Vector Biology Questions and Answers – Vectors for Yeast – 1

This set of Vector Biology Multiple Choice Questions & Answers (MCQs) focuses on “Vectors for Yeast – 1”.

1. Why is a eukaryotic cloning vector required?
a) Cloning ease
b) Variety
c) Availability
d) Large scale production
View Answer

Answer: d
Explanation: At times mere study of the gene is not the goal of a cloning experiment. Especially in biotechnology when the aim to obtain large amounts of product vectors and cell systems other than the prokaryotic ones are used.

2. Saccharomyces cerevisiae is an example of _________
a) Fungi
b) Yeast
c) Bacteria
d) Insect
View Answer

Answer: b
Explanation: After the extensively used E.coli in the recombinant DNA technology, next comes S.cerevisiae which is a yeast and a eukaryotic organism.

3. Plasmids are present in eukaryotes.
a) True
b) False
View Answer

Answer: a
Explanation: Plasmids are usually the extrachromosomal entities of a plasmid however a plasmid is also present in some strains of yeast.

4. The 2 micrometer circle found in yeast is a __________
a) Phage
b) Plasmid
c) Virus
d) Gene product
View Answer

Answer: b
Explanation: The 2 micrometer circle found in yeast is the only plasmid of eukaryotic origin. The discovery of this plasmid has stimulated the use of yeast as a cloning vector.

5. What is the size of 2 micrometer circle?
a) 1 kb
b) 6 kb
c) 11 kb
d) 22 kb
View Answer

Answer: b
Explanation: The 2 micrometer circle is approximately 6 kb in size, well within the range of an ideal vector. It is present in a yeast cell at a copy number of between 70 and 200.
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6. What are REP1 and REP2 genes in the plasmid used for?
a) Origin
b) Replication
c) Expression
d) Host lysis
View Answer

Answer: b
Explanation: Replication makes use of origin of replication, sever enzymes provided by the host and the REP1 and REP2 genes encoded by the plasmid.

7. Which selection system is used in a yeast plasmid recombinant?
a) Antibiotic
b) Lac
c) Auxotrophic mutant
d) cI gene
View Answer

Answer: c
Explanation: A normal yeast gene which codes for an enzyme involved in amino acid biosynthesis is incorporated into the vector. The host that is selected has a mutated copy of the incorporated plasmid gene.

8. What is an auxotrophic mutant?
a) Defective plasmid
b) Transformed cell
c) Host cell
d) Yeast cell
View Answer

Answer: c
Explanation: An auxotrophic mutant is a host cell that lacks or has a mutated copy of a gene coding for an enzyme involved in an essential amino acid biosynthesis.

9. What does the gene LEU2 code for?
a) Lactose
b) Leucine
c) Dehydrogenase
d) Hydrogenase
View Answer

Answer: c
Explanation: LEU2 is a gene coding for the enzyme beta-isopropyl-malate-dehydrogenase, one of the enzymes involved in the conversion of pyruvic acid to leucine.

10. On which medium are yeast plasmids transformed cells plated?
a) Luria Broth
b) Agar
c) Minimal
d) Nutrient
View Answer

Answer: c
Explanation: Minimal medium contains no added amino acids and therefore cells that produce all the amino acids can only grow. Transformed cells are only able to grow on minimal medium.

11. What are YEps?
a) Mutated yeast plasmids
b) Hybrid of yeast and bacteria
c) Yeast episomal plasmids
d) Transformed cells
View Answer

Answer: c
Explanation: YEps are vectors derived from yeast plasmids. Some YEps contain the entire 2 micro-meter plasmids, others just include the origin of replication.

12. Yep13 is an example of _______
a) Yeast episomal plasmid
b) Host bacterium
c) Yeast integrative plasmid
d) Bacterial plasmid
View Answer

Answer: a
Explanation: Yep13 is a yeast episomal plasmid derived from the 2 micro-meter plasmids. It includes only the original of replication from the parent plasmid.

13. What is a shuttle vector?
a) Hybrid vector
b) Mutated yeast plasmid
c) A vector that can be used with two/more systems
d) The transformed cell further used for transformation
View Answer

Answer: c
Explanation: A shuttle vector is the one that can be used for transferring genetic material to more than one type of cells; essentially a eukaryotic and a prokaryotic system.

14. Yep13 is a shuttle vector.
a) True
b) False
View Answer

Answer: a
Explanation: Yep13 includes the 2 micro-meter origin of replication, selectable LEU2 gene and the entire Pbr322 sequence. Hence it can be used for both yeast and E.coli.

15. For which of the following plasmids purification of a recombinant molecule from the transformed cell can be difficult?
a) Episomal plasmids
b) Integrative plasmids
c) Bacterial plasmid
d) All plasmids
View Answer

Answer: b
Explanation: The yeast plasmid derived integrative plasmids are extremely difficult to purify from the host cell. These plasmids integrate into the host chromosome and remain their stable.

Sanfoundry Global Education & Learning Series – Vector Biology & Gene Manipulation.

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Manish Bhojasia, a technology veteran with 20+ years @ Cisco & Wipro, is Founder and CTO at Sanfoundry. He lives in Bangalore, and focuses on development of Linux Kernel, SAN Technologies, Advanced C, Data Structures & Alogrithms. Stay connected with him at LinkedIn.

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