Vector Biology Questions and Answers – Single Stranded DNA Vectors Cloning – 1

This set of Vector Biology Multiple Choice Questions & Answers (MCQs) focuses on “Single Stranded DNA Vectors Cloning – 1”.

1. Which of the following is not a filamentous coliphage vector?
a) M13
b) Lambda
c) F1
d) Fd
View Answer

Answer: b
Explanation: M13, F1, FD are filamentous coliphages containing a circular single-stranded DNA molecule. These have been developed as vectors because of various advantages.

2. Coliphages are single-stranded vectors.
a) True
b) False
View Answer

Answer: a
Explanation: M13, F1, FD are filamentous coliphages containing a circular single-stranded DNA molecule. These have been developed as vectors because of various advantages.

3. What is the average size of single-stranded vector?
a) 6400 nucleotides
b) 1200 nucleotides
c) 2500 nucleotides
d) 5500 nucleotides
View Answer

Answer: a
Explanation: The phage particles have dimensions 900*9 nm and contain a single-stranded circular DNA molecule, which is 6407 (M13) or 6408 (fD).
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4. The complete nucleotide sequences of fD and M13 are ___ percent identical.
a) 25
b) 97
c) 40
d) 65
View Answer

Answer: b
Explanation: The complete nucleotide sequences of fd and M13 are available and they are 97 percent identical. The difference is mainly in isolated nucleotides.

5. The filamentous single-stranded phages infect only _________________
a) Fungi
b) Mammals
c) Plants
d) Enteric bacteria
View Answer

Answer: d
Explanation: The filamentous phages only infect strains of enteric bacteria harboring F pili. The adsorption site appears to be the end of the F pilus.
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6. How many phage particles are released per generation upon coliphage infection?
a) 10
b) 100
c) 500
d) 1000
View Answer

Answer: d
Explanation: Replication of phage DNA does not result in host lysis. Infected cells grow albeit at a slower rate. Around 1000 phage particles may be released into the medium.

7. Phage DNA enters the cell by a process in which ________ and replication are tightly coupled.
a) Encapsulation
b) Decapsidation
c) Translation
d) Transcription
View Answer

Answer: b
Explanation: The single stranded phage DNA enters the cell by a process in which decapsidation and replication are tightly coupled. Conversion to RF takes place.
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8. What is RF?
a) Plasmid
b) Coliphage
c) Replicative form
d) Hybrid vector
View Answer

Answer: c
Explanation: The capsid proteins enter the cytoplasmic membrane as the viral DNA passes into the cell while being converted to a double-stranded replicative form.

9. The RF multiplies ________
a) Slowly
b) Rapidly
c) Moderately
d) Does not multiply
View Answer

Answer: b
Explanation: The capsid proteins enter the cytoplasmic membrane as the viral DNA passes into the cell while being converted to a double-stranded replicative form. RF replicates rapidly.
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10. What happens following the morphogenesis of the cell?
a) Progeny strands release
b) DNA replication
c) Host lysis
d) Degradation
View Answer

Answer: a
Explanation: The progeny single strands are synthesized and released from the cell as filamentous particles the following morphogenesis at the cell membrane.

11. Dominant selectable markers can be used with __________
a) Any cell type
b) Mutant cells
c) Wild-type cells
d) Recombinant cells
View Answer

Answer: a
Explanation: Endogenous markers are largely superseded by so-called dominant selectable markers, which confer a phenotype that is entirely novel to the cell and can hence be used in any cell type.

12. What are dominant selectable markers?
a) Drug-resistance genes
b) Inducing genes
c) Exogenous genes
d) Endogenous genes
View Answer

Answer: a
Explanation: The dominant selectable markers are usually drug-resistance genes of bacterial origin and transformed cell is selected on a medium that contains the drug at an appropriate concentration.

13. Methotrexate is an analog of __________
a) Aminopterin
b) Kanamycin
c) Folic acid
d) Gentamycin
View Answer

Answer: c
Explanation: Methotrexate is a folic acid analog, which is a competitive inhibitor of the enzyme dihydrofolate reductase (DHFR).

14. With respect to mammalian cell cloning, salmon sperm DNA can serve as a source of ____________
a) Non-specific carrier
b) Specific carrier
c) Genomic DNA
d) Plasmid DNA
View Answer

Answer: a
Explanation: Calcium phosphate transfection is mostly used and the specific donor DNA is often bulked with a non-specific carrier such as cleaved Salmon sperm.

15. One application in which the use of plasmid vectors is critical, in the case of mammals is ____________
a) Stable transformation
b) Transient transformation
c) Transfection
d) Transduction
View Answer

Answer: b
Explanation: One application in which the use of plasmid vectors is critical, in the case of mammals is a transient transformation. Here the goal is to exploit the short-term persistence of extrachromosomal DNA.

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Manish Bhojasia, a technology veteran with 20+ years @ Cisco & Wipro, is Founder and CTO at Sanfoundry. He lives in Bangalore, and focuses on development of Linux Kernel, SAN Technologies, Advanced C, Data Structures & Alogrithms. Stay connected with him at LinkedIn.

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