This set of Bioseparation Science Multiple Choice Questions & Answers (MCQs) focuses on “Chromatography”.
1. Which technique relies on the dynamic distribution of molecules for separation?
a) Chromatography
b) Precipitation
c) Electrophoresis
d) Membrane separation
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Explanation: Chromatography is the technique which requires a solute fractionation for relying on the dynamic distribution of molecules for the separation between two phases, binding or a stationary phase and a carrier phase or mobile phase.
2. Which phase is particulate in nature in terms of chromatography?
a) Carrier phase
b) Binding phase
c) Stationary phase, binding phase
d) Mobile phase
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Explanation: In chromatography, the stationary phase is particulate in nature because of the particles since, particles are packed within the column in the form of a packed bed and they do not show any movement.
3. Which phase shows fixed velocity in process of chromatography in bioseparation engineering?
a) Binding phase
b) Carrier phase, mobile phase
c) Stationary phase
d) Mobile phase
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Explanation: In chromatography, the mobile phase shows fixed velocity while passing through the column. There is a pulse of sample which is present in the molecules and is to be separated are injected into the column with the mobile phase.
4. The velocity of movement of molecules through the column depends on the _____________
a) Mobile phase
b) Stationary phase
c) Molecules
d) Column
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Explanation: The velocity of movement of molecules through the column depends on the stationary phase. A molecule which has no interaction with the stationary phase, shows same velocity as that of the mobile phase.
5. The molecules which does not interact with the stationary phase have less extent of interaction.
a) False
b) True
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Explanation: The molecules which interact with the stationary phase have higher extent of interaction than the mobile phase and have slow velocity.
6. What is pulse chromatography?
a) Less extent of interaction
b) No interaction with stationary phase
c) Greater extent of interaction
d) High velocity
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Explanation: The molecules that have interaction with the stationary phase and have the greater extent of interaction showing slower velocity is known as pulse chromatography and is also considered as mode of chromatographic separation.
7. Chromatography is used in separation of _____________
a) proteins
b) metals
c) salts
d) plastic
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Explanation: The chromatographic technique is used for the separation of various substances which includes proteins, lipids, hormones, carbohydrates, nucleic acids, antibiotics and so on.
8. The process of chromatography is termed as analytical when _____________
a) used in separation of products
b) used in separation of process
c) used in separation of molecules
d) separation of complex mixtures
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Explanation: The process of chromatography is termed as analytical when the method is used in separation as well as analysis of mixtures having complex molecules and compositions.
9. The process of chromatography is termed as preparative when _____________
a) used in separation of products
b) used in separation of process
c) used in separation of molecules
d) separation of complex mixtures
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Explanation: The process of chromatography is termed as preparative when the method is used in separation of molecules having use in the process of manufacturing.
10. The major applications of chromatography are ____________
a) production
b) analysis
c) production, diagnosis
d) production, monitoring, diagnosis, analysis
View Answer
Explanation: The major applications of chromatography are analysis of environmental related issues, biomedical related issues as well as biopharmaceuticals; production of food and pharmaceuticals as well as biopharmaceuticals; monitoring of process; diagnostic related issues.
Sanfoundry Global Education & Learning Series – Bioseparation Science.
To practice all areas of Bioseparation Science, here is complete set of 1000+ Multiple Choice Questions and Answers.
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