This set of Genetic Engineering Multiple Choice Questions & Answers (MCQs) focuses on “YAC and Expression Systems”. 1. When YAC is used to clone DNA. What is the size of the DNA that can be cloned? a) Large (upto megabases) b) Small (upto few hundred bases) c) No size restriction d) Medium (upto kilobases) 2. … Read more
This set of Genetic Engineering Multiple Choice Questions & Answers (MCQs) focuses on “Markers”. 1. One of the commonly used markers is the URA3 gene. It encodes for ______ biosynthesis. a) adenine b) uracil c) guanine d) cytosine 2. CAN1 gene encodes a permease that causes uptake of _________ analogue. a) toxic guanidine b) toxic … Read more
This set of Genetic Engineering Multiple Choice Questions & Answers (MCQs) focuses on “Bacteria”. 1. An organism containing a gene which doesn’t belong to it and is derived from somewhere else then the organism is said to be ___________ a) transformed b) transgenic c) mutant d) modified 2. E.coli is a gram negative bacterium. a) … Read more
This set of Genetic Engineering Multiple Choice Questions & Answers (MCQs) focuses on “Gene Function and Gene Trapping”. 1. How many types of reporter gene constructs are there? a) 1 b) 2 c) 3 d) 4 2. Desired changes are made in the structure of the promoter and is ligated onto reporter gene. The alteration … Read more
This set of Genetic Engineering Questions and Answers for Aptitude test focuses on “Co-expression of Proteins and Optimization”. 1. If it is desirable to express two or more proteins simultaneously, then it is known as ___________ a) hybrid of proteins b) fusion of proteins c) co-expression of proteins d) combination of proteins 2. For co-expression … Read more
This set of Genetic Engineering Multiple Choice Questions & Answers (MCQs) focuses on “Fusion Proteins”. 1. There are some advantages of expressing protein as a fusion protein. It may enhance stability, folding ______ and ______ formation. a) solubility, phosphodiester bond formation b) insolubility, phosphodiester bond formation c) solubility, disulphide bond formation d) insolubility, disulphidebond formation … Read more
This set of Genetic Engineering Multiple Choice Questions & Answers (MCQs) focuses on “Promoters”. 1. Some vectors carry a mutant form of promoter known as lacUV5 promoter. It carries ______ in the promoter region and _____ the efficiency. a) point mutations, decreases b) point mutations, increases c) frameshift mutations, increases d) frameshift mutations, increases 2. … Read more
This set of Genetic Engineering Multiple Choice Questions & Answers (MCQs) focuses on “Expression in Vivo”. 1. If overexpression of proteins takes place, they become toxic at times. In such a case _______ colonies are recovered after ______ with the recombinant plasmid. a) few, translation b) few, transformation c) no, transformation d) no, translation 2. … Read more
This set of Genetic Engineering Questions and Answers for Campus interviews focuses on “Synthesis of Proteins and Translation In Vitro”. 1. Little quantities of radiolabelled proteins are required for which of the following? a) co or post translational targeting b) modification of proteins c) both co or post translational targeting and modification of proteins d) … Read more
This set of Genetic Engineering Multiple Choice Questions & Answers (MCQs) focuses on “Synthesis of RNA”. 1. Cloning vectors designed for the purpose of synthesis of RNA and proteins are known as ___________ a) Cloning vectors b) RNA vectors c) Bacteriophage vectors d) Expression vectors 2. For studying processes such as splicing and cleavage, RNA … Read more
