This set of Gene Manipulation Multiple Choice Questions & Answers focuses on “Gene Manipulation in Fish”.
1. Gene transfer to fish is generally carried out using ____________
a) YAC vectors
Explanation: Gene transfer to fish is generally carried out by microinjection, but other methods are emerging. Fish transgenesis can be used to study gene function.
2. Zebrafish can be used to study gene function.
Explanation: Fish transgenesis can be used to study gene function and regulation, e.g. in model species such as the zebrafish and medaka.
3. Salmon and trout are ___________
b) Commercially important
c) Protein producers
d) Transgenic fish
Explanation: Model species such as Danio rerio and Oryzias latipes are used to improve the traits of commercially important species such as salmon and trout.
4. Gene transfer technology in fish has lagged behind that in _________
Explanation: Gene transfer technology in fish has lagged behind that in mammals predominantly due to lack of suitable regulatory elements to control transgene expression.
5. The first transgenic fish carried transgenes driven by _________
Explanation: The first transgenic fish carried transgenes driven by mammalian or viral regulatory elements and their performance varied considerably.
6. Attempts to express growth hormones were initially done with ____________
Explanation: Attempts to express growth hormone genes in trout initially met with little success and this may have been due to the inability of fish cells to process mammalian introns correctly.
7. Fish are advantageous assay systems because of their ____________
c) Nutritional requirements
Explanation: Fish are advantageous assay systems because of their fecundity and the fact that fertilization and development are external and the ease with which embryos can be produced.
8. How many kinds of embryos can be produced using fish for the purpose of transgenesis?
Explanation: The fertilization and development in fish are external and the ease with which haploid and uniparental diploid embryos can be produced.
9. Fish, like frogs can be used for __________ assays.
Explanation: Like frogs, the injection of DNA into fish eggs and early embryos leads to extensive replication and expression from unintegrated transgenes and hence they can be used for transient assays.
10. Transgenic fish lines are created by ___________ of DNA into the genome.
b) Partial attachment
Explanation: Some of the DNA integrates into the genome leading to germline transformation and the production of transgenic fish lines.
Sanfoundry Global Education & Learning Series – Vector Biology & Gene Manipulation.
To practice all areas of Gene Manipulation, here is complete set of 1000+ Multiple Choice Questions and Answers.