Enzyme Technology Questions and Answers – Recent Advances – Interesterification of Lipids

This set of Enzyme Technology Multiple Choice Questions & Answers (MCQs) focuses on “Recent Advances – Interesterification of Lipids”.

1. The reactions which involve carboxyl group exchange between esters with the help of transferases is referred to as ___________
a) acidolyses
b) alcoholyses
c) isomerization
d) transesterifications
View Answer

Answer: d
Explanation: The reactions which involve carboxyl group exchange between esters with the help of transferases is referred to as transesterifications. The reaction which involves carboxyl group exchange between esters and carboxylic acids is referred to as acidolyses. Alcoholyses is reaction that involves transferases and allows exchange of alcohol between esters and alcohol. Isomerization involves forming isomers of a compound using isomerases.
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2. _______________ is a reaction which involve carboxyl group exchange between esters and carboxylic acids by use of transferases.
a) Alcoholyses
b) Transesterifications
c) Acidolyses
d) Isomerization
View Answer

Answer: c
Explanation: Acidolyses is a reaction which involves carboxyl group exchange between esters and carboxylic acids by use of transferases. Transesterification and alcoholyses involves transferases enzyme which allows carboxyl group exchange between esters and exchange of alcohol between esters and alcohols respectively. Isomerization involves forming isomers of a compound using isomerases.

3. Alcoholyses allows the exchange of alcohol between esters and alcohols by use of _________ enzyme.
a) dextransucrase
b) fructosyltransferases
c) transferase
d) rennet
View Answer

Answer: c
Explanation: Alcoholyses allows the exchange of alcohol between esters and alcohols by use of transferase enzyme. Dextransucrase can be used to produce dextran from sucrose. Fructosyltransferases synthesize compounds such as sucrose-6-acetate, xylosucrose and neosugar. Rennet is a protease enzyme and which is used cheese making process.

4. Cocoa butter may be produced by using ________ acting as transacylases.
a) lipases
b) transferase
c) papain
d) dextransucrase
View Answer

Answer: a
Explanation: Cocoa butter may be produced by using lipases acting as transacylases. Transferases is an enzyme which is used different types of reaction such as transesterifications, acidolyses and alcoholyses. Papain is a protease enzyme obtained from papaya and is uses in meat tenderization. Dextransucrase can be used to produce dextran from sucrose.
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5. For which of the following, acidolysis reaction is not required?
a) Increasing the value of rapeseed oil
b) Cheese making
c) Increasing the value of palm oil
d) Increasing the value of palm oil and sunflower oil
View Answer

Answer: b
Explanation: For cheese making process, rennet is used to coagulate the milk. Acidolysis reaction is required for the following:
• Increasing the value of rapeseed oil by exchanging linoleic acid for linolenic acid residues.
• Increasing the value of palm oil and sunflower oil by increasing their content of oleic acid residues.
• Increasing the value of sunflower oil by increasing their content of oleic acid residues.

6. Cocoa butter is a fat which is not used in confectionery.
a) True
b) False
View Answer

Answer: b
Explanation: The above statement is false. Cocoa butter is an expensive fat that is used in confectionary because of the sharp melting point it has between room temperature and body temperature that the chocolate almost melts in the mouth. This happens due to small variations in the structure of triglycerides with 80 % palmitic acid or stearic acid in the 1 and 3 position with oleic acid in the central 2 position.

7. What does ‘X’ represent in the following reaction?
Ethyl acetate + isoamylalchohol \(\underset{Lipase}{\rightleftharpoons}\) X + ethanol
a) Isoamyl acetate
b) Cocoa butter
c) N-acetyllactosamine
d) Dextran
View Answer

Answer: a
Explanation: In the above reaction, ‘X’ represents Isoamyl acetate, which is a natural aroma. In the above reaction, lipase is being used in an alcoholysis reaction to produce isoamyl acetate by the use of a biphasic system. Cocoa butter may be produced by using lipases ac. ting as transacylases. N-acetyllactosamine is synthesized by using β-galactosidase from N-acetylglucosamine. Dextran is produced from sucrose by using dextransucrase.
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Manish Bhojasia, a technology veteran with 20+ years @ Cisco & Wipro, is Founder and CTO at Sanfoundry. He is Linux Kernel Developer & SAN Architect and is passionate about competency developments in these areas. He lives in Bangalore and delivers focused training sessions to IT professionals in Linux Kernel, Linux Debugging, Linux Device Drivers, Linux Networking, Linux Storage, Advanced C Programming, SAN Storage Technologies, SCSI Internals & Storage Protocols such as iSCSI & Fiber Channel. Stay connected with him @ LinkedIn | Youtube | Instagram | Facebook | Twitter