Biochemistry Questions and Answers – DNA Cloning: The Basics-1

This set of Biochemistry Multiple Choice Questions & Answers (MCQs) focuses on “DNA Cloning: The Basics-1”.

1. Which of the following does not act as a restriction enzyme?
a) EcorI
b) BamHI
c) HindIII
d) polydeoxyribonucleotide synthase
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2. Which of the following enzyme is responsible for making a DNA copy from RNA?
a) Reverse transcriptase
b) DNA polymerase
c) RNA polI
d) RNA polII
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3. Which of the following statements is true?
a) Exonuclease III removes nucleotide residues from the 3’ ends of a DNA strand
b) Bacteriophage lambda exonuclease removes terminal phosphates
c) Alkaline phosphatase removes nucleotides from 5‘ends
d) Kinase adds homopolymer tails to the 3’ –OH ends of a linear duplex
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4. Which of the following can be termed as a restriction modification system?
a) Restriction endonuclease + methylase
b) DNA ligase + methylase
c) Restriction endonuclease + acetylase
d) DNA ligase + acetylase
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5. Which of the following is true about restriction endonucleases?
a) Type I and II requires ATP to move along DNA
b) Type I, II and III requires ATP to move along DNA
c) Type II requires no ATP and cleaves DNA within recognition sequence
d) Type II requires ATP and cleaves DNA within recognition sequence
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6. Which of the following enzymes’ combined action leads to the generation of sticky ends in the plasmid vector?
a) Alkaline phosphatase and terminal transferase
b) Exonuclease III and alkaline phosphatase
c) Bacteriophage lambda exonuclease and terminal transferase
d) Exonuclease III and terminal transferase
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7. Generally a plasmid vector contains how many elements?
a) 1
b) 2
c) 3
d) 4
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8. To make the recombinant plasmid permeable to DNA molecules, which of the chemicals is added?
a) MgCl₂
b) CaCl₂
c) NaCl
d) HCl
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9. Which of the following statements is false?
a) The cells which take up recombinant plasmids are drug resistant
b) The cells with no recombinant plasmids are drug resistant
c) Plasmid replication is independent of cell’s division
d) The cells containing recombinant plasmid are called transformed cells
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10. The extra chromosomal, self-replicating, closed, double stranded and circular DNA molecule is generally termed as __________
a) Chromosome
b) Plasmid
c) Genomic DNA
d) Bacteriophage
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11. E.cor1 is a __________
a) DNA ligase enzyme
b) Restriction endonuclease
c) A vector used for insulin synthesis
d) A plasmid used as a vector
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12. In which stage of genetic engineering a probe is used?
a) Cleaving DNA
b) Recombining DNA
c) Cloning
d) Screening
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13. What is a DNA library?
a) A DNA fragment inserted into a vector
b) A general collection of all genes sequenced thus far
c) All DNA fragments identified with a probe
d) A collection of DNA fragments that make up the entire genome of a particular organism
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14. Which of the following enzyme is required for end to end joining of DNA?
a) DNA ligase
b) Restriction endonuclease
c) RNA polymerase
d) DNA polymerase
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15. Repressor molecules bind to __________
a) Operator
b) Promoter
c) Enhancer
d) Hormone response element
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Sanfoundry Global Education & Learning Series – Biochemistry.

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