Gene Manipulation Questions and Answers – Transgenic Technology

This set of Gene Manipulation Multiple Choice Questions & Answers (MCQs) focuses on “Transgenic Technology”.

1. Transgenic technology is particularly advanced in _________
a) Plants
b) Animals
c) Mice
d) Bacteria
View Answer

Answer: c
Explanation: Transgenic technology is particularly advanced in mice, where combinations of gene targeting, site-specific recombination and inducible transgene expression make it possible.

2. Inducible transgene expression controls ______ genes.
a) 1
b) 2
c) 3
d) 4
View Answer

Answer: b
Explanation: Inducible transgene expression makes it possible to activate and inactivate both transgenes and endogenous genes in a conditional manner.

3. Gene silencing involves ___________ genes.
a) Inhibitory
b) Activator
c) Inducer
d) Carcinogenic
View Answer

Answer: a
Explanation: Gene silencing does not involve direct modification of the target gene but rather the expression of inhibitory genes whose products interfere with expression of the target.
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4. Which of the following expression allows the control of transgene expression?
a) Repressible
b) Inducible
c) Altered
d) Toxic
View Answer

Answer: b
Explanation: Inducible expression system allows transgene expression to be controlled by physical stimuli or the application of small modulators.

5. What is used to restrict transgene expression?
a) Promoter
b) Inducer
c) Silencer
d) Reporter
View Answer

Answer: a
Explanation: In both animals and plants, cell or tissue specific promoters are used to restrict transgene expression to certain areas of the organism.

6. In __________ it is useful to restrict transgene expression to mammary glands.
a) Reptiles
b) Plants
c) Xenopus
d) Mammals
View Answer

Answer: d
Explanation: In mammals, it is useful to restrict the transgene expression to mammary glands. This is done so that recombinant proteins can be recovered from milk.

7. In plants, it is useful to restrict transgene expression to seeds.
a) True
b) False
View Answer

Answer: a
Explanation: It is useful to restrict transgene expression in plants to the seeds. Seeds provide a stable environment for protein accumulation.
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8. Drosophila heat-shock promoter is an example of ___________
a) Synthetic
b) Toxic
c) Naturally occurring
d) Rare
View Answer

Answer: c
Explanation: Most cells respond to elevated temperature by synthesizing heat-shock proteins, which include molecular chaperones and other proteins.

9. Hsp70 promoter of Drosophila, _________ the gene.
a) Acidifies
b) Neutralizes
c) Inactivates
d) Activates
View Answer

Answer: c
Explanation: In transgenic flies, any gene linked to the hsp70 promoter is more or less inactive at room temperature, but high-level expression in all cells can be induced.
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10. Inducible promoters do not respond to chemicals.
a) True
b) False
View Answer

Answer: b
Explanation: Most inducible promoters used to control transgene expression respond to chemicals, which must be supplied to the transformed cells.

Sanfoundry Global Education & Learning Series – Vector Biology & Gene Manipulation.

To practice all areas of Vector Biology & Gene Manipulation, here is complete set of 1000+ Multiple Choice Questions and Answers.

If you find a mistake in question / option / answer, kindly take a screenshot and email to [email protected]

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Manish Bhojasia - Founder & CTO at Sanfoundry
Manish Bhojasia, a technology veteran with 20+ years @ Cisco & Wipro, is Founder and CTO at Sanfoundry. He lives in Bangalore, and focuses on development of Linux Kernel, SAN Technologies, Advanced C, Data Structures & Alogrithms. Stay connected with him at LinkedIn.

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