# Bioseparation Science Questions and Answers – Adsorption Devices

This set of Bioseparation Science Multiple Choice Questions & Answers (MCQs) focuses on “Adsorption Devices”.

1. What is the length of the equilibrium zone when a protein is being adsorbed in a 1.5 m long packed bed containing DEAE-cellulose particles. Also find out the length of the adsorption zone at breakthrough. What is utilized bed capacity of the fraction at breakthrough? Given: Breakthrough time is 8 hours and exhaustion times is 10 hours.
a) 1.125m, 0.375m, 0.875
b) 1.125m, 0.875m, 0.375
c) 0.875m, 1.125m, 0.375
d) 0.375m, 1.125m, 0.875
View Answer

Answer: a
Explanation: The length of the equilibrium zone is given by leq = l(1 – $$\frac{t” – t’}{t’})$$ ∴ leq = 1.5(1 – $$\frac{10 – 8 }{8})$$ = 1.125m and the lenght of the adsorption zone is given by lad = $$\frac{l(t” – t’)}{t’}$$ ∴ lad = $$\frac{1.5(10 – 8)}{8}$$ = 0.375m. The fraction of bed capacity is given by θ = (1 – $$\frac{t” – t’}{t’})$$ ∴ θ = 1 – $$\frac{10 – 8}{16}$$ = 0.875.

2. What will be the exhaustion time of an antibody which is being adsorbed by an affinity adsorbent contained in a 5 cm long packed bed? Given: Breakthrough time to be 30 minutes and 80% of the binding capacity was utilized.
a) 35min
b) 36min
c) 40min
d) 42min
View Answer

Answer: d
Explanation: Since the breakthrough is 80%, then the fraction of bed capacity i.e. θ = 0.8 Hence, θ = 1 – $$\frac{exhaustion\, time – 8}{16}$$ = 0.875. Therefore, Exhaustion time is 42min.

3. What is the significance of the given diagram?

a) Membrane adsorber
b) Fluidized bed adsorber
c) Continuous flow adsorber
d) Batch flow adsorber
View Answer

Answer: b
Explanation: In the fluidized bed adsorber the feed entering from the bottom passes through the fluidized bed which contains the adsorbents and finally the effluents are collected from the top at the end of the process of adsorption.
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4. What is the significance of the given diagram?

a) Membrane adsorber
b) Fluidized bed adsorber
c) Continuous flow adsorber
d) Batch flow adsorber
View Answer

Answer: a
Explanation: In the membrane adsorber, the feed passes through the stack of porus membrane which allows the feed to be adsorbed and then the final effluents are collected from the top of the adsorber.

5. What are the problems associated with packed bed adorber?
a) High pressure drop
b) High pumping cost, high pressure drop
c) Radial dispersion
d) High pressure drop, Radial dispersion
View Answer

Answer: d
Explanation: The problems associated with the packed bed adsorbers are high pressure drop across the beds which actually results into high pressure drop, it is essential to use low flow rates in the adsorber of packed beds and it undergoes radial dispersion.

6. The inability to handle the feed with the particular material is the drawback associated with the _____________
a) fluidized bed adsorber
b) membrane adsorber
c) packed bed adsrober
d) expanded bed adsorber
View Answer

Answer: c
Explanation: The inability of handling the fed in the packed bed adsorber leads to overflow of feed and the flow rates causes the improper adsorption of the material causing the loss of product and improper adsorption.

7. The feed is pumped into the column from the bottom in ____________
a) unexpanded bed
b) fluidized bed
c) membrane bed
d) packed bed
View Answer

Answer: b
Explanation: The feed is pumped into the column from the bottom in fluidized bed adsorber, at a velocity which is sufficient to fluidize the adsorbent bed and to complete the process of adsorption.
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8. The problems of packed bed can be overcome using ____________
a) expanded system
b) unexpanded system
c) fluidized bed, membrane adsorber
d) membrane bed
View Answer

Answer: c
Explanation: The problems associated with packed bed adsorber for the process of adsorption could be overcome using fluidized bed or expanded bed adsorbers like membrane bed adsorber and in fluidized or expanded bed systems, the adsorbent is loosely packed within a column with some space on the top.

9. The stacks are used for adsorption in ____________
a) expanded system
b) unexpanded system
c) fluidized bed, membrane adsorber
d) membrane bed
View Answer

Answer: d
Explanation: The adsorber which uses stack for the process of adsorption in bioseparation engineering is Membrane adsorbers and the use stacks of adsorptive membranes is helpful in overcoming the issues related to the packed bed adsorber.
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10. For a packed bed adsorber, the breakthrough is given by ____________
a) feed flow rate
b) effluent flow rate
c) composition of the bed
d) molecules in the adsorber
View Answer

Answer: a
Explanation: Feed flow rate is the major factor related to the dependency of the breakthrough curve in the packed bed adsorber, for those molecules which have low diffusivities, they undergo lower flow rates which results in sharper breakthroughs.

Sanfoundry Global Education & Learning Series – Bioseparation Science.

To practice all areas of Bioseparation Science, here is complete set of 1000+ Multiple Choice Questions and Answers.

If you find a mistake in question / option / answer, kindly take a screenshot and email to [email protected]

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