Cell Biology Questions and Answers – Techniques – Differential Centrifugation

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This set of Cell Biology Question Paper focuses on “Techniques – Differential Centrifugation”.

1. Isolation of an organelle in bulk quantity can be accomplished by _________________________
a) spectroscopy
b) spectrophotometry
c) differential centrifugation
d) mass spectroscopy
View Answer

Answer: c
Explanation: For studying a particular organelle, it is first important to isolate it in a bulk quantity. Differential centrifugation is the technique of separation of different organelles of the cell based on their relative densities.
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2. The isotonic buffer solution prevents _____________________
a) change in pressure
b) change in temperature
c) cell lysis
d) membrane rupture
View Answer

Answer: d
Explanation: In the steps towards performing differential centrifugation, the cells are lysed by mechanical homogenization and then immersed in hypotonic solution (containing sucrose) that prevents rupture of the organelle membranes.

3. Density gradient centrifugation uses __________________
a) sucrose
b) pentose
c) glucose
d) dextrose
View Answer

Answer: a
Explanation: The density gradient centrifugation uses a sucrose gradient. In this technique, the compounds are separated based on their densities by forming separate layers after the centrifugation run.

4. Which of the following can be used to study cellular activities?
a) diffraction
b) cell-free systems
c) autoradiography
d) liquid chromatography
View Answer

Answer: a
Explanation: The cell-free systems can be used to study cellular activities. Organelles isolated using differential centrifugation and subsequent density gradient centrifugation can be used for studying their functions using the cell-free systems.
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Manish Bhojasia, a technology veteran with 20+ years @ Cisco & Wipro, is Founder and CTO at Sanfoundry. He is Linux Kernel Developer & SAN Architect and is passionate about competency developments in these areas. He lives in Bangalore and delivers focused training sessions to IT professionals in Linux Kernel, Linux Debugging, Linux Device Drivers, Linux Networking, Linux Storage, Advanced C Programming, SAN Storage Technologies, SCSI Internals & Storage Protocols such as iSCSI & Fiber Channel. Stay connected with him @ LinkedIn