Genetic Engineering Questions and Answers – Cultured Cells And Bacillovirus

This set of Genetic Engineering Problems focuses on “Cultured Cells And Bacillovirus”.

1. Autographa californica nuclear polyhedrois virus (AcNPV) is commonly used bacilovirus for infecting cultured cells. The virus is ________ and _______
a) single stranded, linear
b) double stranded, linear
c) double stranded, circular
d) single stranded, circular
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2. DNA replication of the AcNPV takes 6 hours after infection. After how many hours of infection new virus particles are produced by budding?
a) 8 hrs
b) 10 hrs
c) 12 hrs
d) 16 hrs
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3. The virus particles are held together by polyhedron and p10 protein.
a) True
b) False
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4. The polyhedrin and p10 protein are produced in ____________ amount and other genes are expressed in _________ amount.
a) low, high
b) high, high
c) high, low
d) low, low
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5. Expression of proteins using nuclear polyhedrosis viruses is advantageous because it gives _______ protein yields and post-translational modifications are _________
a) high, not possible
b) high, possible
c) low, possible
d) low, not possible
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6. The size of the viral genome is large and thus poses a problem. This problem can be resolved by using ____________
a) transfer vector
b) co-integration plasmid
c) hybrid vector
d) fusion vector
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7. The gene of interest is inserted into transfer vector and then further into viral genome leading to the formation of modified virus. How can plaques from modified virus differentiated from plaques from wild-type virus?
a) Chemically
b) Visually
c) On the basis of magnetic properties
d) On the basis of longeitivity of plaques
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8. If the DNA is linearized, what is the effect on the recombination frequency?
a) The recombination frequency decreases
b) The recombination frequency increases
c) The recombination may increase or decrease depending on the amount of DNA
d) There is no effect on the recombination frequency
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9. The recombinant virus can be propagated in E.coli and this is referred to as bacmid.
a) True
b) False
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10. For the generation of recombinant baculovirus, recombinants can be selected by ____________
a) blue-white screening
b) antibiotic resistance
c) either antibiotic resistance or blue white screening
d) radioactivity
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Sanfoundry Global Education & Learning Series – Genetic Engineering.

To practice all areas of Genetic Engineering Problems, here is complete set of 1000+ Multiple Choice Questions and Answers.

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