Enzyme Technology Questions and Answers

Enzyme Technology Interview Questions and Answers

Our 1000+ Enzyme Technology questions and answers focuses on all areas of Enzyme Technology subject covering 100+ topics in Enzyme Technology. These topics are chosen from a collection of most authoritative and best reference books on Enzyme Technology. One should spend 1 hour daily for 2-3 months to learn and assimilate Enzyme Technology comprehensively. This way of systematic learning will prepare anyone easily towards Enzyme Technology interviews, online tests, examinations and certifications.


– 1000+ Multiple Choice Questions & Answers in Enzyme Technology with explanations
– Every MCQ set focuses on a specific topic in Enzyme Technology Subject

Who should Practice these Enzyme Technology Questions?
– Anyone wishing to sharpen their knowledge of Enzyme Technology Subject
– Anyone preparing for aptitude test in Enzyme Technology
– Anyone preparing for interviews (campus/off-campus interviews, walk-in interview and company interviews)
– Anyone preparing for entrance examinations and other competitive examinations
– All – Experienced, Freshers and Students


Here’s list of Questions & Answers on Enzyme Technology Subject covering 100+ topics:

1. Questions & Answers on Enzyme Kinetics Fundamentals

The section contains questions and answers on enzyme basics, enzyme nomenclature, enzyme inhibition, enzyme catalysis ph effect, temperature and ionic strength.

Introduction to Enzymes
Enzyme Nomenclature – 1
Enzyme Nomenclature – 2
Effect of pH, Temperature, Pressure, and Ionic Strength on Enzyme Catalysis
Enzyme Inhibition – 1
Enzyme Inhibition – 2

2. Questions on Enzyme Extraction and Isolation

The section contains questions on enzyme sources, enzymes isolation, enzymes extraction using chemical and physical methods.

Choice of Enzyme Sources
Extraction of Enzymes by Chemical Methods – 1
Extraction of Enzymes by Chemical Methods -2
Extraction of Enzymes by Physical Methods
Isolation of Enzymes

3. Questions & Answers on Enzymes Purification

The section contains questions and answers on organic solvent and ammonium sulphate fractionation, dialysis, ultrafiltration, gel filtration, thin layer chromatography, ion exchange chromatography, affinity chromatography, high performance and fast protein liquid chromatography.

Organic Solvent Fractionation
Ammonium Sulphate Fractionation
Thin Layer Chromatography
Gel Filtration
Ion Exchange Chromatography
Affinity Chromatography
High Performance Liquid Chromatography
Fast Protein Liquid Chromatography

4. Questions on Enzyme Kinetics

The section contains questions on enzyme action mechanism and enzyme catalysed reaction kinetics.

Mechanism of Enzyme Action – 1
Mechanism of Enzyme Action – 2
Kinetics of Enzyme Catalysed Reaction – 1
Kinetics of Enzyme Catalysed Reaction – 2

5. Questions & Answers on Immobilized Enzymes Preparation and Kinetics

The section contains questions and answers on immobilization methods and immobilized enzymes kinetics.

Methods of Immobilization – 1
Methods of Immobilization – 2
Kinetics of Immobilized Enzymes – 1
Kinetics of Immobilized Enzymes – 2
Kinetics of Immobilized Enzymes – 3

6. Questions on Large Scale Use of Enzyme in Solution

The section contains questions on enzymes application in detergent industry, protease application in food, leather and wool industries, glucose and maltose syrups production, lactases application in starch industry, enzymes medical application, enzymes in fruit juice, wine, brewing and distilling industry.

Application of Enzymes in Detergent Industry
Application of Protease in Food, Leather and Wool Industries
Use of Enzymes in Starch Industry
Production of Glucose and Maltose Syrups
Enzymes in Sucrose Industry and Glucose from Cellulose
Application of Lactases in Dairy Industry
Enzymes in Fruit Juice, Wine, Brewing and Distilling Industry
Glucose Oxidase and Catalase in Food Industry
Medical Application of Enzymes

7. Questions & Answers on Immobilized Enzymes and their Uses

The section contains questions and answers on enzyme reactors, immobilized enzyme processes, high fructose corn syrups, acrylamide preparation, amino acids and antibiotics production.

