This set of Cytogenetics Questions and Answers for Freshers focuses on “Post-Transcriptional Modification – 2”. 1. In an experiment, you are trying to pull down the site where guanyl transferase binds by co-immunoprecipitation. You run a gel and autoradiograph to see a band denoting GSK-RNA pol II (phosphorylated). What will you see when you perform … Read more
This set of Cytogenetics Multiple Choice Questions & Answers (MCQs) focuses on “Post-Transcriptional Modification – 1”. 1. If the events in post transcriptional modification are listed as- i) Polyadenylation ii) capping iii) splicing What would be the correct sequence? a) i->ii->iii b) iii->ii->i c) i->iii->ii d)ii->i->iii 2. Which RNA transcript would be capped by the … Read more
This set of Cytogenetics Multiple Choice Questions & Answers (MCQs) focuses on “Transcription in Eukaryotes : Chromatin Remodelling”. 1. In which of the following chromosome compaction is absent? a) Cellulomonas flavigena b) Allium sativum c) Panthera tigris d) Saccharomyces cerevisiae 2. Histones are __________ a) Neutral b) Positively charged c) Negatively charged d) Neutral with … Read more
This set of Cytogenetics Assessment Questions and Answers focuses on “Transcription in Eukaryotes : Gene Silencing at Telomere”. 1. RAP1 has a ___________________ a) Positive effect on transcription b) No effect on transcription c) Negative effect on transcription d) Immensely positive effect on transcription 2. We know that loss of DNA bases is highly mutagenic. … Read more
This set of Cytogenetics Multiple Choice Questions & Answers (MCQs) focuses on “Transcription in Eukaryotes : Activators and Repressors”. 1. Promoter proximal elements is positioned ________________ a) Outside the core promoter b) Downstream to start site c) Within the enhancer d) Just before the start site 2. TATA box in eukaryotes would be present in … Read more
This set of Basic Cytogenetics Questions and Answers focuses on “Transcription in Eukaryotes : Transcription Factors”. 1. Which of this is a cis binding element? a) TAF b) TBP c) TFIIF d) TFIIH 2. You wanted to study the assembly of preinitiation complex in eukaryotes. This is by the means of protein-protein interaction. Which of … Read more
This set of Cytogenetics Multiple Choice Questions & Answers (MCQs) focuses on “Transcription in Eukaryotes : RNA Polymerases”. 1. Prokaryotes have 1 RNA polymerase. How many RNA polymerases are there in eukaryotes? a) 2 b) 3 c) 4 d) None 2. You isolate the nucleolus from the nucleus and performed a density gradient centrifugation with … Read more
This set of Cytogenetics Multiple Choice Questions & Answers (MCQs) focuses on “Operons : Trp”. 1. Which of these operons is anabolic? a) Lac b) Ara c) Trp d) Phe 2. Which of these systems give the best mode for turning trp operon off? a) Repressor b) Attenuator c) Repressor with a downstream poly A … Read more
This set of Cytogenetics Multiple Choice Questions & Answers (MCQs) focuses on “Operons : Lac and Ara”. 1. You want your bacterial culture to grow well so you made an enriched media with all forms of carbohydrates. Which of this carbohydrate should you restore first if you want the culture to keep growing at the … Read more
This set of Cytogenetics Problems focuses on “Transcription in Prokaryotes : Sigma Factor”. 1. If you study T4 bacteriophage transcription with holoenzyme which genes will you expect to be transcribed? a) Immediate early b) Early c) Delayed d) Late 2. You transcribe a gene and obtain mRNA. To detect which strand is transcribed you replicate … Read more
