This set of Enzyme Technology Multiple Choice Questions & Answers (MCQs) focuses on “Enzymes Purification – Fast Protein Liquid Chromatography”.
1. The chromatography technique in which the stationary phase may be solid or liquid is referred to as ________________
a) ion-exchange chromatography
b) thin layer chromatography (TLC)
c) high performance liquid chromatography (HPLC)
d) fast protein liquid chromatography (FPLC)
Explanation: In FPLC, the stationary phase is a solid or liquid in the form of matrix. In TLC, the stationary phase is immobilized on a glass plate and organic solvent acts as mobile phase. In HPLC, the sample is forced into the column at high pressure. Ion-exchange chromatography refers to use of ions for separation of the molecules.
2. In which chromatographic technique, the solvent velocity and constant flow rate is controlled?
a) Affinity chromatography
b) Ion-exchange chromatography
c) Fast protein liquid chromatography (FPLC)
d) Gel permeation
Explanation: During FPLC, the solvent velocity and constant flow rate is controlled by using pumps. Affinity chromatography is a technique which separate molecules based on the affinities between different molecules. Gel permeation allows separation based on the size of the separating molecule. Ion-exchange chromatography is a technique which utilizes ion for separation of molecules.
3. In FPLC, the sample separates into various zones of sample components and is referred to as ________
a) retention time
Explanation: pKa is the pH at which half the groups are ionized. Retention time is the time at specific analytes elute out. Peaks are formed as result in chromatographs. Peaks and retention time is seen during HPLC. In FPLC, the sample is introduced and carried into the column by the mobile phase. As the solvent is forced by the flow rate, the sample separates into various zones of sample components and is referred to band.
4. Which characteristic of the enzyme can be used for purifying it by using FPLC?
a) Molecular weight
c) Electric charge
Explanation: Enzyme substrate interaction is utilized while performing affinity chromatography. Enzyme possessing various characteristic such as molecular weight, electric charge and hydrophobicity can be purified by adopting FPLC.
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