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Genetic Engineering Multiple Choice Questions | MCQs | Quiz

Genetic Engineering Interview Questions and Answers
Practice Genetic Engineering questions and answers for interviews, campus placements, online tests, aptitude tests, quizzes and competitive exams.

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•   Endonucleases - 1
•   Endonucleases - 2
•   Polymerases
•   Exonucleases
•   Ligation Categories
•   Ligation Enzymes
•   Transformation
•   Plasmid DNA Purification
•   Gel Electrophoresis - 1
•   Gel Electrophoresis - 2
•   Gel Electrophoresis - 3
•   Basic Techniques
•   Primers
•   Applications
•   Precautions & Drawbacks
•   PCR Modifications - 1
•   PCR Modifications - 2
•   Plasmid Cloning - 1
•   Plasmid Cloning - 2
•   Vectors
•   Transformation & Hosts
•   Adaptors & Cassettes
•   BAC Vector & M13 - 1
•   BAC Vector & M13 - 2
•   Lambda Bacteriophage-1
•   Lambda Bacteriophage-2
•   Lambda Cloning - 1
•   Lambda Cloning - 2
•   Lambda ZAP & Cosmids
•   Bacteriophage Mu & Pi
•   Genomic Libraries
•   CDNA Libraries - 1
•   CDNA Libraries - 2
•   Specialized Libraries - 1
•   Specialized Libraries - 2
•   Database Screening - 1
•   Database Screening - 2
•   Vivo Screening
•   Vitro Coding
•   Reporter Genes
•   Insertions & Deletions
•   Mutagenesis
•   Mutated Sequences
•   Gene Disruption
•   Gene Silencing
•   Cre Lox Excision
•   RNA Synthesis
•   Vitro Proteins
•   Vivo Expression
•   Promoters
•   Fusion Proteins
•   Proteins & Optimization
•   Gene Function & Trapping
•   Bacteria
•   Markers
•   Expression System
•   Other Systems
•   Algae
•   Vascular Plants
•   Organelle Transformation
•   Cells & Bacillovirus
•   Drosophila
•   Intact Organisms - 1
•   Intact Organisms - 2
•   Transgenic Organsims
•   Applications

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Genetic Engineering Questions and Answers – Enzymes for Ligation

Posted on May 26, 2017 by Manish

This set of Genetic Engineering Multiple Choice Questions & Answers (MCQs) focuses on “Enzymes for Ligation”.

1. Enzyme commonly used for carrying out ligation reaction is:
a) Transferase
b) Reverse transciptase
c) Ligase
d) DNase
View Answer

Answer: c
Explanation: Ligase is the enzyme which is commonly used for carrying out the ligation reaction. They can either be obtained from E. coli or from cells that have been infected by virus.
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2. Which of the following statements is correct with respect to T4 DNA ligase?
a) It can carry out only blunt ended ligations
b) It doesn’t requires ATP
c) It requires a phosphate group at 3’ end and a hydroxyl group at 5’ end for the molecule to be joined
d) It is obtained from T4 bacteriophage upon infection by E. coli
View Answer

Answer: d
Explanation: T4 DNA ligase is obtained from T4 bacteriophage upon infection by E. coli. It carries out ligation both in blunt ended and sticky ended molecules and requires ATP. It requires a phosphate group at 5’ end and a hydroxyl group at 3’ end.

3. If blunt ended ligations are to be carried out. Which of the following enzymes can be used?
a) E. coli DNA ligase
b) T4 DNA ligase
c) Both of these enzymes act equally in carrying out blunt ended ligations
d) None of them is able to carry out blunt ended ligations
View Answer

Answer: b
Explanation: If blunt ended ligations are to be carried out, T4 DNA ligase should be used. E. coli DNA ligase is unable to carry out blunt ended ligation.

4. E. coli DNA ligase doesn’t requires a cofactor. The statement is true or false?
a) True
b) False
View Answer

Answer: b
Explanation: The E. coli DNA ligase requires a cofactor nicotamine adenine dinucleotide (NAD), along with 5’ phosphate group and 3’ hydroxyl group.
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5. Mechanism of ligation for both T4 DNA ligase and E. coli DNA ligase makes use of Adenosine Monophosphate (AMP). Which of the steps is involved in the ligation mechanism?
a) AMP is added to the 5’ phosphate of one of the DNA molecule
b) It leads to liberation of pyrophosphate from NAD and nicotinamide mononucleotide from ATP
c) The AMP is further displaced by an electrophilic attack
d) The AMP is further displaced by nucleophilic attack by 3’ hydroxyl of the same DNA molecule
View Answer

Answer: a
Explanation: AMP is added to the 5’ phosphate of one of the DNA molecule. It leads to liberation of pyrophosphate from ATP and nicotanamide mononucleotide from NAD. It is further displaced by nucleophilic attack by 3’ hydroxyl of the other DNA molecule.

