This set of Gene Manipulation Multiple Choice Questions & Answers (MCQs) focuses on “Basic Laboratory Techniques – 1”.
1. When was the initial impetus for gene manipulation observed?
Explanation: The initial impetus for gene manipulation in vitro came about in the early 1970s with the simultaneous development of related techniques.
2. Which of the following is the rarest event in transformed cells?
a) DNA integration
b) DNA uptake
Explanation: The exogenous DNA may not be maintained in the transformed cells. If the exogenous DNA is integrated into the host genome it is a rare event.
3. The exogenous DNA is subsequently lost, if it does not integrate into the host genome.
Explanation: If the exogenous DNA fails to be integrated, it will probably be lost during subsequent multiplications of the host cells.
4. In order to be replicated, DNA molecules must contain _________
Explanation: In order to be replicated DNA molecules must contain an origin of replication, and in bacteria and viruses there is usually one per genome.
5. Fragments of DNA are not __________
Explanation: Fragments of DNA are not replicons and in the absence of replication, will be diluted out of their host cells. Replicons are capable of replicating by themselves.
6. Even if a DNA molecule contains __________ it may not function in a host cell.
a) Origin of replication
Explanation: It should be noted that even if a DNA molecule contains an origin of replication, it may not function in the host cell. Replicons are capable of replicating by themselves.
7. Cloning vehicles are actually ________
Explanation: If fragments of DNA are not replicated. The obvious solution is to attach them to a suitable replicon. Such replicons are called vectors or cloning vehicles.
8. ___________ also permits the easy bulk preparation of the foreign DNA sequence free from host-cell DNA.
Explanation: The vector does not only provide the replicon function but also permits the easy bulk preparation of the foreign DNA sequence free from host cell DNA.
9. Chimeras in cloning science refer to ______________
a) Plural molecules
b) Single entity
c) Admixture of proteins
d) Composite molecule
Explanation: Composite molecules in which foreign DNA has been inserted into a vector molecule are called DNA chimeras.
10. Artificial recombinant molecules are actually chimeras.
Explanation: Composite molecules in which foreign DNA has been inserted into a vector molecule are called DNA chimeras. The construction of such recombinant molecules is termed as genetic engineering.
11. Molecular cloning is the same as ____________
a) Gene cloning
b) Vector designing
c) Protein expression
Explanation: The process of genetic engineering is also termed as molecular or gene cloning because a line of genetically identical organisms can be propagated.
12. In the cloning process which of the following is the main passenger?
a) Foreign DNA
b) Host DNA
Explanation: Although conceptually very simple, cloning of a fragment of foreign, or passenger, or target DNA in a vector demands few things.
13. Gel electrophoresis is used for _________ of cutting and joining DNA molecules.
Explanation: The cutting and joining reactions of DNA must be readily monitored. This is achieved by the use of gel electrophoresis.
14. When are the first cloning experiments observed?
Explanation: The necessary techniques became available at about the same time and led to many cloning experiments, the first of which were observed in 1972 by Jackson and in 1973 by Lobban and Kaiser.
15. Gel electrophoresis separates the molecules based on _________
Explanation: The technique of gel electrophoresis is used to separate different nucleic acid molecules based on their size. This technique superseded the earlier use of sucrose gradients.
Sanfoundry Global Education & Learning Series – Vector Biology & Gene Manipulation.
To practice all areas of Vector Biology & Gene Manipulation, here is complete set of 1000+ Multiple Choice Questions and Answers.