This set of Genetic Engineering Multiple Choice Questions & Answers (MCQs) focuses on “Applications of Genetic Engineering”.
1. Amplification of specific region can be done by using primers for specific regions. If the PCR product is ______ and is in sufficient quantity, then sequence can be determined ________
a) non-specific, directly
b) non-specific, indirectly or directly
c) specific, directly
d) specific, indirectly
Explanation: Amplification of specific region can be done by using primers for specific regions. If the PCR product is specific, it means that only a single band is obtained in gel electrophoresis and is in sufficient quantity then the sequence can be determined directly.
2. Which of the following is not suitable if the PCR product is non-specific?
a) Adjusting the concentration of magnesium ions
b) Increasing annealing temperature
c) Using touchdown PCR
d) Using inverse PCR
Explanation: In case, products are non-specific then sequence can’t be known directly. Optimization of PCR should be carried out and various other strategies should be used such as adjusting the concentration of magnesium ions, increasing annealing temperature or using touch-down PCR.
3. The disadvantage in the approach based on using PCR is that there is no permanent record until some of the bacterial cells are preserved. Is the given statement true or false?
Explanation: The disadvantage in this approach is that there is no permanent record until some of the bacterial cells are preserved. If enough bacteria are there initially then genomic library can be constructed.
4. How many approaches are there in order to clone the complete genome?
Explanation: There are basically two approaches in order to clone the complete genome. In the first approach systematic cloning is cloned is carried out. The second approach is based on cloning overlapping fragments at random.
5. If a full proteomic analysis of growth medium is carried out and is combined with ______ genome sequence, genes for other _____ proteins are also obtained.
a) partial, defensive
b) partial, secreted
c) complete, defensive
d) complete, secreted
Explanation: If full proteomic analysis of growth medium is carried out and is combined with complete genome sequence, genes for other secreted proteins can also be obtained.
6. If a putative protein sequence is cloned in an expression vector and the expressed protein is not showing protease activity, then which of the following is not correct?
a) The protein is not protease
b) The protein can be incorrectly folded which can block the protease activity
c) There might be some other cofactor required for protease activity
d) The most commonly used expression system is E.coli
Explanation: If the putative protein sequence is cloned and the expressed protein is not showing protease activity, it is not necessary that it is not protease. Suppression of protease activity can be because of incorrect folding or that some cofactor is required for protease activity. The most commonly used expression system is E.coli.
7. For getting large amount of proteins to crystallize, which of the following should be used as an expression system?
a) Bacterial system
b) Yeast systems
c) Eukaryotic systems
d) Both eukaryotic and bacterial systems can be used
Explanation: The system to be used for getting large amounts of proteins to crystallize can be either bacterial or eukaryotic. It depends on the source of the gene and whether post-translational modification is necessary or not.
8. If a mutation perturbs the structure, then stability and folding is not affected. Is the given statement true or false?
Explanation: At times mutated proteins are not expressed well. Mutation perturbs a structure at times and it affects the stability and folding of the protein.
9. The RNA level ______ the steady-state level of the corresponding protein directly and the post-translational modification of the protein ______.
a) reflects, can be determined
b) reflects, can’t be determined
c) doesn’t necessarily reflects, can be determined
d) doesn’t necessarily reflects, can’t be determined
Explanation: The RNA level doesn’t necessarily corresponds to the steady level of corresponding protein directly and the post-translational modification or location of the protein can’t be determined.
10. For a convenient transformation system, _____ can be used for gene silencing.
a) antisense RNA
b) transposon insertion
c) either antisense RNA or transposon insertion
d) transposon insertion followed by antisense RNA
Explanation: If a convenient transformation system is used, antisense RNA can be used for gene silencing. If the transformation system is not convenient then transposon insertion is used for silencing.
Sanfoundry Global Education & Learning Series – Genetic Engineering.
To practice all areas of Genetic Engineering, here is complete set of 1000+ Multiple Choice Questions and Answers.