Biochemistry Questions and Answers – Working with Lipids

This set of Biochemistry Multiple Choice Questions & Answers (MCQs) focuses on “Working with Lipids”.

1. Which of the following is false about lipids?
a) They are either strongly hydrophobic or amphipathic
b) They are more soluble in water
c) Extraction of lipids from tissues require organic solvents
d) They are insoluble in water
View Answer

2. Which would move faster in thin layer chromatography?
a) Beeswax
b) Phosphatidylinositol
c) Cholesterol
d) Steroid
View Answer

3. In which type of chromatography, solvents of increasing polarity are passed through a column of silica gel?
a) High performance liquid chromatography
b) Thin layer chromatography
c) Adsorption chromatography
d) Gas-liquid chromatography
View Answer

4. Phosphatidylinositol, phosphatidylglycerol and phosphatidylserine are easily separated by __________
a) Absorption chromatography
b) TLC
c) HPLC
d) Gas-liquid chromatography
View Answer

5. For the determination of fatty acid composition, transesterification is done in a warm aqueous solution of __________
a) KCl+methanol
b) KOH+methanol
c) NaOH+methanol
d) H₂O+methanol
View Answer

6. A mixture of fatty acyl methyl esters are separated based on __________
a) Charge
b) Chain length and degree of saturation
c) Molecular weight
d) Ionic size
View Answer

7. Which technique is preferred in the separation of fatty acyl methyl esters from a mixture?
a) Gas-liquid chromatography
b) Absorption chromatography
c) TLC
d) Centrifugation
View Answer

8. The dye used in TLC for detecting separated lipids by spraying the plate is __________
a) Mordant
b) Alizarin
c) Rhodamine
d) Fuchsin
View Answer

9. Which of the following is not a phospholipase?
a) A
b) C
c) D
d) K
View Answer

10. In which type of chromatography lipids are carried up a silica gel coated pate by a rising solvent front, less polar travels farther than the more polar ones?
a) Absorption chromatography
b) Thin layer chromatography
c) Gas-liquid chromatography
d) HPLC
View Answer

Sanfoundry Global Education & Learning Series – Biochemistry.

To practice all areas of Biochemistry, here is complete set of 1000+ Multiple Choice Questions and Answers.