Gene Manipulation Questions and Answers – Gene Manipulation in Fruitflies

This set of Gene Manipulation test focuses on “Gene Manipulation in Fruitflies”.

1. Gene transfer to fruit flies involves microinjection of DNA into the nucleus.
a) True
b) False
View Answer

Answer: b
Explanation: Gene transfer to fruit flies involves the microinjection of DNA into the pole plasma. P elements are used to introduce exogenous DNA.

2. P elements introduce DNA into the Drosophila _________
a) Somatic cells
b) Cytosol
c) Germline
d) Totipotent cells
View Answer

Answer: c
Explanation: P elements, also known as transposons and jumping genes are used to introduce DNA into the Drosophila germline. These are transposable DNA elements.

3. P elements are highly __________
a) Toxic
b) Mobile
c) Erroneous
d) Acidic
View Answer

Answer: b
Explanation: P elements are transposable DNA elements that under certain circumstances can be highly mobile in the germline of D. melanogaster.
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4. The P-M hybrid dysgenesis is a _________
a) Technique
b) Syndrome
c) Apparatus
d) Strain
View Answer

Answer: b
Explanation: P elements cause a syndrome of related genetic phenomena called P-M hybrid dysgenesis. It occurs when males of a P strain are mated with females of an M strain.

5. The P-M hybrid dysgenesis syndrome results in abnormal _____________
a) Offspring
b) Strain
c) Gene
d) Protein
View Answer

Answer: a
Explanation: The syndrome predominantly affects the germline and induces a high rate of mutation and frequent chromosomal aberrations, resulting in an abnormal offspring.
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6. Eggs permissive for P-element transposition are called __________
a) P-cytotype
b) M-cytotype
c) L-cytotype
d) K-cytotype
View Answer

Answer: a
Explanation: P-elements are mobilized in the eggs of M-strain females. Eggs that are permissive for P-element transposition are described as M-cytotype.

7. P-element encodes a repressor of its own.
a) True
b) False
View Answer

Answer: a
Explanation: The P-elements cause dysgenesis in crosses within P strains, because they are not mobilized in P-cytotype eggs. P-element encodes a repressor for itself which prevents transposition.
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8. The P-elements have ______ base pairs inverted terminal repeats.
a) 11
b) 21
c) 31
d) 41
View Answer

Answer: d
Explanation: The elements are characterized by perfect 31 base pairs inverted terminal repeats which are recognized by the transposase.

9. A truncated version of which enzyme can act as repressor?
a) Transposase
b) Polymerase
c) Nuclease
d) Ligase
View Answer

Answer: a
Explanation: The prototype element contains a single gene, comprising four exons, encoding the transposase; a truncated version of transposase may act as a repressor.
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10. Primary transcript of which enzyme is differentially spliced in germ cells and somatic cells?
a) Polymerase
b) Helicase
c) Ligase
d) Transposase
View Answer

Answer: d
Explanation: The transposase primary transcript is differentially spliced in germ cells and somatic cells, such that functional transposase is produced only in germ cells.

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To practice all areas of Gene Manipulation for tests, here is complete set of 1000+ Multiple Choice Questions and Answers.

If you find a mistake in question / option / answer, kindly take a screenshot and email to [email protected]

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Manish Bhojasia, a technology veteran with 20+ years @ Cisco & Wipro, is Founder and CTO at Sanfoundry. He lives in Bangalore, and focuses on development of Linux Kernel, SAN Technologies, Advanced C, Data Structures & Alogrithms. Stay connected with him at LinkedIn.

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