This set of Bioseparation Engineering Multiple Choice Questions & Answers (MCQs) focuses on “Membrane Chromatography”.
1. The process of ____________ separations are basically carried out using packed beds.
a) filtration
b) sedimentation
c) chromatographic
d) packed bed
View Answer
Explanation: The processes of adsorption and the chromatographic separations are conventionally passed through packed beds, though packed beds have several limitations in functioning.
2. The major limitation of packed bed in membrane separation is ___________
a) high pressure drop
b) filtration
c) low pressure drop
d) expansion of bed
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Explanation: The major limitation includes high drop of pressure across the beds, enhancement of the pressure drop meanwhile the operation proceeds, binding of packed beds by the macromolecules, intra-particle dependence for the transport of solute, issues related to scale-up.
3. The use of _________ resolves the effects caused by limitations of using packed beds.
a) packed bed
b) expanded bed
c) conventional bed
d) pressure drop
View Answer
Explanation: The expanded beds as well as the fluidized bed can be sued in the solving the demerits of using the packed bed membrane chromatography method for the bioseparation process.
4. What is the alternative approach for the use of packed bed?
a) Micro-porous membrane
b) Increase pressure drop
c) Scale up
d) Micro-porous or macro-porous membrane
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Explanation: The alternative approach to the use of packed beds are not just use of fluidized beds and expanded bed for the purpose of adsorption, there has been use of stacks of synthetic micro-porous as well as macro-porous membranes as adsorptive media or as chromatographic media.
5. In the process of ___________ packed bed adsorption, solute transport depends on diffusion.
a) conventional
b) non-conventional
c) chromatographic
d) adsorptive
View Answer
Explanation: In process of conventional packed bed adsorption, predominantly in methods using soft porous media for the solutes transport to their binding sites heavily depends on diffusion and is hence slow.
6. The transport of solute in membrane adsorption takes place by _________
a) convention
b) condensation
c) convection
d) filtration
View Answer
Explanation: The transport of solute in membrane adsorption takes place by the process of convection and hence the process of separation is faster.
7. The major feature of using a chromatographic membrane is __________
a) very Low bed height to diameter ration
b) very High bed height to diameter ratio
c) low bed width to bed height ratio
d) high bed width to bed height ratio
View Answer
Explanation: The major feature of using a chromatographic membrane is the very low bed height to diameter ratio which means that there is less requirements of the pressure drop.
8. The basic advantage of using chromatographic membrane is _________
a) high pressure drop
b) low process time
c) high process requirement
d) low flow rate
View Answer
Explanation: The basic advantage of using chromatographic membrane includes low process time along with low requirement of process liquid, very high flow rates, low pressure drop, less column blinding, easy way of scale -up, less problem associated with re-validation.
9. The other separation mechanism that has been utilized by membrane chromatography is ________
a) filtration
b) sedimentation
c) affinity binding
d) adsorption
View Answer
Explanation: The affinity binding process is one of the separation mechanism that is used by membrane chromatography along with the ion-exchange interaction as well as hydrophobic interaction.
10. The __________ type membrane adsorbers are commonly used for laboratory scale microfiltration.
a) flat sheet
b) packed bed
c) infused bed
d) particulate adsorbent
View Answer
Explanation: There are three types of membrane adsorbers depending on the membrane geometry and they are flat sheet, radial flow and hollow fibre. Out of these flat sheet type membrane adsorbers look like syringe type filters and is commonly used for laboratory scale microfiltration, having commercial availability such adsorbers are typically operated in the bind and elute mode.
Sanfoundry Global Education & Learning Series – Bioseparation Engineering.
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