Genetic Engineering Questions and Answers – Removal and Introduction of Restriction Sites and Generation of Insertions and Deletions

This set of Genetic Engineering Question Bank focuses on “Removal and Introduction of Restriction Sites and Generation of Insertions and Deletions”.

1. Direct alteration of particular parts of protein as a way of probing the relationship between structure and function is termed as:
a) genetic engineering
b) protein engineering
c) alteration of protein function
d) structure engineering
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2. At times, the gene which is cloned is not well known for the protein encoded by it. To access the function, the endogenous gene for the mutant strain is inactivated. This technique is called as ___________
a) reverse genetics
b) protein engineering
c) mutation
d) location of function
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3. Approaches for creating mutations can be divided into how many types?
a) 1
b) 2
c) 3
d) 4
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4. In the case of deletion of a restriction site, it should be cleaved with the same restriction enzyme.
a) True
b) False
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5. If there are multiple restriction sites for an enzyme in a given molecule, digestion is carried out in many steps. The initial digestion should be?
a) complete
b) partial
c) carried out by a different enzyme then that the restriction site
d) carried out by a mixture of restriction enzymes
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6. If a functional gene is disrupted while disrupting a restriction site _______ is created.
a) frameshift mutation
b) point mutation
c) either of the mutations
d) any other kind of mutation
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7. Additional restriction sites can be introduced near the existing restriction site by mutagenesis.
a) True
b) False
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8. Restriction site can also be introduced by oligonucleotide directed mutagenesis of a region that is _______ and ______ to the original restriction site.
a) not only similar, also exactly same
b) similar, not same
c) not similar, is entirely different
d) different, near
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9. Small deletions at a restriction site can be generated by cutting and degrading the ________ with an exonuclease.
a) double stranded ended
b) circular molecules
c) single stranded ends
d) supercoiled molecules
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10. When a series of deleted regions are replaced by some other DNA fragment of equal length, then it is known as:
a) linker scanning
b) generation of deletions
c) mutation
d) replacement
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Sanfoundry Global Education & Learning Series – Genetic Engineering.

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