6. Topoisomerase is also an enzyme which is used for carrying out ligation. The correct statement for topoisomerase is?
a) They act only on double stranded molecules
b) They alter the degree of supercoiling of DNA molecules
c) They are less effective than conventional DNA ligase
d) There are three types of topoisomerases
View Answer

Answer: b
Explanation: Topoisomerases alter the degree of supercoiling of both single and double stranded DNA molecules. There are two types of topoisomerases, I and II. I is responsible for altering single stranded molecules and type II for altering double stranded molecules. They are more effective than conventional DNA ligase.

7. Mobile genetic elements can be transferred from one DNA portion to another. The enzyme carrying out this is:
a) Ligase
b) Transciptase
c) Transposase
d) Endonuclease
View Answer

Answer: c
Explanation: Transposase is used for transferring mobile genetic elements from one portion to another. They are useful in inserting origin of replication or anti-biotic resistance genes.
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8. Phage based recombination systems are used for:
a) cleaving the molecules at specific sites
b) adding the molecules at specific sites
c) breakage and rejoining the molecules at specific sites
d) breakage at random sites
View Answer

Answer: c
Explanation: Phage based recombination systems are used for breaking and rejoining the moleculesat specific sites and not at random sites.

9. Bacteriophage lambda is having a phage recombination system. Following are the characteristics of this system:
a) It is used for inserting phage genome into the bacterium
b) It is used for inserting bacterial genome into the phage
c) The specific site in bacteria is attB and that in phage is attP
d) The specific sites in both of them are called as attP
View Answer

Answer: a
Explanation: The phage genome is inserted into the bacterium. The site for insertion at the phage genome is attP and that in the case of bacterium is attB.

10. Ligation enzymes are used for ligating newly synthesized okazaki fragments. What holds true for okazaki fragments?
a) Okazaki fragments are short fragments of DNA formed on leading strand
b) Okazaki fragments are large fragments of DNA formed on lagging strand
c) Okazaki fragments are short fragments of DNA formed on lagging strand
d) Okazaki fragments are large fragments of DNA formed on leading strand
View Answer

Answer: c
Explanation: Okazaki fragments are short DNA fragments on the lagging template strand during replication. They are complementary to the lagging strand.

Sanfoundry Global Education & Learning Series – Genetic Engineering.
To practice all areas of Genetic Engineering, here is complete set of 1000+ Multiple Choice Questions and Answers.

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« Genetic Engineering Questions and Answers – Categories of Ligation Reaction
Instrumentation Transducers Questions and Answers – Measurement of Current »
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Deep Dive @ Sanfoundry:

  1. Geotechnical Engineering Questions and Answers
  2. Biochemistry Questions and Answers
  3. Engineering Chemistry Questions and Answers
  4. Genetic Engineering Questions and Answers
  5. Genetic Engineering Questions and Answers – Bacteriophage-Mu and Bacteriophage-Pi
  6. Genetic Engineering Questions and Answers – cDNA Libraries, Polyadenylated RNA and cDNA Synthesis-02
  7. Genetic Engineering Questions and Answers – Genomic Libraries
  8. Genetic Engineering Questions and Answers – BAC Vector, M13 and its Derivatives-01
  9. Biochemistry Questions and Answers – DNA Replication
  10. Genetic Engineering Questions and Answers – Bacteriophage Lambda-2
Manish Bhojasia, a technology veteran with 20+ years @ Cisco & Wipro, is Founder and CTO at Sanfoundry. He is Linux Kernel Developer and SAN Architect and is passionate about competency developments in these areas. He lives in Bangalore and delivers focused training sessions to IT professionals in Linux Kernel, Linux Debugging, Linux Device Drivers, Linux Networking, Linux Storage & Cluster Administration, Advanced C Programming, SAN Storage Technologies, SCSI Internals and Storage Protocols such as iSCSI & Fiber Channel. Stay connected with him below:
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