Vector Biology Questions and Answers – Vectors for Mammals – 9

This set of Vector Biology Multiple Choice Questions & Answers (MCQs) focuses on “Vectors for Mammals – 9”.

1. Baculovirus vectors cannot co-express multiple transgenes.
a) True
b) False
View Answer

Answer: b
Explanation: Baculovirus vectors can co-express multiple transgenes, and thus modify the glycosylation process in host cell line by expressing appropriate glycosylation enzymes along with the transgene of interest.

2. Polyhedrin gene replacement vectors are most popular due to __________
a) Wide host range
b) Recombinant protein production
c) Gene Cloning
d) Lytic cycle
View Answer

Answer: b
Explanation: Polyhedrin gene replacement vectors are the most popular due to the high level of recombinant protein that can be expressed.

3. Recombinant plaques appear ___________ when viewed under oblique light source.
a) Clear
b) White
c) Blue
d) Red
View Answer

Answer: a
Explanation: The occlusion bodies produced by wild-type viruses cause the microscopic viral plaques appear opalescent if viewed under oblique light source, while recombinant plaques appear clear.
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4. Blue-white screening in Baculovirus vectors can be facilitated by ____________
a) Ampicillin resistance gene
b) Toluene resistance gene
c) lacZ’ gene
d) Rep gene
View Answer

Answer: c
Explanation: Insertion of the E.coli lacZ gene in frame into the polyhedron coding regions allow blue white screening of the recombinants.

5. How can the recombinants be identified if LacZ’ is substituted by GFP?
a) Blue white screening
b) UV light exposure
c) Plaque color screening
d) Lactose breakdown
View Answer

Answer: b
Explanation: Substitution of lacZ with the gene for green fluorescent protein allows the rapid identification of recombinants by exposing the plaques to UV light.

6. With respect to polyhedrin promoter, where is the transgene inserted in a Baculovirus vector?
a) Upstream
b) Downstream
c) 4 nucleotides away
d) 3 nucleotides away
View Answer

Answer: b
Explanation: The construction of baculovirus expression vectors involves inserting the transgene downstream of the polyhedrin promoter.

7. What is a Bacmid?
a) Hybrid replicon
b) AAV
c) Baculovirus
d) Phage
View Answer

Answer: a
Explanation: In the bacterial system, marketed by Gibco-BRL, the baculovirus genome is engineered to contain an origin of replication from the E.coli F plasmid.
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8. The target site for which transposon is present in a Bacmid?
a) Tn2
b) Tn4
c) Tn7
d) Tn9
View Answer

Answer: c
Explanation: The hybrid replicon, bacmid also contains the target site for the transposon Tn7, inserted in-frame within the lacZ gene, which is itself downstream of the polyhedrin promoter.

9. Other than delivering foreign genes into mammalian cells, Baculoviruses can also be used for the delivery of __________
a) Bacteria
b) Other cells
c) Recombinant molecules
d) Viruses
View Answer

Answer: d
Explanation: Baculoviruses are useful not only for the delivery of foreign genes into mammalian cells but also for the delivery of other viruses.
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10. Hepatitis C Virus does not infect cultured cells.
a) True
b) False
View Answer

Answer: a
Explanation: Hepatitis C Virus does not infect cultured cells, but a hybrid baculovirus containing the entire HCV genome can initiate an HCV infection.

11. How can HCV infection be initiated in cultured cells?
a) By increasing the genome size
b) Splicing unnecessary genes
c) Using hybrid vectors
d) In vitro mutation
View Answer

Answer: c
Explanation: Hepatitis C Virus does not infect cultured cells, but a hybrid baculovirus containing the entire HCV genome can initiate an HCV infection.

12. Fd-Adv Vectors contain no ___________
a) Capsid
b) Head
c) Genes
d) Origin
View Answer

Answer: c
Explanation: The highest capacity amplicons or fully deleted adenoviruses (FD-Adv) contain no viral genes at all, only elements required for replication and packaging.

13. The Herpesviruses are _______________
a) Small double-stranded
b) Large double-stranded
c) Small single-stranded
d) Large single-stranded
View Answer

Answer: b
Explanation: The herpesviruses are large double-stranded DNA viruses that include Epstein-Barr viruses and the herpes simplex viruses.

14. HSV vectors are particularly suitable for gene therapy in ________
a) Nervous system
b) Reproductive system
c) Aesthetics
d) Liver
View Answer

Answer: a
Explanation: Viral replication can occur in many cell types in a wide range of species if the genome is introduced y transfection, but HSV vectors are particularly suitable for gene therapy in the nervous system because the virus is remarkably neurotropic.

15. Retroviruses are acutely oncogenic because they carry particular genes that promote ___________
a) Cell division
b) Cell proliferation
c) Cell death
d) Phagocytosis
View Answer

Answer: a
Explanation: Retroviruses are RNA Viruses that replicate via a double-stranded DNA intermediate. Certain retroviruses are acutely oncogenic because they contain particular genes that promote host cell division.

Sanfoundry Global Education & Learning Series – Vector Biology & Gene Manipulation.

To practice all areas of Vector Biology & Gene Manipulation, here is complete set of 1000+ Multiple Choice Questions and Answers.

If you find a mistake in question / option / answer, kindly take a screenshot and email to [email protected]

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Manish Bhojasia, a technology veteran with 20+ years @ Cisco & Wipro, is Founder and CTO at Sanfoundry. He lives in Bangalore, and focuses on development of Linux Kernel, SAN Technologies, Advanced C, Data Structures & Alogrithms. Stay connected with him at LinkedIn.

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