Explanation: The rate of migration of a molecule in an electrophoresis matrix depends on the molecular weight of the molecule therefore the electrophoresis is indirectly influenced by the molecular weight for separation. The movement of particles through a semi-permeable membrane in Ultrafiltration depends on the molecular weight of the particle therefore the ultrafiltration is indirectly influenced by the molecular weight of the particle for separation.
6. The size of particles and the molecular weight of substances can be polydispersed.
a) False
b) True
View Answer
Explanation: The polydispersed feature of substances refers to the molecular weight distribution of the particles. The substances have various ranges in the molecular weights as well as have particles of various sizes.
7. What is the example of substances which have polydispersed molecular weight?
a) Glucose, Fructose
b) Galactose, Mannose
c) Dextran, starch
d) Ribose, sodium chloride
View Answer
Explanation: Dextran and starch are polysaccharides having very high molecular weight range. Dextran chain length varies from 3 to 2000KDa and it is a branched poly-α-D-glucosidase with glucosidic bonds from C-1 -> C-6”. Starch is a double branched molecule having linear and 25% of helical amylase as well as 75% branched amylopectin.
8. Paucidispersed is the special case of polydispersed system in any molecule.
a) True
b) False
View Answer
Explanation: The molecules with paucidispersed system have distributed molecular weights in the multiples of various small molecular weights, which is different than polydispersed system that’s why it is considered to be the special case of polydispersed system.
9. What is the example of paucidispersed system?
a) Dextran
b) Galactose
c) Cellulose
d) Immunoglobulin G in solution
View Answer
Explanation: Immunoglobulin exists in the distributed molecular weights with multiple small molecular weights. It occurs as a monomer, having dimers as well as some amount of tetramers and trimers.
10. What is the basis of determining the molecular weights of smaller molecules?
a) Structural formula
b) Size
c) Density
d) Charges
View Answer
Explanation: The small molecules do not require any tedious methods for estimating the molecular weights, simply by using the molecular formula weight can be calculated. For example, molecular weight of sodium chloride can be calculated using molecular formula NaCl. Molecular weight of sodium (Na) = 23 kg/mole, mol. Wt. of Chlorine (Cl) = 35.5 kg/mole, therefore by combining both the weights the final weight of NaCl = 23 + 35.5 = 58.5 Kg/mole.
Sanfoundry Global Education & Learning Series – Bioseparation Technology.
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