Enzyme Technology Questions and Answers – Recent Advances – Glucosidases Used in Synthetic Reaction

This set of Enzyme Technology Problems focuses on “Recent Advances – Glucosidases Used in Synthetic Reaction”.

1. Use of glycosidases in synthetic reactions is a comparatively neglected topic.
a) True
b) False
View Answer

Answer: a
Explanation: Use of glycosidases in synthetic reactions is comparatively neglected topic because of the following reasons:
• Polysaccharides can be obtained readily from plant or microbial sources.
• Polysaccharide function is not so specifically related to its structure.
• The theory describing the functional significance of oligosaccharides is still being developed.
• As there is an abundance of knowledge about glycosidases and their specificities, there is no fundamental reason why they should not be used to synthesize oligosaccharides.
Hence the above statement is true.
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2. Which of the following enzyme is not used to construct oligosaccharides?
a) Dextransucrase
b) Levansucrase
c) Papain
d) Cyclomaltodextrin glucanotransferase
View Answer

Answer: c
Explanation: The enzymes used to construct oligosaccharides such as glucose, fructose or galactose residues are dextransucrase, levansucrase, cyclomaltodextrin glucanotransferase and β-galactosidase. Papain is a protease obtained from papaya and is used in meat tenderization.

3. Which of the following compounds are synthesized by fructosyltransferases?
a) Sucrose-6-acetate
b) Xylosucrose
c) L-aspartate
d) Neosugar
View Answer

Answer: c
Explanation: Fructosyltransferases are used to synthesize compounds such as sucrose-6-acetate, xylosucrose and the low cariogenic sweetener, ‘neosugar’. L-aspartate is produced from L-aspargine using asparginase enzyme.

4. ____________ is a low cariogenic sweetener, which consists of a mixture of glucose, sucrose and β-2,1 linked fructans with terminal non-reducing glucose residues.
a) Neosugar
b) Xylosucrose
c) Lactose
d) Sucrose
View Answer

Answer: a
Explanation: Neosugar is a low cariogenic sweetener, which consists of a mixture of glucose, sucrose and β-2,1 linked fructans with terminal non-reducing glucose residues. Xylosucrose is β-D-fructofuranose-(2,1)-a-D-xylopyranoside which is synthesized by fructosyltransferases. Lactose is a disaccharide made up of glucose and galactose. Sucrose is also a disaccharide made up of glucose and fructose.

5. _____________is used to produce dextran from sucrose.
a) Fructosyltransferases
b) Dextransucrase
c) Papain
d) Streptokinase
View Answer

Answer: b
Explanation: Dextran which is a polymer of a-1,6 linked glucose residues, is produced from sucrose using dextransucrase. Fructosyltransferases are used to synthesize compounds such as sucrose-6-acetate, xylosucrose and neosugar’. Papain is a protease obtained from papaya and is used in meat tenderization. Streptokinase is used in the treatment of blood clots and it converts plasminogen to plasmin.

6. ___________ synthesizes N-acetyllactosamine using a carbon-celite column to remove it, as soon as it is formed.
a) Dextransucrase
b) Streptokinase
c) β – galactosidase
d) Ribonuclease
View Answer

Answer: c
Explanation: N-acetyllactosamine is a β-D-galactopyranosyl-(1,6)-2-acetamido-2-deoxy-D-glucose which is synthesized by using β – galactosidase using a carbon-celite column to remove it, as soon as it is formed. Dextransucrase is used to produce dextran from sucrose. Streptokinase is used in the treatment of blood clots and it converts plasminogen to plasmin. Ribonuclease has an antiviral activity that acts by hydrolyzing RNA.

Sanfoundry Global Education & Learning Series – Enzyme Technology.

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Manish Bhojasia, a technology veteran with 20+ years @ Cisco & Wipro, is Founder and CTO at Sanfoundry. He is Linux Kernel Developer & SAN Architect and is passionate about competency developments in these areas. He lives in Bangalore and delivers focused training sessions to IT professionals in Linux Kernel, Linux Debugging, Linux Device Drivers, Linux Networking, Linux Storage, Advanced C Programming, SAN Storage Technologies, SCSI Internals & Storage Protocols such as iSCSI & Fiber Channel. Stay connected with him @ LinkedIn | Youtube | Instagram | Facebook | Twitter