Biochemistry Questions and Answers – Protein Sequences and Evolution

This set of Biochemistry Multiple Choice Questions & Answers (MCQs) focuses on “Protein Sequences and Evolution”.

1. Amino acids sequence in DNA can be determined by the order of their _________
a) rRNA
b) tRNA
c) Nucleotides
d) mRNA
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2. Which of the following is a Sanger’s reagent?
a) 1-fluoro-2, 4-dinitrobenzene
b) 1-fluoro-2, 3-dinitrobenzene
c) 1-fluoro-2, 4-trinitrobenzene
d) 1-fluoro-2, 3-trinitrobenzene
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3. The amino acid sequences of thousands of different proteins from many species have been determined using principles first developed by?
a) Edman
b) Sanger
c) Mendel
d) Watson and Crick
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4. Which of the following compound is not involved in Edman degradation?
a) Phenylisothiocyanate
b) CF₃ COOH
c) FDNB
d) Phenylthiocarbonyl
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5. Which of the following statements is false?
a) Oxidation of cysteine residue with performic acid is done to break disulfide bond in proteins
b) Reduction of cysteine residue with dithiothreitol is done to break disulfide bond in proteins
c) Reduction of cysteine residue with performic acid is done to break disulfide bond in proteins
d) Reduced cysteine is further acetylated by iodoacetate
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6. Cleaving of peptide chain is done by _________
a) Trypsin
b) Tyrosine
c) Tryptophan
d) Arginine
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7. Which of the following is the correct order of sequencing?
a) Cleaving, sequencing and ordering
b) Sequencing, ordering and cleaving
c) Ordering, cleaving and sequencing
d) Ordering, sequencing and cleaving
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8. Edman degradation is used for _________
a) Identifying N-terminal amino acids
b) Identifying C-terminal amino acids
c) Identifying amino acid
d) Identifying carbohydrates
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9. What best summarizes the MALDI method by which gas phase ions are produced for mass spectrometry?
a) Sample is hit by a low energy xenon beam
b) Sample is forced through a narrow capillary tube and the solvent rapidly evaporates
c) Sample is embedded in a crystalline matrix and bombarded by laser beams
d) Sample is heated and then bombarded by electrons
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10. Which of the following is Edman reagent?
a) Phenylisothiocyanate
b) CF₃ COOH
c) FDNB
d) Phenylthiocarbonyl
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Sanfoundry Global Education & Learning Series – Biochemistry.

To practice all areas of Biochemistry, here is complete set of 1000+ Multiple Choice Questions and Answers.

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