Cell Biology Questions and Answers – Techniques – Differential Centrifugation

This set of Cell Biology Question Paper focuses on “Techniques – Differential Centrifugation”.

1. Isolation of an organelle in bulk quantity can be accomplished by _____________
a) spectroscopy
b) spectrophotometry
c) differential centrifugation
d) mass spectroscopy
View Answer

Answer: c
Explanation: For studying a particular organelle, it is first important to isolate it in a bulk quantity. Differential centrifugation is the technique of separation of different organelles of the cell based on their relative densities.
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2. The isotonic buffer solution prevents _________
a) change in pressure
b) change in temperature
c) change in pH
d) membrane rupture
View Answer

Answer: d
Explanation: Isotonic solutions have the same solute concentration as inside the cell or organelle. This prevents the cell/organelle from osmotic pressure thus preventing lysis or rupture of membrane.

3. Density gradient centrifugation is a type of isopycnic centrifugation.
a) True
b) False
View Answer

Answer: a
Explanation: Isopycnic centrifugation solely relies on density for separation. In density gradient centrifugation too the contents are separated with the help of an additive with intermediate density of the particles that are being separated. It is therefore a form of Isopycnic separation.

4. Which of the following can be used to study cellular activities?
a) Diffraction
b) Cell-free systems
c) Autoradiography
d) Liquid chromatography
View Answer

Answer: b
Explanation: Organelles that are isolated from the cells can be suspended into a medium that mimics the in-vivo conditions of the cell. This is known as a cell free system. This can be used to study interactions between organelles.
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Manish Bhojasia, a technology veteran with 20+ years @ Cisco & Wipro, is Founder and CTO at Sanfoundry. He is Linux Kernel Developer & SAN Architect and is passionate about competency developments in these areas. He lives in Bangalore and delivers focused training sessions to IT professionals in Linux Kernel, Linux Debugging, Linux Device Drivers, Linux Networking, Linux Storage, Advanced C Programming, SAN Storage Technologies, SCSI Internals & Storage Protocols such as iSCSI & Fiber Channel. Stay connected with him @ LinkedIn | Instagram | Facebook | Twitter