Enzyme Reactors – 1
Enzyme Reactors – 2
Enzyme Reactors – 3
Immobilized Enzyme Processes
High Fructose Corn Syrups and use of Immobilized Raffinase, Invertase and Lactase
Production of Amino Acids
Production of Antibiotics
Preparation of Acrylamide

8. Questions on Biosensors

The section contains questions on biosensors like calorimetric biosensors, potentiometric biosensors, amperometric biosensors, optical and piezoelectric biosensors, immunosensors and biosensors applications.

Calorimetric Biosensors
Potentiometric Biosensors
Amperometric Biosensors
Optical Biosensors and Piezoelectric Biosensors
Application of Biosensors – 1
Application of Biosensors – 2

9. Questions & Answers on Recent Advances in Enzyme Technology

The section contains questions and answers on enzymes reaction and stabilization in biphasic liquid and aqueous-organic systems, aqueous 2 phase systems uses, in reverse enzymes practical examples, glucosidases used in synthetic reaction and lipids interesterification.

Enzymic Reactions in Biphasic Liquid Systems
The Stabilization of Enzymes in Biphasic Aqueous-Organic Systems
Equilibria in Biphasic Aqueous-Organic Systems
Enzyme Kinetics in Biphasic Aqueous-Organic Systems
Use of Aqueous 2 Phase Systems
Practical Examples of the use of Enzymes ‘in reverse’
Glucosidases Used in Synthetic Reaction
Interesterification of Lipids

10. Questions on Enzyme Technology Future Prospects

The section contains questions on unnatural substrates, enzyme engineering, artificial enzymes and coenzyme regenerating systems.

Unnatural Substrates
Enzyme Engineering
Artificial Enzymes
Coenzyme Regenerating Systems

11. Questions & Answers on Enzyme Preparation and Usage

The section contains questions and answers on enzymes sources, enzymes preparation from clarified solution, enzymes safety and regulatory aspects.

Sources of Enzymes
Preparation of Enzymes from Clarified Solution
Preparation of Enzyme for Sale
Safety and Regulatory Aspects of Enzyme Use

12. Questions on Enzymes

The section contains questions on proenzyme and cofactors, enzyme action specificity, allosteric enzyme, isoenzyme, enzymes diagnostic uses, enzymes therapeutic and analytical uses.

Proenzyme & Cofactors – 1
Proenzyme & Cofactors – 2
Specificity of Enzyme Action
Allosteric Enzyme
Diagnostic Use of Enzymes
Therapeutic and Analytical Uses of Enzymes

13. Questions & Answers on Enzyme Extraction and Purification

The section contains questions and answers on enzymes molecular weight determination.

Determination of Molecular Weight of Enzymes

14. Questions on Enzyme Assay & Enzyme Catalysis

The sections covers questions on assays factors, continuous, discontinuous and coupled assay, enzyme assay techniques, and enzyme catalysis mechanism.

Factors Controlling in Assays
Continuous, Discontinuous and Coupled Assay
Enzyme Assay Techniques
Mechanism of Enzyme Catalysis

15. Questions & Answers on Clinical Enzymology

The section contains questions and answers on clinical significance of amylase, transaminase, proteinases, trypsinogen, creatine kinase, nucleotidase, acetylcholine esterase and glucose-6-phosphate dehydrogenase.

Clinical Significance of Amylase, Transaminase and Proteinases
Clinical Significance of Trypsinogen and Creatine Kinase
Clinical Significance of 5′ – Nucleotidase and Acetylcholine Esterase
Clinical Significance of Angiotensin Converting Enzyme and Glucose-6-phosphate Dehydrogenase
If you would like to learn Enzyme Technology thoroughly, you should attempt to work on the complete set of questions and answers mentioned above. It will immensely help anyone trying to crack an exam or an interview.

Here’s the list of Best Reference Books in Enzyme Technology.

Wish you the best in your endeavor to learn and master Enzyme Technology!

Manish Bhojasia, a technology veteran with 20+ years @ Cisco & Wipro, is Founder and CTO at Sanfoundry. He is Linux Kernel Developer & SAN Architect and is passionate about competency developments in these areas. He lives in Bangalore and delivers focused training sessions to IT professionals in Linux Kernel, Linux Debugging, Linux Device Drivers, Linux Networking, Linux Storage, Advanced C Programming, SAN Storage Technologies, SCSI Internals & Storage Protocols such as iSCSI & Fiber Channel. Stay connected with him @ LinkedIn