Agricultural Biotechnology Questions and Answers – Agriculture – Cell and Tissue Culture Techniques

This set of Agricultural Biotechnology Multiple Choice Questions & Answers (MCQs) focuses on “Agriculture – Cell and Tissue Culture Techniques”.

1. Cell culture technology is growing cells outside of living organisms.
a) True
b) False
View Answer

Answer: a
Explanation: With mammalian cell culture, animal testing can be replaced by cell testing. This can be done while evaluating the safety and efficiency of medicines. Cellular therapists are one of the horizons as well.

2. New healthy cells produced by ______ could replace the malfunctioning cells in people with Parkinson’s disease.
a) Tissue culture
b) Embryonic stem cell culture
c) DNA fingerprinting
d) Genetically engineering micro-organisms
View Answer

Answer: b
Explanation: The new cells produced by this technique can also restore function in stroke and heart attack victims. Plant cell culture produces environmentally sound option for obtaining naturally occurring products.

3. Which is the technology used to obtain ‘Taxol’?
a) Protein engineering
b) Plant cell culture
c) Tissue culture
d) Somaclonal variations
View Answer

Answer: b
Explanation: Plant cell culture is an economically feasible option for obtaining products with therapeutic value, such as chemotherapeutic agent paclitaxel, a compound found in yew trees and marketed under the name Taxol.

4. Cell culture can be used to grow micro-organisms that kill insect pests.
a) True
b) False
View Answer

Answer: a
Explanation: By using cell culture micro-organisms can be grown that infect insects. Biocontrol agents infect only certain insects without harming beneficial insects, such as honeybees.

5. What is the common name for Fragaria?
a) Boston ferns
b) Strawberries
c) Carnations
d) African violets
View Answer

Answer: b
Explanation: The simplest tissue culture involves multiplication of easy and fast growing plant material. The Latin name for Boston ferns is Nephrolepis. The Latin name for African violets is Saintpaulia.

6. What is the botanical name for fig?
a) Ficus carica
b) Psidium guajava
c) Pyrus communis
d) Ananas comosus
View Answer

Answer: a
Explanation: The botanical name for guava is Psidium guajava. The botanical name for pear is Pyrus communis. The botanical name for pineapple is Ananas comosus.

7. What is the type of explant found in lemon?
a) Anther
b) Nucellus
c) Shoot tip
d) Stem tip
View Answer

Answer: b
Explanation: The botanical name for lemon is Citrus limon. It is seen that there is a direct adventive embryo formation. Shoot tip is mostly seen in fig and grapes.

8. What is the common name for Mangifera indica?
a) Papaya
b) Mango
c) Mandarin
d) Apple
View Answer

Answer: b
Explanation: Type of explant seen in mango is nucellus and embryo. It often responses to somatic embryogenesis and hybrid plantlets.

9. What is the Latin name for sapota?
a) Prunus sapota
b) Punica sapota
c) Achrus sapota
d) Fragaria sapota
View Answer

Answer: c
Explanation: The type of explant for sapota is endosperm. Callus formation is seen. Sapota is a rich fruit compared to apple.

10. In which year was the virus-free plant dahlia regenerated?
a) 1950
b) 1952
c) 1954
d) 1956
View Answer

Answer: b
Explanation: In 1952, Morel and Martin regenerated a virus free dahlia plant. This was done by the excision of some meristematic domes from virus infected shoots.

11. Which was the species from which virus A and Y were eliminated for the first time?
a) Tomato
b) Potato
c) Lime
d) Papaya
View Answer

Answer: b
Explanation: In 1955, Morel and Martin eliminated virus A and Y from a virus infected potato. Semal and Lepoivre (1992) reported that a virus-free sweet potato was producing 40T/ha in China by comparison of the 20T/ha produced before meristem culture.

12. What is the size of the excised meristem that produced plants containing mottle virus?
a) 0.2
b) 0.4
c) 0.5
d) 0.7
View Answer

Answer: d
Explanation: The size of the excised meristem should be as small as possible. The tips between 0.2-0.5 mm most frequently produces virus free carnation plants. The explants smaller than 0.2 mm cannot survive.

13. Who among the following eradicated virus diseases from Spanish Citrus orchards?
a) Morel
b) Martin
c) Navarro
d) Mosella
View Answer

Answer: c
Explanation: In 1975, Navarro et al. eradicated virus diseases from the above mentioned plant. Mosella et al. (1980) used this technique to eliminate virus diseases from peach trees.

14. What was the technique used to produce Pinus radiata in 1987?
a) Haplodiploidisation
b) Hedging system
c) Somaclonal variations
d) Germplasm conversation
View Answer

Answer: b
Explanation: This was done in 1987 by Aitken Christie and Jones. In 1990 the technique was proposed to corn plants. The internodes and leaves were reduced by the introduction of an anti-gibberellin agent in the medium.

15. What is the damage caused to US each year by the pest called the European corn borer?
a) $1 billion
b) $1.2 billion
c) $1.4 billion
d) $1.6 billion
View Answer

Answer: b
Explanation: The European corn borer is one of the most prevalent pests. Pheromones are used to attract insects away from crop plants. This helps in pest control.

Sanfoundry Global Education & Learning Series – Agricultural Biotechnology

To practice all areas of Agricultural Biotechnology, here is complete set of 1000+ Multiple Choice Questions and Answers.

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Manish Bhojasia - Founder & CTO at Sanfoundry
Manish Bhojasia, a technology veteran with 20+ years @ Cisco & Wipro, is Founder and CTO at Sanfoundry. He lives in Bangalore, and focuses on development of Linux Kernel, SAN Technologies, Advanced C, Data Structures & Alogrithms. Stay connected with him at LinkedIn.

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