This set of Biochemistry Multiple Choice Questions & Answers (MCQs) focuses on “RNA Processing”.
1. Which of the following statements regarding splicing in eukaryotes is correct?
a) Several reactions in the splicing process involve hydrolysis of ATP
b) Exons are spliced out and introns are retained in the mature mRNA transcript
c) Splicing takes place in the cytosol
d) Small nuclear RNAs are retained in the mature mRNA transcript
View Answer
Explanation: Splicing takes place within the nucleus.
Introns are spliced out and exons are retained in the mature mRNA transcript.
2. Which of the following is not involved in the post transcriptional processing of t-RNA?
a) Base modulation
b) Attachment of CCA arm
c) Splicing
d) Attachment of poly-A tail
View Answer
Explanation: There is no poly A tail in mature tRNA. Poly A tail is the common change occurring in the eukaryotic mRNA.
3. Which of the following is an incorrect statement about m-RNA?
a) Cap is added to the 5’ end
b) Introns are removed and exons are spliced together
c) Histone mRNAs lack 5’ cap
d) Poly-A tail is added to the 3’ end
View Answer
Explanation: Introns are removed and exons are ligated.
4. Which is the first nucleic acid synthesizing enzyme discovered?
a) Polynucleotide phosphorylase
b) DNA polymerase
c) RNA polymerase
d) DNA ligase
View Answer
Explanation: Polynucleotide phosphorylase is the first nucleic acid synthesizing enzyme discovered.
5. 70S prokaryotic ribosome is the complex of ____________
a) 30S + 50S
b) 30S + 40S
c) 20S + 60S
d) 20S + 30S
View Answer
Explanation: 70S prokaryotic ribosome is the complex of 30S and 50S subunits.
6. 80S eukaryotic ribosome is the complex of ____________
a) 60S and 40S
b) 40S and 20S
c) 60S and 50S
d) 30S and 20S
View Answer
Explanation: 80S eukaryotic ribosome is the complex of 60S and 40S subunits.
7. What is the main function of t-RNA?
a) Proof reading
b) Inhibits protein synthesis
c) Identifies amino acids and transport them to ribosomes
d) None of the mentioned
View Answer
Explanation: t-RNA identifies amino acids and transports them to ribosomes.
8. Which one of the following best describes the cap modification of eukaryotic mRNA?
a) Modified guanine nucleotide added to the 3’ end of the transcript
b) Modified guanine nucleotide added to the 5’ end of the transcript
c) String of adenine nucleotides added to the 3’ end of the transcript
d) String of adenine nucleotides added to the 5’ end of the transcript
View Answer
Explanation: Modified guanine nucleotide added to the 5’ end of the transcript, this best describes the cap modification of eukaryotic mRNA.
9. The largest class of introns which are found in nuclear mRNA primary transcript is?
a) Spliceosomal introns
b) Group I introns
c) Group II introns
d) Group IV introns
View Answer
Explanation: Spliceosomal introns are the largest class of introns which are found in nuclear mRNA primary transcript.
10. Which type of splicing reaction requires a guanine nucleoside or nucleotide cofactor that is not used as a source of energy?
a) Spliceosomal
b) Group I
c) Group II
d) Group IV
View Answer
Explanation: Group I type of splicing reaction requires a guanine nucleoside or nucleotide cofactor that is not used as a source of energy.
Sanfoundry Global Education & Learning Series – Biochemistry.
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