This set of Gene Manipulation test focuses on “Gene Manipulation in Fruitflies”.
1. Gene transfer to fruit flies involves microinjection of DNA into the nucleus.
Explanation: Gene transfer to fruit flies involves the microinjection of DNA into the pole plasma. P elements are used to introduce exogenous DNA.
2. P elements introduce DNA into the Drosophila _________
a) Somatic cells
d) Totipotent cells
Explanation: P elements, also known as transposons and jumping genes are used to introduce DNA into the Drosophila germline. These are transposable DNA elements.
3. P elements are highly __________
Explanation: P elements are transposable DNA elements that under certain circumstances can be highly mobile in the germline of D. melanogaster.
4. The P-M hybrid dysgenesis is a _________
Explanation: P elements cause a syndrome of related genetic phenomena called P-M hybrid dysgenesis. It occurs when males of a P strain are mated with females of an M strain.
5. The P-M hybrid dysgenesis syndrome results in abnormal _____________
Explanation: The syndrome predominantly affects the germline and induces a high rate of mutation and frequent chromosomal aberrations, resulting in an abnormal offspring.
6. Eggs permissive for P-element transposition are called __________
Explanation: P-elements are mobilized in the eggs of M-strain females. Eggs that are permissive for P-element transposition are described as M-cytotype.
7. P-element encodes a repressor of its own.
Explanation: The P-elements cause dysgenesis in crosses within P strains, because they are not mobilized in P-cytotype eggs. P-element encodes a repressor for itself which prevents transposition.
8. The P-elements have ______ base pairs inverted terminal repeats.
Explanation: The elements are characterized by perfect 31 base pairs inverted terminal repeats which are recognized by the transposase.
9. A truncated version of which enzyme can act as repressor?
Explanation: The prototype element contains a single gene, comprising four exons, encoding the transposase; a truncated version of transposase may act as a repressor.
10. Primary transcript of which enzyme is differentially spliced in germ cells and somatic cells?
Explanation: The transposase primary transcript is differentially spliced in germ cells and somatic cells, such that functional transposase is produced only in germ cells.
Sanfoundry Global Education & Learning Series – Vector Biology & Gene Manipulation.
To practice all areas of Gene Manipulation for tests, here is complete set of 1000+ Multiple Choice Questions and Answers.