This set of Vector Biology Multiple Choice Questions & Answers (MCQs) focuses on “Single Stranded DNA Vectors Cloning – 1”.
1. Which of the following is not a filamentous coliphage vector?
a) M13
b) Lambda
c) F1
d) Fd
View Answer
Explanation: M13, F1, FD are filamentous coliphages containing a circular single-stranded DNA molecule. These have been developed as vectors because of various advantages.
2. Coliphages are single-stranded vectors.
a) True
b) False
View Answer
Explanation: M13, F1, FD are filamentous coliphages containing a circular single-stranded DNA molecule. These have been developed as vectors because of various advantages.
3. What is the average size of single-stranded vector?
a) 6400 nucleotides
b) 1200 nucleotides
c) 2500 nucleotides
d) 5500 nucleotides
View Answer
Explanation: The phage particles have dimensions 900*9 nm and contain a single-stranded circular DNA molecule, which is 6407 (M13) or 6408 (fD).
4. The complete nucleotide sequences of fD and M13 are ___ percent identical.
a) 25
b) 97
c) 40
d) 65
View Answer
Explanation: The complete nucleotide sequences of fd and M13 are available and they are 97 percent identical. The difference is mainly in isolated nucleotides.
5. The filamentous single-stranded phages infect only _________________
a) Fungi
b) Mammals
c) Plants
d) Enteric bacteria
View Answer
Explanation: The filamentous phages only infect strains of enteric bacteria harboring F pili. The adsorption site appears to be the end of the F pilus.
6. How many phage particles are released per generation upon coliphage infection?
a) 10
b) 100
c) 500
d) 1000
View Answer
Explanation: Replication of phage DNA does not result in host lysis. Infected cells grow albeit at a slower rate. Around 1000 phage particles may be released into the medium.
7. Phage DNA enters the cell by a process in which ________ and replication are tightly coupled.
a) Encapsulation
b) Decapsidation
c) Translation
d) Transcription
View Answer
Explanation: The single stranded phage DNA enters the cell by a process in which decapsidation and replication are tightly coupled. Conversion to RF takes place.
8. What is RF?
a) Plasmid
b) Coliphage
c) Replicative form
d) Hybrid vector
View Answer
Explanation: The capsid proteins enter the cytoplasmic membrane as the viral DNA passes into the cell while being converted to a double-stranded replicative form.
9. The RF multiplies ________
a) Slowly
b) Rapidly
c) Moderately
d) Does not multiply
View Answer
Explanation: The capsid proteins enter the cytoplasmic membrane as the viral DNA passes into the cell while being converted to a double-stranded replicative form. RF replicates rapidly.
10. What happens following the morphogenesis of the cell?
a) Progeny strands release
b) DNA replication
c) Host lysis
d) Degradation
View Answer
Explanation: The progeny single strands are synthesized and released from the cell as filamentous particles the following morphogenesis at the cell membrane.
11. Dominant selectable markers can be used with __________
a) Any cell type
b) Mutant cells
c) Wild-type cells
d) Recombinant cells
View Answer
Explanation: Endogenous markers are largely superseded by so-called dominant selectable markers, which confer a phenotype that is entirely novel to the cell and can hence be used in any cell type.
12. What are dominant selectable markers?
a) Drug-resistance genes
b) Inducing genes
c) Exogenous genes
d) Endogenous genes
View Answer
Explanation: The dominant selectable markers are usually drug-resistance genes of bacterial origin and transformed cell is selected on a medium that contains the drug at an appropriate concentration.
13. Methotrexate is an analog of __________
a) Aminopterin
b) Kanamycin
c) Folic acid
d) Gentamycin
View Answer
Explanation: Methotrexate is a folic acid analog, which is a competitive inhibitor of the enzyme dihydrofolate reductase (DHFR).
14. With respect to mammalian cell cloning, salmon sperm DNA can serve as a source of ____________
a) Non-specific carrier
b) Specific carrier
c) Genomic DNA
d) Plasmid DNA
View Answer
Explanation: Calcium phosphate transfection is mostly used and the specific donor DNA is often bulked with a non-specific carrier such as cleaved Salmon sperm.
15. One application in which the use of plasmid vectors is critical, in the case of mammals is ____________
a) Stable transformation
b) Transient transformation
c) Transfection
d) Transduction
View Answer
Explanation: One application in which the use of plasmid vectors is critical, in the case of mammals is a transient transformation. Here the goal is to exploit the short-term persistence of extrachromosomal DNA.
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