This set of Biophysics Multiple Choice Questions & Answers (MCQs) focuses on “Adsorption Chromatography”.
1. What type of adsorption chromatography uses gas as a mobile phase?
a) Gas-Solid Chromatography
b) Liquid-Solid Chromatography
c) Gas-Liquid Chromatography
d) Gas – Gas Chromatography
View Answer
Explanation: Gas-Solid chromatography uses gas as a mobile phase. The adsorption phenomenon must involve the adsorption of molecules over a solid surface.
2. What type of adsorption chromatography uses the liquid as a mobile phase?
a) Gas-Solid Chromatography
b) Liquid-Solid Chromatography
c) Gas-Liquid Chromatography
d) Gas – Gas Chromatography
View Answer
Explanation: Liquid-Solid Chromatography uses the liquid as a mobile phase. The adsorption of molecules happens on a solid surface. In a Liquid-Solid Chromatography, liquid works as a mobile phase.
3. For the separation of DNA, what material is used as an absorbent?
a) Alumina
b) Potassium phosphate
c) Calcium phosphate
d) Magnesium phosphate
View Answer
Explanation: Calcium phosphate is used as an absorbent for the separation of DNA. This is because Calcium phosphate perfectly binds to the double-stranded DNA.
4. Calcium phosphate is mostly used for the separation of RNA.
a) True
b) False
View Answer
Explanation: Calcium phosphate is used only to separate DNA molecules as it perfectly binds to double-stranded Nucleic acid. RNA molecules are separated through gel electrophoresis.
5. _________________ can be used for the separation of DNA or double-stranded RNA.
a) Aluminum Hydroxide
b) Hydroxyapatite or CaSo4
c) Potassium phosphate
d) Magnesium phosphate
View Answer
Explanation: Hydroxyapatite or CaSo4 is used for the separation of DNA or double-stranded RNA. Hydroxyapatite only binds to the double-stranded Nucleic acid.
6. Which material cannot be used as a stationary phase in adsorption chromatography?
a) Silicic acid (silica gel)
b) Aluminum oxide
c) Calcium carbonate
d) Hexane
View Answer
Explanation: To be a good adsorbent the material must be solid, but hexane is a liquid compound. Therefore, Hexane cannot be used as a stationary phase in adsorption chromatography.
7. What is used for the elution of the sample?
a) Mobile phase constituents
b) Stationary phase constituents
c) Both Mobile and stationary phase constituents
d) Some constitutions of stationary phase constituents
View Answer
Explanation: Elution is the actual separation process. The difference in absorptivity in the mobile phase over the stationary is the principle of separation. So, the mobile phase is loaded after sample loading in chromatography which facilitates the elution process.
8. Which are the oldest chromatographic techniques?
a) High-pressure liquid chromatography
b) Thin layer chromatography
c) Gas-liquid chromatography
d) Adsorption chromatography
View Answer
Explanation: Adsorption chromatography is the oldest chromatographic techniques. Initially, leaf samples (and others) were separated by adsorption chromatography.
9. How silica gel is activated for adsorption chromatography?
a) By applying low pressure
b) By heating the chromatographic plate at 400 – 500°C
c) By heating the chromatographic plate at 150 – 250°C
d) By applying high pressure
View Answer
Explanation: Silica gel is activated by heating the chromatographic plate at 150 – 250°C. If a water molecule is present in the gel, it will interfere with the movement of the mobile phase over the stationary phase.
10. Imagine you forgot to activate silica gel (by heating) before your chromatography experiment. What type of chromatography experiment you have actually performed?
a) Partition chromatography
b) Adsorption chromatography
c) Absorption chromatography
d) Column chromatography
View Answer
Explanation: Water evaporation due to heating, makes the plate suitable for adsorption. If water is present in the plate the chromatography experiment will be a partition chromatography, where water will act as a liquid stationary phase.
11. What temperature is used to active alumina stationary phage for adsorption chromatography?
a) 100°C
b) 400°C
c) 200°C
d) 500°C
View Answer
Explanation: 400°C temperature is used to active alumina stationary phage for adsorption chromatography. Alumina contains water in its complex. To evaporate the complex water molecule high temperature is required.
12. Which stationary phase is the best for the separation of aromatics from the olefins?
a) Silicic acid (silica gel)
b) Calcium carbonate
c) Al2O3
d) Calcium phosphate
View Answer
Explanation: Aromatic compounds are polar molecules. Therefore, the strong positive field of Al+++ can easily separate aromatics compounds from nonpolar olefins.
13. What is the pore size of mesoporous activated charcoal?
a) 50 mm
b) 25 μm
c) 50 μm
d) 2 – 50 nm
View Answer
Explanation: The pore size of mesoporous activated charcoal is 2 – 50 nm. The pore size of microspores activated charcoal is < 2 nm. The less pore size, the charcoal is more effective.
14. TLC and paper chromatography is a type of _____________ chromatography.
a) absorption
b) adsorption
c) column
d) gas
View Answer
Explanation: TLC and paper chromatography is a type of planner adsorption chromatography. In both cases, the samples are separated on the basis of their adsorption to the stationary phase.
15. In which of the following options, dipole-dipole attraction is found?
a) Nonpolar stationery and nonpolar mobile phase
b) Polar stationery and nonpolar mobile phase
c) Polar stationery and polar mobile phase
d) Nonpolar stationery and polar mobile phase
View Answer
Explanation: Dipole-dipole attraction happens between two dipoles. The polar molecules can form a dipole. Therefore, in polar stationery and polar mobile phase, dipole-dipole attraction can be found.
Sanfoundry Global Education & Learning Series – Biophysics.
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