This set of Cell Biology Question Paper focuses on “Techniques – Differential Centrifugation”.
1. Isolation of an organelle in bulk quantity can be accomplished by _________________________
c) differential centrifugation
d) mass spectroscopy
Explanation: For studying a particular organelle, it is first important to isolate it in a bulk quantity. Differential centrifugation is the technique of separation of different organelles of the cell based on their relative densities.
2. The isotonic buffer solution prevents _____________________
a) change in pressure
b) change in temperature
c) cell lysis
d) membrane rupture
Explanation: In the steps towards performing differential centrifugation, the cells are lysed by mechanical homogenization and then immersed in hypotonic solution (containing sucrose) that prevents rupture of the organelle membranes.
3. Density gradient centrifugation uses __________________
Explanation: The density gradient centrifugation uses a sucrose gradient. In this technique, the compounds are separated based on their densities by forming separate layers after the centrifugation run.
4. Which of the following can be used to study cellular activities?
b) cell-free systems
d) liquid chromatography
Explanation: The cell-free systems can be used to study cellular activities. Organelles isolated using differential centrifugation and subsequent density gradient centrifugation can be used for studying their functions using the cell-free systems.
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