This set of Protein Engineering Multiple Choice Questions & Answers (MCQs) focuses on “Biosensors – Chemically Engineered Electronic Proteins”.
1. What do you mean by FP-redox biosensors?
a) Fluorescent protein biosensors that does not contain sulfide groups
b) Non-fluorescent protein Biosensors
c) Thiol-redox fluorescent protein biosensor
d) Thiol-redox non-fluorescent protein biosensor
View Answer
Explanation: FP-redox biosensors are fluorescent proteins that are engineered as thiol redox biosensors by introducing a couple of adjacent cysteine residues at the protein surface. The cysteine residues that act as the sensing element undergoes a local conformational change.
2. Who first discovered thiol-redox yellow fluorescent protein biosensor?
a) Jakob R. Winther
b) Richard Feynman
c) Peter Debye
d) Stephen L. Buchwald
View Answer
Explanation: Jakob R. Winther and his team discovered the first thiol-redox yellow fluorescent protein biosensor and carried out research on its applications. After which several diverse studies were conducted that resulted in the generation of new redox sensitive FP variants.
3. How does the FP-redox biosensors classified based on their photophysical properties?
a) Acoustic biosensors
b) Intensiometric and ratiometric biosensors
c) Thermometric and non-thermometric Biosensors
d) Immunosensors
View Answer
Explanation: With respect to their photophysical properties, the FP-redox biosensors can be classified into intensiometric and ratiometric biosensors. When bound to an ion, intensiometric biosensors alters fluorescent intensity while ratiometric biosensors exerts a shift in the levels of absorption and emission.
4. What do you mean by VOCs?
a) Viscous Ocular Compounds
b) Volatile Ocular Compounds
c) Viscous Organic Compounds
d) Volatile Organic Compounds
View Answer
Explanation: VOCs is the abbreviation of Volatile Organic Compounds. They have a wide range of applications as they possess high vapor pressure and low water solubility. VOCs can be detected with the help of Surface plasmon resonance imaging by using highly sensitive olfactory biosensors.
5. Which FP-redox biosensor is primarily involved in qualitative measurements?
a) Thermometric Biosensors
b) Intensiometric Biosensors
c) Acoustic Biosensors
d) Ratiometric Biosensors
View Answer
Explanation: Intensiometric Biosensors are involved mainly in qualitative measurements because the fluorescent signal emitted by these biosensors depend on the concentration of the target molecule as well as on the expression level of the biosensor.
6. The olfactory biosensors exhibits low selectivity.
a) True
b) False
View Answer
Explanation: The olfactory biosensors, typically, exhibits high selectivity. And especially when the concentration of the VOCs is lower, the selectivity range increases and would reach an extensive mark. The olfactory biosensors also show relatively high sensitivity at low concentrations.
7. Which of the following is an advantage of DNA biosensors?
a) Highly sensitive
b) Non-portable
c) Susceptible to turbidity
d) Temperature parameters can interfere the performance
View Answer
Explanation: The DNA biosensors has high sensitivity and thus their response time is faster. Several types of DNA biosensors include electrochemical, piezoelectric, laser interferometry, fiber optics and DNA chips.
8. Proteins having an increased number of amino acids like tryptophan, tyrosine and phenylalanine does not show autofluorescence.
a) True
b) False
View Answer
Explanation: In general, Protein molecules that contains amino acids like tryptophan, tyrosine and phenylalanine in increased quantity shows autofluorescence to some extent. So, the given statement is false. Molecule that exhibits autofluorescence include flavins and NADPG.
9. Which of the following statement is true regarding OBP?
a) Peptides are used instead of OBPs
b) They cannot be easily expressed
c) They consume more time
d) OBPs are diverse in nature
View Answer
Explanation: OBPs can be easily expressed and often consumes lesser time, but the process has complications as they cannot be generated through simpler means and lacks diversity. Hence peptides are used instead of OBPs.
10. What do you mean by OBP?
a) Odorant-binding proteins
b) Ocular-binding proteins
c) On-base proteins
d) Orderly-binding proteins
View Answer
Explanation: The abbreviation OBP denotes Odorant-binding proteins, a class of Olfactory proteins. They are small in size usually ranging between 10 to 30 kDa and are primarily found in vertebrates and insects. An example of OBP is RpipOBP (Rana pipiens OBP).
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