This set of Bioseparation Science Multiple Choice Questions & Answers (MCQs) focuses on “Adsorption”.
1. What is referred as adsorption, in bioseparation engineering?
a) Binding of molecules
b) Binding of molecules on surface of solid
c) Binding of solid to liquid
d) Binding
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Explanation: The binding of molecules on solid surface are referred as adsorption in which the transfer of dissolved solutes from a liquid phase is involved to the surface of solid phase.
2. Adsorption is the penetration of substances into the porous structure within the solid material.
a) False
b) True
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Explanation: The process of absorption refers to the penetration of substances into the porous structure within the solid material while the process of adsorption refers to binding of molecules on solid surface.
3. What is the major application of the process of adsorption in bioseparation engineering?
a) Separation
b) Separation of molecules
c) Separation of molecules from a complex mixture of molecules
d) Penetration of substance in solid surface
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Explanation: The separation of solute from the solvent i.e. separation of molecules from a complex mixture of molecules is the major application of adsorption in bioseparation engineering.
4. The molecule which binds on the adsorbent is referred as adsorbent.
a) True
b) False
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Explanation: The molecule which binds on the adsorbent is called adsorbate. The solute binding takes place usually on the specific locations on the surface of the adsorbent.
5. The separation of molecules from the mixture of molecules are achieved by the contacting of solution to ___________
a) adsorbate
b) absorbate
c) absorbent
d) adsorbent
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Explanation: The adsorbent is the solid material which helps in separation of molecules from the complex mixture of molecules through creating contact of the solution with the solid.
6. The solute binds on specific location on the surface of the adsorbent and is referred to as _________
a) binding site
b) ligand
c) ligand or binding site
d) site
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Explanation: The binding site or the ligand are the specific location where the solutes bind on the surface of the adsorbent and the term is specific to the molecular entity which is grafted to the surface of the solid to prepare the adsorbent.
7. Which is the selective form of adsorption in bioseparation engineering?
a) Affinity binding
b) Cooperative binding
c) Hill binding
d) Binding
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Explanation: The affinity binding is the selective form of adsorption where highly specific interactions between molecules and their binding sites are utilized. In an adsorption based separation process, the bound material subsequently needs to be released, i.e. the binding process should be reversible in nature.
8. The release of adsorbed material from the adsorbent is referred to as ________
a) absorption
b) absorption
c) desorption
d) adsorbate
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Explanation: The release of adsorbed material from an adsorbent is referred to as desorption. The process of desorption is favored at conditions opposite to those that favor adsorption.
9. Adsorption is influenced by ____________
a) molecular weight, shape of ligand, polarity
b) shape of ligand, polarity, charge
c) polarity
d) charge
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Explanation: The process of adsorption is influenced by molecular weight, size and shape of solute, shape of the binding site or ligand, polarity of the molecules and the adsorbent, electrostatic charge on the molecule and on the adsorbent.
10. What are the applications of adsorption?
a) Ability to recover solutes from the dilute solution
b) Ability to recover solutes from the dilute solution, selective
c) Cyclic
d) Loss of biological activity
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Explanation: Cyclic nature and loss of biological activity of the adsorption process are the disadvantages. The advantage of adsorption process are the ability to recover solutes from the dilute solution as well as high selective nature of the process of separation.
Sanfoundry Global Education & Learning Series – Bioseparation Science.
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