This set of Genetic Engineering Multiple Choice Questions & Answers (MCQs) focuses on “Algae”.
1. Chlamyodomonas reinhardtii is a green _________ algae.
d) either unicellular or di-cellular
Explanation: Chlamyodomonas reinhardtii is a green, unicellular algae. It has been used as a model system and studies have been carried out.
2. Chlamyodomonas genome is _______ rich.
c) Only A
d) Only C
Explanation: Chlamyodomonas genome is very GC rich. Hence, heterologous genes can’t be expressed until they come from a GC rich genome.
3. Integration of DNA into the Chlamydomonas genome is via homologous recombination.
Explanation: Integration of DNA into the Chlamydomonas genome is via non-homologous recombination. And because of this gene disruption can’t be carried out efficiently.
4. Transformation of Chlamydomonas is most efficient in which method?
a) Lithium acetate method
c) Protoplast based method
d) Both electroporation and protoplast based method
Explanation: Transformation of Chlamydomonas is most efficient in the protoplast method. In this autolysin is used in order to carry out cell wall degradation.
5. OEE1 gene encodes a component of _________
a) photosystem I
b) photosystem II
c) both photosystem I and II
d) which neither belongs to photosystem I nor II
Explanation: OEE1 gene encodes a component which is a component of photosystem II. It allows mutant cells lacking that polypeptide to grow photoautotrophically.
6. Homologue of yeast ARG4 in Chlamydomonas is __________
Explanation: ARG4 is present in yeast and is used for the production of arginine and as a selectable marker. Homologue of yeast ARG4 in Chlamydomonas is ARG7.
7. cw15 is a mutant strain, it ________ and thus called as ________
a) lacks cell wall, natural protoplast
b) lacks cell wall, artificial protoplast
c) has cell wall, artificial protoplast
d) has cell wall, natural protoplast
Explanation: cw15 is a mutant strain. It lacks a cell wall and thus it is called as natural protoplast. It is so because protoplasts are those structures which lack a cell wall.
8. Genetic manipulation of Volvox carteri is carried out using nitrogen reducatse or antibiotic resistance.
Explanation: Genetic manipulation of green algae such as Volvox carteri is carried out by using nitrogen reductase or antibiotic resistance. They act as selectable markers.
9. For Chlorella, which method is used for DNA uptake?
b) Direct uptake of naked DNA
d) Chemically induced uptake by protoplast
Explanation: For Chlorella biolistic transformation is used. And apart from it, chemically induced uptake of DNA by protoplast is also used.
10. Members of genera Haematococcus are used for production of ____________
d) carotenoid and xanthenoid both
Explanation: Members of genera Haematococcus and Dunaliella are of economic importance and are used for the production of carotenoids.
Sanfoundry Global Education & Learning Series – Genetic Engineering.
To practice all areas of Genetic Engineering, here is complete set of 1000+ Multiple Choice Questions and Answers.