This set of Cell Biology Multiple Choice Questions & Answers (MCQs) focuses on “Techniques – Cell Culture”.
1. Trypsin is used for dissociating the tissue into single cells.
a) True
b) False
View Answer
Explanation: Trypsin is a proteolytic enzyme and is used for dissociating the tissue into single cells. During the primary culture, tissue samples are taken generally from the embryos rather than the adult organism because of easy dissociation.
2. In the secondary culture, cells are obtained from _______________________
a) primary culture
b) the organism
c) organ culture
d) phenotypic culture
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Explanation: The culture that is done after the primary culture is known as the secondary culture (the process termed passaging or subculturing). The primary culture is obtained from the organism itself.
3. EDTA binds the ______________ ions.
a) magnesium
b) iron
c) carbon
d) calcium
View Answer
Explanation: EDTA (ethylenediamine tetracetate) is a chelating agent that binds to calcium ions and prevents cellular adhesion. A chelating agent reacts with metal ions to form a water-soluble complex.
4. HeLa cells are a cell line.
a) True
b) False
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Explanation: If a homogenous cell culture has the ability to grow indefinitely in vitro, it is termed a cell line. Cell lines would never undergo apoptosis and senescence. HeLa is a cancerous cell line derived from humans and was the first line to be maintained.
5. Under favorable conditions, the protoplasts can grow into a ____________________
a) callus culture
b) organ culture
c) root culture
d) shoot culture
View Answer
Explanation: Under favorable conditions and chemically defined media, the protoplasts can grow into an undifferentiated cell mass, known as the callus culture. Protoplasts are the cells whose plasma membranes have been removed.
6. The process of dedifferentiation in cell culture can give rise to ________________________
a) induced-pluripotent stem cells
b) carcinoma cells
c) single protoplasts
d) fused protoplasts
View Answer
Explanation: Dedifferentiation is the process by which the differentiated cells reprogram and go back to their precursor counterparts. The cells that are induced to undergo dedifferentiation are termed as induced pluripotent stem cells.
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