This set of Gene Manipulation Questions and Answers for Campus interviews focuses on “Gene Manipulation in Gram – Positive Bacteria”.
1. GC content in gram-positive bacteria is not more than _______ percent.
Explanation: In Gram-positive bacteria, the base composition of the different genomes ranges from less than 30 percent to more than 70 percent.
2. Most of the vectors used with Streptomyces are derivatives of ____________
b) B. subtilis
c) E. coli
Explanation: Most of the vectors used with Streptomyces are derivatives of endogenous plasmids and bacteriophages from other prokaryotes.
3. SCP2 is a ______ plasmid.
Explanation: Plasmid SCP2* is a derivative of a sex plasmid SCP2. Both plasmids have a size of 31.4 kilobases and are physically indistinguishable.
4. SCP is a progenitor of low-copy-number vectors.
Explanation: SCP2* exhibits a more lethal zygosis reaction. SCP2* is important because it is the progenitor of many low-copy-number, stable vectors.
5. SLP1 and pSAM2 are examples of _______________ plasmids.
a) E. coli
Explanation: SLP1 and pSAM2 are examples of Streptomyces plasmids that normally reside integrated into a specific highly conserved transfer RNA sequence.
6. PhiC31 is a ___________ phage of Streptomyces.
Explanation: The temperate phage phiC31 is the streptomycete equivalent of phage lambda and has been subjugated as a vector. PhiC31 derived vectors have upper and lower size limits for clonable fragments.
7. What is the average insert size of phiC31 vector?
a) 4 kb
b) 8 kb
c) 10 kb
d) 12 kb
Explanation: PhiC31 derived vectors have upper and lower size limits for clonable fragments with an average insert size of 8 kilobases.
8. With phage vectors, plaques can be obtained in __________
a) One night
c) One week
d) 2-3 days
Explanation: With phage vectors, there is one advantage that plaques can be obtained overnight. With plasmid vectors however one week time is utilized for sporulation.
9. Cloning in Streptomyces is done to analyze _____________ synthesis.
Explanation: A major reason for cloning in Streptomycetes is to analyze the genetics and regulation of antibiotic synthesis. Streptomycetes produce the highest number of antibiotics.
10. The high GC content of Streptomycetes affects the frequency of restriction sites.
Explanation: Streptomycete DNA has a G+C content of 70-75% and this affects the frequency of restriction sites. AT recognition sites are rare.
11. For proper ___________ analysis of a cloned gene, it must be present on the chromosome.
Explanation: Proper transcriptional analysis of a cloned gene requires that it is present on the chromosome. Whole genome sequencing has identified many genes of unknown function.
12. Luciferase is a ______________
Explanation: Fusion of genes to reporter genes such as beta-galactosidase and luciferase can be very useful for initial transcriptional studies.
13. Genomic DNA and plasmid DNA differ in their extent of ____________
Explanation: Genomic DNA and plasmid DNA differ in their extent of supercoiling, which is known to play a major role in gene expression.
14. For chromosomal integration selective ____________ has to be maintained.
d) Host range
Explanation: Chromosomal integration is the best way to circumvent supercoiling related issues. This can be achieved by integration of non-replicative plasmids, but a selective pressure has to be maintained throughout.
15. The integrative vectors for P. aeruginosa have their origin of replication derived from __________
Explanation: Reporter gene fusion should be integrated at a neutral site and this has been achieved in Pseudomonas aeruginose using novel integrative vectors that have their origin derived from PMB1.
Sanfoundry Global Education & Learning Series – Vector Biology & Gene Manipulation.
To practice all areas of Gene Manipulation for Campus Interviews, here is complete set of 1000+ Multiple Choice Questions and Answers.