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Genetic Engineering Multiple Choice Questions | MCQs | Quiz

Genetic Engineering Interview Questions and Answers
Practice Genetic Engineering questions and answers for interviews, campus placements, online tests, aptitude tests, quizzes and competitive exams.

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•   Endonucleases - 1
•   Endonucleases - 2
•   Polymerases
•   Exonucleases
•   Ligation Categories
•   Ligation Enzymes
•   Transformation
•   Plasmid DNA Purification
•   Gel Electrophoresis - 1
•   Gel Electrophoresis - 2
•   Gel Electrophoresis - 3
•   Basic Techniques
•   Primers
•   Applications
•   Precautions & Drawbacks
•   PCR Modifications - 1
•   PCR Modifications - 2
•   Plasmid Cloning - 1
•   Plasmid Cloning - 2
•   Vectors
•   Transformation & Hosts
•   Adaptors & Cassettes
•   BAC Vector & M13 - 1
•   BAC Vector & M13 - 2
•   Lambda Bacteriophage-1
•   Lambda Bacteriophage-2
•   Lambda Cloning - 1
•   Lambda Cloning - 2
•   Lambda ZAP & Cosmids
•   Bacteriophage Mu & Pi
•   Genomic Libraries
•   CDNA Libraries - 1
•   CDNA Libraries - 2
•   Specialized Libraries - 1
•   Specialized Libraries - 2
•   Database Screening - 1
•   Database Screening - 2
•   Vivo Screening
•   Vitro Coding
•   Reporter Genes
•   Insertions & Deletions
•   Mutagenesis
•   Mutated Sequences
•   Gene Disruption
•   Gene Silencing
•   Cre Lox Excision
•   RNA Synthesis
•   Vitro Proteins
•   Vivo Expression
•   Promoters
•   Fusion Proteins
•   Proteins & Optimization
•   Gene Function & Trapping
•   Bacteria
•   Markers
•   Expression System
•   Other Systems
•   Algae
•   Vascular Plants
•   Organelle Transformation
•   Cells & Bacillovirus
•   Drosophila
•   Intact Organisms - 1
•   Intact Organisms - 2
•   Transgenic Organsims
•   Applications

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Genetic Engineering Questions and Answers – Expression of Coding Section in-Vitro

Posted on May 26, 2017 by Manish

This set of Genetic Engineering Multiple Choice Questions & Answers (MCQs) focuses on “Expression of Coding Section In-Vitro”.

1. If expression of the coding sequence is to be carried in the vitro, then which of the statement holds true?
a) The extract in which the host DNA constructs are incubated is capable of translation only
b) The extract in which the host DNA constructs are incubated is capable of transcription only
c) The extract is made up of lysate of E. coli cells, containing RNA polymerase, ribosomes, tRNAs etc.
d) Non radiolabelled amino acids are used
View Answer

Answer: c
Explanation: If expression of the coding sequence is to be carried in vitro, then the extract in which host DNA constructs are incubated is capable of both transcription and translation. The extract is made up of lysate of E. coli cells, containing RNA polymerase, ribosomes, tRNAs etc. Radiolabelled amino acids are used and thus detection becomes easier.
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2. If translation and transcription are taking place together, the reaction is called as ______ and if they are carried out separately, it is called as _______
a) coupled, linked
b) linked, coupled
c) grouped, separate
d) grouped, individual
View Answer

Answer: a
Explanation: If translation and transcription are carried out together, it is known as coupled reaction. If they are carried out separately then they are known as linked reactions.

3. If translation and transcription are carried out separately, then what is added to carry out translation?
a) DNA polymerase
b) RNA polymerase
c) Taq polymerase
d) Tfl polymerase
View Answer

Answer: b
Explanation: The added RNA polymerase carries out the translation in a separate reaction by the use of lysate of wheat gram cells.

4. If the screening of the DNA constructs is carried out in pooled batches then it takes less time. Is the given statement true or false?
a) True
b) False
View Answer

Answer: a
Explanation: If the screening of DNA constructs is carried out in pooled batches then less time is taken. Once the pooled batch is identified with recombinants then each member is screened.
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5. Choose the incorrect statement for open reading frame.
a) It is helpful in deciding whether the clone is correct or not
b) It consists of an initiation codon
c) It consists of termination codon
d) The presence of introns doesn’t affects the open reading frame
View Answer

Answer: d
Explanation: Open reading frame is very important in deciding whether the clone is correct or not. It consists of an initiation codon, followed by a stretch of DNA and termination codon. The presence of introns the open reading would be affected and makes detection difficult.

6. Introns are ________ in nature and the sequence is _______ than exons.
a) coding, less random
b) non-coding, less random
c) non-coding, more random
d) coding, more random
View Answer

Answer: c
Explanation: Introns are non-coding in nature and thus the sequence is having a more random base composition than exons. Exons are coding sequences.

7. If the genome size is small, then sequencing of the entire genome is:
a) easy
b) difficult
c) independent of the size of genome
d) not possible
View Answer

Answer: a
Explanation: If the genome size is small, then the sequencing of entire genome is carried out easily. Hence, direct sequencing is carried out if the genome size is small.
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8. Choose the correct statement with respect to bacterial genome.
a) They are easy to sequence
b) They are difficult to sequence
c) Methods such as transcription and translation are satisfactory
d) Only transcription can be carried out
View Answer

Answer: b
Explanation: Bacterial genome is of large size and thus it is difficult to carry out sequencing. Methods such as transcription and translation are unsatisfactory.

9. For identification of genes that are expressed under special conditions, _______ libraries are used.
a) cDNA
b) genomic
c) subtractive
d) shelf
View Answer

Answer: c
Explanation: If genes are expressed under special conditions then specialized libraries should be used. These specialized libraries are subtractive. In this, driver and tracers are used.

10. In ______ organisms, firstly the gene is looked into model organisms.
a) eukaryotic
b) prokaryotic
c) both eukaryotic and prokaryotic
d) large sized
View Answer

Answer: a
Explanation: In eukaryotic organisms, firstly the gene is looked into the model organism. In the model organism, the genome size is small. Eukaryotic organisms generally have a varying size of genome.

Sanfoundry Global Education & Learning Series – Genetic Engineering.
To practice all areas of Genetic Engineering, here is complete set of 1000+ Multiple Choice Questions and Answers.

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