This set of Protein Engineering Multiple Choice Questions & Answers (MCQs) focuses on “Targets – Industrial Enzymology”.
1. Which of the following enzyme is not used as a target for protein engineering in detergents?
a) Proteases
b) Lipases
c) Amylases
d) Hydrolases
View Answer
Explanation: Hydrolases are not used as a target for protein engineering in detergents. Proteases, lipases, and amylases are important enzymes that are used in detergents; hence these are used as targets for protein engineering.
2. Protein engineering is a powerful tool to overcome intrinsic limitations, weakening industrial applications of available protein.
a) False
b) True
View Answer
Explanation: The above statement is true. Protein engineering is a powerful tool to overcome intrinsic limitations, weakening industrial applications of available protein. The properties to introduce in a commercial protein strongly depends on the process where this molecule has to be employed.
3. Which of the following enzymes finds no use in the textile industry?
a) Cellulases
b) Lipases
c) Catalases
d) Endonucleases
View Answer
Explanation: Endonucleases are a class of enzymes that cleave within a DNA molecule. These enzymes find no use in the textile industry. Cellulases, lipases, and catalases are important enzymes used in the textile industry.
4. Enzymes to be employed for industrial transformations are not good targets of protein engineering.
a) True
b) False
View Answer
Explanation: The above statement is false. Enzymes to be employed for industrial transformations are good targets of protein engineering and several engineered proteases, amylases, lipases, cellulases, and xylanases have been introduced in the industry.
5. Lipases are not used in which of the following industry?
a) Textile industry
b) Leader industry
c) Detergent industry
d) Iron industry
View Answer
Explanation: Lipases are not used in the iron industry. Lipases are used in the textile industry, leader industry, and the detergent industry. Lipases are a subclass of the esterases, that catalyze the hydrolysis of triacylglycerols to glycerol and fatty acids.
6. Which of the following is not a starch processing enzyme?
a) Glucoamylase
b) α – amylase
c) Glucose isomerase
d) Endonucleases
View Answer
Explanation: Endonucleases is not a starch processing enzyme. Glucoamylase, α – amylase, and glucose isomerase are starch processing enzymes. α – amylase cleaves the α-1,4-glycosidic linkages in amylose to give maltose and glucose.
7. Which of the following is the main class of industrial catalyzers?
a) Cellulases
b) Endonucleases
c) Catalases
d) Proteases
View Answer
Explanation: Proteases are the main class of industrial catalyzers, particularly because of their use in detergents. They account for over 40 % of the global enzyme market. Endonucleases, catalases, and cellulases are not the main class of industrial catalyzers.
8. Which of the following enzyme finds its application in soft drinks?
a) Hydrolase
b) Protease
c) Catalase
d) Glucose isomerase
View Answer
Explanation: Glucose isomerase enzyme finds its application in soft drinks. It is used in the conversion of corn starch to high fructose syrup for use in sweeteners, and in particular for soft drinks. Hydrolase, protease, and catalase do not find applications in the soft drink industry.
9. Which of the following enzyme fits in the blank box shown in the figure below?
a) Glucose isomerase
b) Catalase
c) Peroxidase
d) Glucoamylase
View Answer
Explanation: Glucoamylase fits in the blank box shown in the figure above. Amylases are employed to convert the starch into simpler sugars. First α – amylase yields maltodextrins which are then processed by glucoamylase giving glucose.
10. Cellulases and xylanases are not used in which of the following industry?
a) Detergent industry
b) Paper industry
c) Food processing industry
d) Starch processing industry
View Answer
Explanation: Cellulases and xylanases are not used in the starch processing industry. On the other hand, cellulases and xylanases are used in the detergent industry, paper industry, and food processing industry. Cellulases catalyzes the breakdown of cellulose.
11. Find the odd one out.
a) Cellulases
b) Pectinolytic enzymes
c) Proteases
d) Endonucleases
View Answer
Explanation: Endonuclease is not an important target of protein engineering because it is not widely used in industries. Cellulases, pectinolytic enzymes and proteases are important targets of protein engineering because they are extensively used in industries such as food processing, pharmaceutical, textiles, detergents, etc.
12. Lipases are important targets of protein engineering.
a) False
b) True
View Answer
Explanation: The above statement is true. Lipases are important targets of protein engineering. They are used in detergents and have been modified to improve their resistance to proteases in cleaning agents and to change surface properties and make them more soluble.
13. Which of the following enzyme hydrolyzes chitin?
a) Lipase
b) Endonuclease
c) Protease
d) Lysozyme
View Answer
Explanation: Lysozyme hydrolyzes chitin. It hydrolyzes the β-(1,4)-glycosidic linkages in chitin, peptidoglycan, etc. It is an industrially important enzyme. Lipase, endonuclease, and protease do not hydrolyze chitin.
14. Tolerance to temperature is one of the most desirable technical improvements in proteins.
a) False
b) True
View Answer
Explanation: The above statement is true. Tolerance to temperature is one of the most desirable technical improvements in proteins. It can be achieved through protein engineering.
15. Which of the following enzyme fits in the blank box shown in the figure below?
a) Lipase
b) Hydrolase
c) Protease
d) β – amylase
View Answer
Explanation: β – amylase enzyme fits in the blank box shown in the figure above. β – amylases catalyze the conversion of complex sugars to maltose. This enzyme hydrolyzes α-D-glycosidic linkages. Lipase, hydrolase, and protease do not fit in that box.
Sanfoundry Global Education & Learning Series – Protein Engineering.
To practice all areas of Protein Engineering, here is complete set of 1000+ Multiple Choice Questions and Answers.
If you find a mistake in question / option / answer, kindly take a screenshot and email to [email protected]
- Apply for Biotechnology Internship
- Check Protein Engineering Books
- Check Biotechnology Books
- Practice Biotechnology MCQs
