This set of Bioseparation Technology Multiple Choice Questions & Answers (MCQs) focuses on “Basis of Separation in Bioseparation Processes – Set 2”.
1. What are the physical forms of liquid medium separated in bioseparation?
a) Particle-particle, particle-particle in liquid medium, solute-solute
b) Particle-solute in liquid medium, solute-solute, liquid-solid
c) Particle-particle in liquid medium, Particle-solute in liquid medium, Solute-solute in liquid medium
d) Solute-solute, particle-solid, particle-particle
View Answer
Explanation: The physical forms of liquid medium separated in bioseparation are particle-particle in liquid medium, Particle-solute in liquid medium, Solute-solute in liquid medium. Particle-particle, solute-solute, particle-solid are the physical forms of non-liquid medium separated in bioseparation.
2. What are the examples of particle-liquid separation?
a) Separation of cells from culture medium, separation of blood cells from plasma, removal of bacteria from protein solution
b) Fractionation of sub-cellular organelles, separation of plasmid DNA from chromosomal DNA, separation of mature cells from young cells
c) Separation of dissolved antibiotics from cell debris and cells in fermentation broth
d) Removal of dissolved impurities from liquid products, solvent exchange
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Explanation: The examples of particle-liquid separations are separation of blood cells from plasma, the separation of cells from culture medium and removal of bacteria from protein solution. Fractionation of sub-cellular organelles, separation of plasmid DNA from chromosomal DNA, separation of mature cells from young cells is the example of particle-particle in liquid medium. Separation of dissolved antibiotics from cell debris and cells in fermentation broth is the example of particle-solute separation in liquid medium. Removal of dissolved impurities from liquid products, solvent exchange is the examples of solute-solvent separations in bioseparation.
3. What are examples of particle-particle separation in liquid medium?
a) Separation of cells from culture medium, separation of blood cells from plasma, removal of bacteria from protein solution
b) Fractionation of sub-cellular organelles, separation of plasmid DNA from chromosomal DNA, separation of mature cells from young cells
c) Separation of dissolved antibiotics from cell debris and cells in fermentation broth
d) Removal of dissolved impurities from liquid products, solvent exchange
View Answer
Explanation: The examples of particle-particle separation in liquid medium are separation of plasmid DNA from chromosomal DNA, fractionation of sub-cellular organelles and separation of mature cells from young cells. It includes processes like sedimentation, filtration and floatation. The separation process is based on size of the particle.
4. What is example of particle-solute separation in liquid medium?
a) Separation of cells from culture medium, separation of blood cells from plasma, removal of bacteria from protein solution
b) Fractionation of sub-cellular organelles, separation of plasmid DNA from chromosomal DNA, separation of mature cells from young cells
c) Separation of dissolved antibiotics from cell debris and cells in fermentation broth
d) Removal of dissolved impurities from liquid products, solvent exchange
View Answer
Explanation: Separation of dissolved antibiotics from cell debris and cells in fermentation broth is the example of particle-solute separation in liquid medium. It involves processes like distillation, condensation and adsorption. The separation processes are based on volatility as well as polarity.
5. What are the examples of solute-solvent separation in bioseparation?
a) Separation of cells from culture medium, separation of blood cells from plasma, removal of bacteria from protein solution
b) Fractionation of sub-cellular organelles, separation of plasmid DNA from chromosomal DNA, separation of mature cells from young cells
c) Separation of dissolved antibiotics from cell debris and cells in fermentation broth
d) Removal of dissolved impurities from liquid products, solvent exchange
View Answer
Explanation: Removal of dissolved impurities from liquid products, solvent exchange is the examples of solute-solvent separations in bioseparation. The separation processes include membrane separation, distillation. These processes are based on diffusivity of the particle to be separated.
6. What are the examples of solute-solute separations in liquid medium?
a) Separation of cells from culture medium
b) Fractionation of sub-cellular organelles
c) Separation of serum albumin from other serum proteins
d) Separation of acetone and ethanol
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Explanation: Separation of serum albumin from other serum proteins is the example of solute-solute separations in liquid medium. The separation processes include adsorption which is based on polarity and electrostatic charges.
7. What is the example of liquid-liquid separations in bioseparation?
a) Separation of cells from culture medium
b) Fractionation of sub-cellular organelles
c) Separation of serum albumin from other serum proteins
d) Separation of acetone and ethanol
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Explanation: Separation of acetone and ethanol is the example of liquid-liquid separation which has to be separated from liquid medium. It involves membrane distillation and pervaporation processes which is based on volatility of the particle to be separated.
8. Which method of separation follows the principle of forcing the suspension through a porous medium in which the particles are retained and the liquid is allowed to follow?
a) Filtration and membrane separation
b) Zonal centrifugation
c) Solvent exchange
d) Extraction
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Explanation: Filtration and membrane separation follows the principle of forcing the suspension through a porous medium in which the particles are retained and the liquid is allowed to follow. Zonal centrifugation follows the principle of subjecting the suspension to induced gravitational force. Solvent exchange follows removal of dissolved impurities from liquid. Extraction follows separation of immiscible liquid in which the target has high solubility.
9. Which method of separation follows the principle of subjecting the suspension to induced gravitational force?
a) Filtration and membrane separation
b) Zonal centrifugation and sedimentation
c) Solvent exchange
d) Adsorption
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Explanation: Zonal centrifugation and sedimentation follows the principle of subjecting the suspension to induced gravitational force. Centrifugation is the separation of particles under centrifugal force. Sedimentation is the separation of unstable solids from the suspension under gravitational force.
10. Which method of separation is followed when the suspended particles are denser than the liquid medium will settle and will form a zone with high particle concentration?
a) Filtration and membrane separation
b) Solvent exchange
c) Adsorption
d) Zonal centrifugation
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Explanation: Zonal centrifugation is used when the suspended particles are denser than the liquid medium which they are suspended; the particles will settle and will form a zone with high particle concentration.
11. Which method of separation is followed when the suspended particles are lighter than the liquid medium in which particles are suspended and it will float by concentrating at the top of the container?
a) Filtration and membrane separation
b) Floatation
c) Extraction
d) Zonal centrifugation
View Answer
Explanation: Floatation is used for separation when the suspended particles are lighter than the liquid medium in which particles are suspended and it will float by concentrating at the top of the container. Floatation is the separation of aqueous suspensions and its concentration.
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