Gene Manipulation Questions and Answers – Large Scale Mutagenesis and Interference

This set of Gene Manipulation Multiple Choice Questions & Answers (MCQs) focuses on “Large Scale Mutagenesis and Interference”.

1. Mutations can be introduced by ____________
a) Cloning
b) Recombination
c) Ligation
d) Gene targeting
View Answer

Answer: d
Explanation: Mutations can be introduced into predefined genes in vivo through a process termed as gene targeting which involves homologous recombination.

2. Gene-knockout is the generation of a null-gene.
a) True
b) False
View Answer

Answer: a
Explanation: When the aim is to inactivate the target gene completely and generate a null allele, the term gene knockout is often used.

3. Embryonic stem cells are __________
a) Totipotent
b) Pluripotent
c) Dead
d) Multipotent
View Answer

Answer: b
Explanation: Embryonic stem cells can be cultured like any established cell line, but they are derived from the very early mouse embryo and are therefore pluripotent.

4. The only organism in which systematic gene targeting has been achieved is ____________
a) Mouse
b) Drosophila
c) Fish
d) Yeast
View Answer

Answer: d
Explanation: The only organism in which systematic gene targeting has been achieved is the yeast Saccharomyces Cerevisiae. Its genome contains about 6000 open reading frames.

5. The EUROFAN project involves the use of _________ generated cassettes.
a) PCR
b) Ultracentrifugation
c) Electroporation
d) Cloning
View Answer

Answer: a
Explanation: The EUROFAN project involves the use of PCR-generated cassettes in which a selectable marker is placed between 50 basepair elements.

6. Genome-wide random mutagenesis is applicable to _________
a) Fish
b) Mammals
c) All organisms
d) Bacteria
View Answer

Answer: c
Explanation: Saturation mutagenesis has been used for many years to identify mutations affecting specific crucial biological processes.

7. Insertional mutagenesis facilitates __________
a) Cloning
b) PCR
c) Recombination
d) Centrifugation
View Answer

Answer: c
Explanation: Insertional mutagenesis leaves a DNA tag in the interrupted gene, which facilitates cloning and gene identification. It is the most popular mutagenesis strategy.

8. Ribozymes are _________ molecules.
a) Toxic
b) Large
c) DNA
d) Catalytic
View Answer

Answer: d
Explanation: Ribozymes are catalytic molecules that destroy targeted MRNAs. They carry out site-specific cleavage and ligation reactions.

Sanfoundry Global Education & Learning Series – Vector Biology & Gene Manipulation.


To practice all areas of Vector Biology & Gene Manipulation, here is complete set of 1000+ Multiple Choice Questions and Answers.

If you find a mistake in question / option / answer, kindly take a screenshot and email to [email protected]

Subscribe to our Newsletters (Subject-wise). Participate in the Sanfoundry Certification contest to get free Certificate of Merit. Join our social networks below and stay updated with latest contests, videos, internships and jobs!

Youtube | Telegram | LinkedIn | Instagram | Facebook | Twitter | Pinterest
Manish Bhojasia - Founder & CTO at Sanfoundry
Manish Bhojasia, a technology veteran with 20+ years @ Cisco & Wipro, is Founder and CTO at Sanfoundry. He lives in Bangalore, and focuses on development of Linux Kernel, SAN Technologies, Advanced C, Data Structures & Alogrithms. Stay connected with him at LinkedIn.

Subscribe to his free Masterclasses at Youtube & discussions at Telegram SanfoundryClasses.